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Alpha 1 Microglobulin

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Native Human Alpha 1 Microglobulin

Product Type
Purified Protein
Specificity
Alpha 1 Microglobulin

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Product Code Applications Pack Size List Price Your Price Qty
6220-1004
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SDS Safety Datasheet SDS
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Native Human alpha 1 microglobulin is native human Alpha-1-Microglobulin, a secreted peptide thought to play a role in the regulation of inflammatory processes.

This product runs at approximately 27 kDa under reducing conditions and as a dimer at approximately 54 kDa under non-reducing conditions.

Target Species
Human
Product Form
Purified protein from the urine of patients with chronic renal tubular proteinuria - lyophilized
Reconstitution
Reconstitute with 1.0ml sterile phosphate buffered saline (PBS).
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Purified Alpha 1 Microglobulin prepared by a combination of ion exchange, affinity and size exclusion chromatography.
Preservative Stabilisers
None present
Purity
>96% by SDS PAGE
Approx. Protein Concentrations
Total protein concentration 1.0mg/ml after reconstitution.
Regulatory
For research purposes only
Guarantee
Guaranteed until date of expiry. Please see product label.

Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

References for Alpha 1 Microglobulin

  1. Weyer, K. et al. (2013) Renal uptake of 99mTc-dimercaptosuccinic acid is dependent on normal proximal tubule receptor-mediated endocytosis.
    J Nucl Med. 54 (1): 159-65.

Synonyms
AMBP
UniProt
P02760
Entrez Gene
AMBP
GO Terms
GO:0005886 plasma membrane
GO:0007155 cell adhesion
GO:0005576 extracellular region
GO:0004867 serine-type endopeptidase inhibitor activity
GO:0005215 transporter activity
GO:0007565 female pregnancy
GO:0018298 protein-chromophore linkage
GO:0019855 calcium channel inhibitor activity
GO:0019862 IgA binding
GO:0020037 heme binding
GO:0042167 heme catabolic process
GO:0042803 protein homodimerization activity
GO:0044419 interspecies interaction between organisms
GO:0046329 negative regulation of JNK cascade
GO:0046904 calcium oxalate binding
GO:0050777 negative regulation of immune response

6220-1004

148304 157143 159739 162642 163694 165878

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