IFN Gamma
Recombinant Dog interferon gamma, (IFNγ), known previously as macrophage activating factor, belongs to the type II interferon family and is produced by T lymphocytes and NK cells.
Native dog interferon gamma is a 166 amino acid cytokine with potent activating, anti-viral and anti-proliferative properties.
IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection and has numerous immunological regulatory functions. Properties include macrophage and NK cell activation, upregulation of MHC class I and II molecules on various cells and induction of cytotoxicity.
Native dog interferon gamma is a 166 amino acid cytokine with potent activating, anti-viral and anti-proliferative properties.
IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection and has numerous immunological regulatory functions. Properties include macrophage and NK cell activation, upregulation of MHC class I and II molecules on various cells and induction of cytotoxicity.
Product Details
- Target Species
- Dog
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 10% Trehalose
- Purity
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- 16.7 kDa
Storage Information
- Storage
- Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. - Shelf Life
- 12 months from date of despatch
More Information
- UniProt
- P51742 Related reagents
- Entrez Gene
- TNF Related reagents
- GO Terms
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
- Regulatory
- For research purposes only
Applications of IFN Gamma
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Western Blotting |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
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