Apolipoprotein A1

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Native Human Apolipoprotein A1

Product Type
Purified Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
0650-0311 E 0.5 mg
Native Human apolipoprotein A1 can be used for coating microplates and as a ligand for immunosorbent preparation.

Apolipoproteins are lipid-binding proteins which enable the transport of dietary lipids for storage, metabolism and secretion. Apolipoprotein A-1 (also known as Apo-A1) plays an important part in the removal of cholesterol from cells.

Product Details

Target Species
Human
Product Form
Purified apolipoprotein A-1 from human plasma - lyophilized
Reconstitution
Reconstitute with 1.0 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Preparation
Purified human Apo A1 prepared by ultracentrifugation, delipidation and gel filtration
Buffer Solution
0.05 M Sodium chloride, 0.01 M Sodium carbonate
Preservative Stabilisers
None present
Purity
>90% by SDS PAGE
Protein Molecular Weight
28 kDa

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein.
Shelf Life
Please see label for expiry date.

More Information

UniProt
P02647 Related reagents
Entrez Gene
APOA1 Related reagents
GO Terms
GO:0005886 plasma membrane
GO:0001540 beta-amyloid binding
GO:0002576 platelet degranulation
GO:0002740 negative regulation of cytokine secretion involved in immune response
GO:0005543 phospholipid binding
GO:0005788 endoplasmic reticulum lumen
GO:0030141 stored secretory granule
GO:0030139 endocytic vesicle
GO:0006656 phosphatidylcholine biosynthetic process
GO:0007186 G-protein coupled receptor protein signaling pathway
GO:0042802 identical protein binding
GO:0010873 positive regulation of cholesterol esterification
GO:0010903 negative regulation of very-low-density lipoprotein particle remodeling
GO:0015485 cholesterol binding
GO:0050821 protein stabilization
GO:0017127 cholesterol transporter activity
GO:0019899 enzyme binding
GO:0030168 platelet activation
GO:0032488 Cdc42 protein signal transduction
GO:0033344 cholesterol efflux
GO:0033700 phospholipid efflux
GO:0034191 apolipoprotein A-I receptor binding
GO:0034361 very-low-density lipoprotein particle
GO:0034366 spherical high-density lipoprotein particle
GO:0034375 high-density lipoprotein particle remodeling
GO:0034380 high-density lipoprotein particle assembly
GO:0034384 high-density lipoprotein particle clearance
GO:0042632 cholesterol homeostasis
GO:0043691 reverse cholesterol transport
GO:0050713 negative regulation of interleukin-1 beta secretion
GO:0051345 positive regulation of hydrolase activity
GO:0060228 phosphatidylcholine-sterol O-acyltransferase activator activity
GO:0070508 cholesterol import
GO:0070653 high-density lipoprotein particle receptor binding
Regulatory
For research purposes only

Applications of Apolipoprotein A1

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Product Specific References

References for Apolipoprotein A1

  1. Tall, A.R. & Small, D.M. (1980) Body cholesterol removal: role of plasma high-density lipoproteins.
    Adv Lipid Res. 17: 1-51.
  2. Fielding, C.J. (1972) A protein cofactor of lecithin:cholesterol acyltransferase.
    Biochem. Biophys. Res. Commun. 46: 1493-1498.