IFN Gamma
Recombinant Dog Interferon gamma (PDP010) detected using Rabbit anti Dog interferon gamma (AHP2977 ) as the capture reagent and Rabbit anti Dog Interferon gamma:Biotin (AHP2977B ) as the detection reagent followed by Streptavidin:HRP
Recombinant Dog interferon gamma (PDP010) used to stimulate DH82 cells in vitro prior to measurement of reactive oxygen species (ROS) production.
Image caption:
Reactive species of oxygen (ROS) production by DH82 cells treated with purified capsular polysaccharides from C. neoformans. DH82 canine macrophages were cultured (5 × 104/mL) and incubated with H2DCFDA, followed by washing. The cells were treated with GXMGal, GXM, or capsule (100 μg/mL), and some cultures were stimulated with LPS (400 ng/mL) and IFN-γ (1.5 ng/mL) for 24 h. Control represents unstimulated samples that were not treated with polysaccharides. The data represent the mean ± SEM of three independent experiments, conducted in triplicates. Statistical analysis was performed by variance (ANOVA), followed by Dunnett’s post-test. We considered significant differences indicated between ** p <0.01 and *** p <0.001.
From: LaRocque-de-Freitas IF, da Silva-Junior EB, Gemieski LP, da Silva Dias Lima B, Diniz-Lima I, de Carvalho Vivarini A, Lopes UG, Freire-de-Lima L, Morrot A, Previato JO, Mendonça-Previato L, Pinto-da-Silva LH, Freire-de-Lima CG, Decote-Ricardo D.
Inhibition of Microbicidal Activity of Canine Macrophages DH82 Cell Line by Capsular Polysaccharides from Cryptococcus neoformans.
J Fungi (Basel). 2024 May 8;10(5):339.
doi: 10.3390/jof10050339.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Recombinant Dog interferon gamma (PDP010) used to stimulate DH82 cells in vitro prior to measurement of MHC Class II expression by flow cytometry.
Image caption:
Measurement of MHC class II expression in canine macrophages DH82 treated with polysaccharides from C. neoformans. DH82 macrophages (5 × 105/mL) were treated with GXMGal, GXM, or capsule (100 μg/mL). Some cultures were stimulated with LPS (400 ng/mL) and IFN-γ (1.5 ng/mL). Control represents unstimulated samples that were not treated with polysaccharides. After 24 h, cells were prepared for analysis by flow cytometry. M1 marker shows percentage of cells identified by MHC class II (A,B). Data represent mean ± SEM of three independent experiments, conducted in triplicates. Statistical analysis was performed by variance (ANOVA), followed by Dunnett’s post-test. We considered significant differences indicated by *** p <0.001.
From: LaRocque-de-Freitas IF, da Silva-Junior EB, Gemieski LP, da Silva Dias Lima B, Diniz-Lima I, de Carvalho Vivarini A, Lopes UG, Freire-de-Lima L, Morrot A, Previato JO, Mendonça-Previato L, Pinto-da-Silva LH, Freire-de-Lima CG, Decote-Ricardo D.
Inhibition of Microbicidal Activity of Canine Macrophages DH82 Cell Line by Capsular Polysaccharides from Cryptococcus neoformans.
J Fungi (Basel). 2024 May 8;10(5):339.
doi: 10.3390/jof10050339.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Recombinant Dog interferon gamma (PDP010) used to stimulate DH82 cells in vitro prior to measurement of cytokine mRNA by qRT-PCR.
Image caption:
Cytokine mRNA expression of the canine macrophage DH82. Cytokine profile in canine macrophage. DH82 cells (1 × 106/mL) were plated and treated with GXMGal, GXM, and capsule (100 μg/mL). Some cultures were stimulated with LPS (400 ng/mL) and IFN-γ (1.5 ng/mL). Control represents unstimulated samples that were not treated with polysaccharides. After 24 h of incubation, the cells were processed, and mRNA for the cytokines was quantified by qRT-PCR. (A) mRNA expression of IL-12, (B) mRNA expression of IL-6, (C) mRNA expression of TGF-β. Data from the experiments were normalized using GADPH primers as an endogenous control. The data represent the mean ± SEM of three independent experiments, conducted in triplicates. Statistical analysis was performed by variance (ANOVA), followed by Dunnett’s post-test. We considered significant differences indicated between ** p<0.01 and *** p <0.001.
From: LaRocque-de-Freitas IF, da Silva-Junior EB, Gemieski LP, da Silva Dias Lima B, Diniz-Lima I, de Carvalho Vivarini A, Lopes UG, Freire-de-Lima L, Morrot A, Previato JO, Mendonça-Previato L, Pinto-da-Silva LH, Freire-de-Lima CG, Decote-Ricardo D.
Inhibition of Microbicidal Activity of Canine Macrophages DH82 Cell Line by Capsular Polysaccharides from Cryptococcus neoformans.
J Fungi (Basel). 2024 May 8;10(5):339.
doi: 10.3390/jof10050339.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Recombinant Dog interferon gamma (PDP010) used to stimulate DH82 cells in vitro prior to evaluation of MAPK phosphorylation and PPAR- γ activation by western blotting.
Image caption:
MAPK phosphorylation and PPAR- γ activation. Canine macrophages DH82 (5 × 105) were plated and treated with GXMGal, GXM, and capsule (100 μg/mL). Some cultures were stimulated with LPS and IFN-γ. Control represents unstimulated samples that were not treated with polysaccharides. After 24 h of incubation, whole-cell lysates were loaded onto SDS-PAGE gels. The blot was run and probed with the following antibodies: (A) anti-phospho ERK antibody, (B) anti-PPAR-γ antibody and (C) anti-PPAR-γ. The same blots were then stripped and reprobed with antibodies to non-phosphorylated proteins to determine absolute protein levels. Bar graphics show the ratio of phosphorylated and total proteins. The band densitometry of Western blotting was analyzed using the Scion Image software 4.0.3.2. The data represent the mean ± SEM of three independent experiments, conducted in triplicates. Statistical analysis was performed by variance (ANOVA), followed by Dunnett’s post-test. We considered significant differences indicated between * p <0.05, ** p <0.01 and *** p <0.001.
From: LaRocque-de-Freitas IF, da Silva-Junior EB, Gemieski LP, da Silva Dias Lima B, Diniz-Lima I, de Carvalho Vivarini A, Lopes UG, Freire-de-Lima L, Morrot A, Previato JO, Mendonça-Previato L, Pinto-da-Silva LH, Freire-de-Lima CG, Decote-Ricardo D.
Inhibition of Microbicidal Activity of Canine Macrophages DH82 Cell Line by Capsular Polysaccharides from Cryptococcus neoformans.
J Fungi (Basel). 2024 May 8;10(5):339.
doi: 10.3390/jof10050339.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Recombinant Dog Interferon Gamma
- Product Type
- Recombinant Protein
- Specificity
- IFN Gamma
Antibody Quality and Performance Guarantee
| Recombinant Dog interferon gamma, (IFNγ), known previously as macrophage activating factor, belongs to the type II interferon family and is produced by T lymphocytes and NK cells. Native dog interferon gamma is a 166 amino acid cytokine with potent activating, anti-viral and anti-proliferative properties. IFNγ is predominantly secreted by activated T lymphocytes in response to specific mitogens as a result of infection and has numerous immunological regulatory functions. Properties include macrophage and NK cell activation, upregulation of MHC class I and II molecules on various cells and induction of cytotoxicity. |
- Target Species
- Dog
- Product Form
- Purified recombinant protein - lyophilized
- Reconstitution
- Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. - Preparation
- Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
- Source
- Pichia pastoris
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 10% Trehalose
- Purity
- >95% by SDS PAGE analysis
- Protein Molecular Weight
- 16.7 kDa
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
References for IFN Gamma
-
LaRocque-de-Freitas, I.F. et al. (2024) Inhibition of Microbicidal Activity of Canine Macrophages DH82 Cell Line by Capsular Polysaccharides from Cryptococcus neoformans.
J Fungi (Basel). 10 (5) May 08 [Epub ahead of print].
- Synonyms
- IFNG
- UniProt
- P51742
- Entrez Gene
- TNF
- GO Terms
- GO:0005125 cytokine activity
- GO:0005615 extracellular space
View more products with IFN GAMMA specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Dog ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up