IFN Alpha

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Recombinant Chicken Interferon Alpha

Product Type
Recombinant Protein
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
PAP004 FN 50 µg
Recombinant Chicken interferon alpha (IFN alpha) shares structural and biological properties with mammalian IFN1. Recombinant chicken IFN alpha (rChIFNa) has been shown to act as a highly potent anti-viral agent in vitro (Schultz, U. et al. 1995). rChIFNa also resulted in a noticeable delay in the progression of tumours in CC progressor chickens infected with Rous Sarcoma Virus, when admimistered intravenously (Plachy, J. et al. 1999).

Recombinant chicken interferon alpha is the immunogen used to produce the polyclonal Rabbit anti Chicken interferon alpha antibody.

Product Details

Target Species
Chicken
Product Form
Purified recombinant protein expressed in E.coli - liquid.
Preparation
Recombinant protein prepared from E.coli
Buffer Solution
0.05M Sodium Acetate
1M Sodium Chloride
2M Urea
10mM Beta-Mercaptoethanol
Preservative Stabilisers
None present
Activity
1x107units/mg
Purity
>85% by Ni chelate chromatography
Approx. Protein Concentrations
Total protein concentration 1.0mg/ml
Protein Molecular Weight
19kD

Storage Information

Storage
Store at -20oC only.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein.
This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
3 months from date of despatch.

More Information

UniProt
P42165 Related reagents
Entrez Gene
IFNA3 Related reagents
GO Terms
GO:0006952 defense response
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005126 cytokine receptor binding
GO:0005615 extracellular space
Regulatory
For research purposes only

Applications of IFN Alpha

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Application Based External Images

Functional Assays

Product Specific References

References for IFN Alpha

  1. Schultz, U. et al. (1995) Recombinant chicken interferon: a potent antiviral agent that lacks intrinsic macrophage activating factor activity.
    Eur J Immunol. 25 (3): 847-51.
  2. Plachy, J. et al. (1999) Protective effects of Type I and Type II interferons toward Rous Sarcoma Virus-induced tumors in chickens.
    Virology. 256: 85-91.
  3. Schwarz, H. et al. (2004) Synthesis of IFN-beta by virus-infected chicken embryo cells demonstrated with specific antisera and a new bioassay.
    J Interferon Cytokine Res. 24 (3): 179-84.
  4. Schultz, U. et al. (1995) Recombinant chicken interferon from Escherichia coli and transfected COS cells is biologically active.
    Eur J Biochem. 229 (1): 73-6.
  5. Jiang, H. et al. (2011) Chicken interferon alpha pretreatment reduces virus replication of pandemic H1N1 and H5N9 avian influenza viruses in lung cell cultures from different avian species.
    Virol J. 8: 447.
  6. Smith, S.E. et al. (2013) Chicken interferon-inducible transmembrane protein 3 restricts influenza viruses and lyssa viruses in vitro.
    J Virol. 87 (23): 12957-66.
  7. Alamares, J.G. et al. (2010) The interferon antagonistic activities of the V proteins from two strains of Newcastle disease virus correlate with their known virulence properties.
    Virus Res. 147 (1): 153-7.
  8. Lostalé-Seijo Irene et al. (2016) Interferon induction by avian reovirus
    Virology. 487: 104-111.
  9. Lostalé-Seijo I et al. (2016) Response of Three Different Viruses to Interferon Priming and Dithiothreitol Treatment of Avian Cells.
    J Virol. Jul 20. pii: JVI.01175-16. [Epub ahead of print]
  10. Deng, L. et al. (2016) Characterization and functional studies of fowl adenovirus 9 dUTPase.
    Virology. 497: 251-61.