CCL8

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Recombinant Human CCL8

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
PHP297
Datasheet
E
SDS
10 µg loader

Recombinant Human CCL8

C-C motif chemokine-8 (CCL8) is also known as HC14, monocyte chemoattractant protein 2, monocytes chemotactic protein 2 and small-inducible cytokine A8. CCL8 is a member of the C-C chemokine subfamily and is an agonist for C-C chemokine receptor type 2 and CCR5 (Ge et al. 2017). Through these receptors, CCL8 is involved in the immune response by attracting monocytes, T-lymphocytes, natural killer cells, basophils, mast cells and eosinophils (Farmaki et al. 2020). Proteolytic cleavage of CCL8 to CCL8 (6-76) converts the chemokine into a potent inhibitor of C-C chemokine-induced chemotaxis (Proost et al. 1998).

This recominant Human CCL8 can be used to create a standard curve in a sandwich ELISA with purified Mouse anti Human CCL8 antibody, clone E07-8G5 (MCA6266GA) as a capture antibody and biotinylated Mouse anti Human CCL8 antibody, clone E09-8A1 (MCA6267B) as the detection antibody.

Product Details

Target Species
Human
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Centrifuge vial prior to reconstitution. Reconstitute to 100 µg/ml by adding 100 μl ddH2O.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. Do not vortex.
Preparation
Recombinant protein expressed in E. coli and purified by ion exchange chromatography
Buffer Solution
50 mM Tris-HCl, 0.15 M Sodium Chloride
Preservative Stabilisers
1.0% Trehalose
Purity
≥90% determined by coomassie blue staining of a reducing SDS-PAGE gel
Approx. Protein Concentrations
100 μg/ml after reconstitution
Protein Molecular Weight
8.9 kDa

Storage Information

Storage
Prior to reconstitution store at -70oC. Following reconstitution store at -80oC.

This product should be stored undiluted.

Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
Guaranteed for 3 months from the date of reconstitution or until the date of expiry, whichever comes first. Please see label for expiry date.

More Information

UniProt
P80075
Entrez Gene
CCL8
GO Terms
GO:0009615 response to virus
GO:0004871 signal transducer activity
GO:0005615 extracellular space
GO:0006816 calcium ion transport
GO:0006887 exocytosis
GO:0006935 chemotaxis
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007267 cell-cell signaling
GO:0008009 chemokine activity
GO:0008201 heparin binding
Regulatory
For research purposes only

Applications of CCL8

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human CCL8 MCA6266GA E 0.1 mg loader
Mouse anti Human CCL8:Biotin MCA6267B E 0.1 mg loader

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