VSV-G Tag antibody
Rabbit anti VSV-G Tag:Biotin
- Product Type
- Polyclonal Antibody
- Isotype
- Polyclonal IgG
- Specificity
- VSV-G Tag
Antibody Quality and Performance Guarantee
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| Rabbit anti VSV-G Tag antibody recognizes recombinant proteins containing the Vesicular Stomatitis Virus Glycoprotein (VSV-G) epitope tag. Epitope tags are short peptide sequences that can be attached to proteins from all species, and do not affect the protein’s biochemical properties. VSV is a member of the Rhabdoviridae family, and consists of a single RNA molecule that encodes five proteins, one of which is a surface glycoprotein (G). G is a type I membrane glycoprotein and is responsible for viral binding to cell surface receptors and initiating the infectious process. Rabbit anti VSV-G Tag antibody is suitable for immunofluorescence. However, filamentous staining may be observed at dilutions lower than 1:200. |
- Target Species
- Viral
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Antiserum Preparation
- Antisera to VSV-G were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- <0.1% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- Synthetic peptide corresponding to the carboxy terminal of vesicular stomatitis virus glycoprotein (VSV-G)
- Approx. Protein Concentrations
- IgG concentration 0.5mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
1/3000 | 1/12000 |
| Immunofluorescence | |
1/500 | 1/2000 |
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
Further Reading
-
Dreja, H. & Piechaczyk, M. (2006) The effects of N-terminal insertion into VSV-G of an scFv peptide.
Virol J. 3: 69. -
Barber, G.N. (2005) VSV-tumor selective replication and protein translation.
Oncogene. 24 (52): 7710-9.
- Synonyms
- Vesicular Stomatitis Virus Glycoprotein
- RRID
- AB_872051
AHP1228B
147950
155736
167602
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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