IFN Gamma

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Recombinant Cat IFN Gamma

Product Type
Recombinant Protein
Product Code Applications Pack Size List Price Quantity
PFP004
Datasheet
E WB
MSDS
5 µg loader

Recombinant Cat Interferon gamma is a recombinant protein corresponding to aa 24-167 aa of native cat interferon gamma.

IFN gamma known previously as macrophage activating factor, belongs to the type II interferon family and is produced by activated T lymphocytes and NK cells.

IFN gamma is a multipotent cytokine, has a broad range of regulatory functions during virus induced host immune response. IFN gamma promotes a Th1-type immune response in CD4+ and CD8+T cells and influences differentiation of T cells (Ochi et al. 2008).

Product Details

Target Species
Cat
Product Form
Purified recombinant protein - lyophilized
Reconstitution
Reconstitute with 20 μl sterile PBS containing 0.1% carrier protein
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
Preparation
Recombinant protein expressed in Pichia pastoris and purified by ion-exchange chromatography
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
10% Trehalose
Purity
>95% by SDS PAGE analysis
Protein Molecular Weight
17.0 kDa

Storage Information

Storage
Prior to reconstitution store at -20oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted.
Guarantee
12 months from date of despatch

More Information

UniProt
P46402
Entrez Gene
IFNG
GO Terms
GO:0009615 response to virus
GO:0005125 cytokine activity
GO:0005133 interferon-gamma receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
Regulatory
For research purposes only

Applications of IFN Gamma

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This reagent may be used as a standard in a sandwich ELISA assay in conjunction with AHP2834 as a coating antibody and AHP2834B as a detection antibody.