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CD120b antibody

Rabbit anti Human CD120b:Biotin

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CD120b

Product Code Applications Pack Size List Price Your Price Qty
AHP1833B
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SDS Safety Datasheet SDS
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Rabbit anti Human CD120b recognizes the soluble form of human CD120b, otherwise known as sTNF receptor type II (sTNFR1I), a member of the tumor necrosis factor receptor (TNFR) superfamily, produced by proteolytic cleavage of the membrane form by the metalloprotease TACE.

CD120b plays a key role in regulating the inflammatory response, acting as a receptor for both tumour necrosis factor alpha (TNF-alpha) and TNF-beta, and is also involved in programmed cell death and anti-viral activity. sTNFR1I acts as an inhibitor of TNF-alpha induced responses, by acting as a decoy receptor.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - lyophilized
Reconstitution
Reconstitute with 0.5ml sterile PBS containing 0.1% bovine serum albumin.
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Antiserum Preparation
Antiserum to human CD120b were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
None present
Carrier Free
Yes
Immunogen
Recombinant human CD120b.
Approx. Protein Concentrations
IgG concentration 0.1mg/ml after reconstitution
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Prior to reconstitution store at -20°C.
After reconstitution store at -20°C.

This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 0.25 1.0ug/ml
Western Blotting 0.1 0.2ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
ELISA
This biotinylated human CD120b antibody may be used in a direct ELISA or as the detection reagent in a sandwich ELISA with a purified human CD120b antibody (AHP1833) as the capture reagent.

Synonyms
TNF-R2
RRID
AB_2206652
UniProt
P20333
Entrez Gene
TNFRSF1B
GO Terms
GO:0005886 plasma membrane
GO:0006915 apoptosis
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0005031 tumor necrosis factor receptor activity
GO:0045121 membrane raft

AHP1833B

148279 148505 148570 148593 159541 164817

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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