IL-1 Beta

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Recombinant Chicken Interleukin-1 beta

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PAP002 FN IA 50 µg
Recombinant Chicken interleukin-1 (IL-1) is a pro-inflammatory cytokine secreted by many different cell types acting on a wide range of target cells, resulting in a diverse range of effects. The biological properties of IL-1 include the induction of B cell proliferation, stimulation of T cell proliferation in conjunction with IL-2 and the production of collagenase and prostaglandin by fibroblasts. IL-1 is also responsible for triggering the acute-phase response in the liver, and is involved in both cytokine network and vascular endothelium activation. Recombinant Chicken interleukin-1 (PAP002) induces corticosterone secretion in vivo (Weining et al. 1992).

Recombinant Chicken interleukin-1 (PAP002) was the immunogen used to produce Bio-Rad chicken IL-1beta antibody (AHP941Z).

Product Details

Target Species
Chicken
Product Form
Purified His-tagged recombinant protein expressed in E.coli - liquid
Preparation
Recombinant protein prepared from E.coli
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Purity
>85% by Ni chelate Chromatography
Approx. Protein Concentrations
250 ug/ml
Protein Molecular Weight
approximately 23kD

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of despatch.

More Information

UniProt
O73909 Related reagents
Acknowledgements
His-tag is a registered trademark of EMD Biosciences.
Regulatory
For research purposes only

Applications of IL-1 Beta

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
In vitro Assay
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Product Specific References

References for IL-1 Beta

  1. Weining, K.C. et al. (1998) A chicken homolog of mammalian interleukin-1 beta: cDNA cloning and purification of active recombinant protein.
    Eur J Biochem. 258 (3): 994-1000.
  2. Gyorfy, Z. et al. (2003) Truncated chicken interleukin-1beta with increased biologic activity.
    J Interferon Cytokine Res. 23 (5): 223-8.
  3. Abdel-mageed, A.M. et al. (2017) Modulatory roles of proinflammatory cytokines on the expression of cathelicidins in the lower regions of the oviduct of laying hens.
    Cytokine. 99: 66-72.