IFN Alpha

Recombinant Chicken Interferon Alpha

Product Type
Recombinant Protein
Specificity
IFN Alpha

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PAP004
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Recombinant Chicken interferon alpha (IFN alpha) shares structural and biological properties with mammalian IFN1. Recombinant chicken IFN alpha (rChIFNa) has been shown to act as a highly potent anti-viral agent in vitro (Schultz, U. et al. 1995). rChIFNa also resulted in a noticeable delay in the progression of tumors in CC progressor chickens infected with Rous Sarcoma Virus, when administered intravenously (Plachy, J. et al. 1999).

Recombinant chicken interferon alpha is the immunogen used to produce the polyclonal Rabbit anti Chicken interferon alpha antibody.

Target Species
Chicken
Product Form
Purified recombinant protein expressed in E.coli - liquid.
Preparation
Recombinant protein prepared from E.coli
Buffer Solution
0.05M Sodium Acetate
1M Sodium Chloride
0.01M Tris-HCl
2M Urea
10mM Beta-Mercaptoethanol
Preservative Stabilisers
None present
Activity
1x107units/mg
Purity
>85% by Ni chelate chromatography
Approx. Protein Concentrations
Total protein concentration 1.0 mg/ml
Protein Molecular Weight
19 kD
Regulatory
For research purposes only
Guarantee
3 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein.
This product should be stored undiluted.
Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Where this protein has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for IFN Alpha

  1. Schultz, U. et al. (1995) Recombinant chicken interferon: a potent antiviral agent that lacks intrinsic macrophage activating factor activity.
    Eur J Immunol. 25 (3): 847-51.
  2. Plachy, J. et al. (1999) Protective effects of Type I and Type II interferons toward Rous Sarcoma Virus-induced tumors in chickens.
    Virology. 256: 85-91.
  3. Schwarz, H. et al. (2004) Synthesis of IFN-beta by virus-infected chicken embryo cells demonstrated with specific antisera and a new bioassay.
    J Interferon Cytokine Res. 24 (3): 179-84.
  4. Schultz, U. et al. (1995) Recombinant chicken interferon from Escherichia coli and transfected COS cells is biologically active.
    Eur J Biochem. 229 (1): 73-6.
  5. Jiang, H. et al. (2011) Chicken interferon alpha pretreatment reduces virus replication of pandemic H1N1 and H5N9 avian influenza viruses in lung cell cultures from different avian species.
    Virol J. 8: 447.
  6. Smith, S.E. et al. (2013) Chicken interferon-inducible transmembrane protein 3 restricts influenza viruses and lyssa viruses in vitro.
    J Virol. 87 (23): 12957-66.
  7. Alamares, J.G. et al. (2010) The interferon antagonistic activities of the V proteins from two strains of Newcastle disease virus correlate with their known virulence properties.
    Virus Res. 147 (1): 153-7.
  8. Lostalé-seijo, I. et al. (2016) Interferon induction by avian reovirus.
    Virology. 487: 104-11.
  9. View The Latest Product References
  10. Lostalé-seijo, I. et al. (2016) Response of Three Different Viruses to Interferon Priming and Dithiothreitol Treatment of Avian Cells.
    J Virol. 90 (18): 8328-40.
  11. Deng, L. et al. (2016) Characterization and functional studies of fowl adenovirus 9 dUTPase.
    Virology. 497: 251-61.

Functional Assays

Synonyms
Interferon Alpha
UniProt
P42165
Entrez Gene
IFNA3
GO Terms
GO:0006952 defense response
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005126 cytokine receptor binding
GO:0005615 extracellular space
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