IL-8 antibody
Rabbit anti Human Interleukin-8:Biotin
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- IL-8
Antibody Quality and Performance Guarantee
| Rabbit anti Human Interleukin-8 antibody recognizes human IL-8 (Interleukin-8), a 72 amino acid pro-inflammatory CXC chemokine designated CXCL8, secreted by endothelial cells and monocytes, which acts as a major chemoattractant for neutrophils and also T cells and basophils. IL-8, first isolated from osteosarcoma cells, contains the ELR-motif (N-Terminal Glu-Leu-Arg amino acid sequence) and signals through the CXCR1 and CXCR2 receptors. Previous nomenclature for IL-8 includes neutrophil activating protein 1(NAP-1), granulocyte chemotactic protein 1 (GCP-1), monocyte-derived neutrophil-activating peptide (MONAP) and protein 3-10C. |
- Target Species
- Human
- Product Form
- Purified IgG conjugated to Biotin - lyophilized
- Reconstitution
- Reconstitute with 0.5 ml sterile PBS containing 0.1% Bovine Serum Albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution.
For long term storage the addition of 0.09% sodium azide is recommended. - Antiserum Preparation
- Antisera to human IL-8 were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Carrier Free
- Yes
- Immunogen
- Recombinant human IL-8 (PHP055)
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml after reconstitution
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
After reconstitution store at -20°C.
This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
0.25 | 1.0ug/ml |
| Western Blotting | |
0.1 | 0.2ug/ml |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own systems using appropriate negative/positive controls.
- ELISA
- This biotinylated human IL-8 antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with a purified human IL-8 antibody (AHP781) as the capture reagent and recombinant human IL-8 (PHP055) as the standard.
- Western Blotting
- This biotinylated human IL-8 antibody may be used in Western Blotting applications under either reducing or non-reducing conditions with recombinant human IL-8 (PHP055) as the positive control.
References for IL-8 antibody
-
Vazquez, G.R. et al. (2018) Adipocytes Promote Early Steps of Breast Cancer Cell Dissemination via Interleukin-8.
Front Immunol. 9: 1767. -
Fu, X. et al. (2023) High FOXA1 levels induce ER transcriptional reprogramming, a pro-metastatic secretome, and metastasis in endocrine-resistant breast cancer.
Cell Rep. 42 (8): 112821.
Further Reading
-
Mukaida, N. et al. (1989) Genomic structure of the human monocyte-derived neutrophil chemotactic factor IL-8.
J Immunol. 143 (4): 1366-71.
- Synonyms
- CXCL8
- RRID
- AB_2126224
- UniProt
- P10145
- Entrez Gene
- IL8
AHP781B
100914
164820
220714
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
View more products with IL-8 specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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