TGF Beta antibody

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Rabbit anti Human TGF Beta

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1734 E P* WB 50 µg
Rabbit anti Human TGF beta antibody recognizes human Transforming Growth Factor Beta 1 (TGF-beta1), a 390 amino acid secreted protein belonging to the TGF-beta family.

TGF-beta1 is a multifunctional protein that controls proliferation, differentiation and other functions in a wide range of cell types and both positively and negatively regulates many other growth factors. TGF-beta1 also plays an important role in bone remodelling through stimulation of oesteoblastic bone formation causing chemotaxis, proliferation and differentiation in committed osteoblasts.

Defects in TGFB1 are the cause of Camurati-Engelmann disease (CED), also known as progressive diaphyseal dysplasia 1 (Kinoshita et al. 2000).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mammals Expected from Sequence
Rabbit
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Human TGF-beta1 were raised by repeated immunisation of Rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
Immunogen
A synthetic peptide corresponding to amino acids 286-293 of the human TGF-beta precursor protein.
Approx. Protein Concentrations
IgG concentration 0.4 mg/ml.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01137 Related reagents
Entrez Gene
TGFB1 Related reagents
GO Terms
GO:0016049 cell growth
GO:0001933 negative regulation of protein phosphorylation
GO:0002062 chondrocyte differentiation
GO:0002244 hemopoietic progenitor cell differentiation
GO:0002248 connective tissue replacement involved in inflammatory response wound healing
GO:0002576 platelet degranulation
GO:0005114 type II transforming growth factor beta receptor binding
GO:0005578 proteinaceous extracellular matrix
GO:0005615 extracellular space
GO:0005634 nucleus
GO:0005796 Golgi lumen
GO:0006611 protein export from nucleus
GO:0006754 ATP biosynthetic process
GO:0006917 induction of apoptosis
GO:0007050 cell cycle arrest
GO:0007173 epidermal growth factor receptor signaling pathway
GO:0007179 transforming growth factor beta receptor signaling pathway
GO:0007183 SMAD protein complex assembly
GO:0007184 SMAD protein import into nucleus
GO:0007435 salivary gland morphogenesis
GO:0048535 lymph node development
GO:0008083 growth factor activity
GO:0008156 negative regulation of DNA replication
GO:0010718 positive regulation of epithelial to mesenchymal transition
GO:0010742 macrophage derived foam cell differentiation
GO:0010763 positive regulation of fibroblast migration
GO:0010800 positive regulation of peptidyl-threonine phosphorylation
GO:0010862 positive regulation of pathway-restricted SMAD protein phosphorylation
GO:0016202 regulation of striated muscle tissue development
GO:0016563 transcription activator activity
GO:0017015 regulation of transforming growth factor beta receptor signaling pathway
GO:0019049 evasion of host defenses by virus
GO:0019058 viral infectious cycle
GO:0022408 negative regulation of cell-cell adhesion
GO:0030168 platelet activation
GO:0030308 negative regulation of cell growth
GO:0030501 positive regulation of bone mineralization
GO:0031093 platelet alpha granule lumen
GO:0031575 mitotic cell cycle G1/S transition checkpoint
GO:0032355 response to estradiol stimulus
GO:0032570 response to progesterone stimulus
GO:0032740 positive regulation of interleukin-17 production
GO:0032801 receptor catabolic process
GO:0032967 positive regulation of collagen biosynthetic process
GO:0033138 positive regulation of peptidyl-serine phosphorylation
GO:0035307 positive regulation of protein dephosphorylation
GO:0043406 positive regulation of MAP kinase activity
GO:0043536 positive regulation of blood vessel endothelial cell migration
GO:0043537 negative regulation of blood vessel endothelial cell migration
GO:0043552 positive regulation of phosphatidylinositol 3-kinase activity
GO:0043932 ossification involved in bone remodeling
GO:0045216 cell-cell junction organization
GO:0045599 negative regulation of fat cell differentiation
GO:0045930 negative regulation of mitotic cell cycle
GO:0046732 active induction of host immune response by virus
GO:0048298 positive regulation of isotype switching to IgA isotypes
GO:0050680 negative regulation of epithelial cell proliferation
GO:0050714 positive regulation of protein secretion
GO:0050921 positive regulation of chemotaxis
GO:0051781 positive regulation of cell division
GO:0051897 positive regulation of protein kinase B signaling cascade
GO:0060389 pathway-restricted SMAD protein phosphorylation
GO:0060391 positive regulation of SMAD protein import into nucleus
GO:0070723 response to cholesterol
Regulatory
For research purposes only

Applications of TGF Beta antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/10000
Immunohistology - Paraffin 1 5ug/ml
Western Blotting 1/200 1/1000
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human spleen

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
Histar Detection System STAR3000A 50 Tests C P

Application Based External Images

Immunohistology - Paraffin

Western Blotting

Product Specific References

References for TGF Beta antibody

  1. Polichnowski, A.J. et al. (2014) Severe renal mass reduction impairs recovery and promotes fibrosis after AKI.
    J Am Soc Nephrol. 25 (7): 1496-507.
  2. Moore, L.B. et al. (2015) Loss of monocyte chemoattractant protein-1 alters macrophage polarization and reduces NFκB activation in the foreign body response.
    Acta Biomater. 11: 37-47.
  3. Geng, H. et al. (2012) Lysophosphatidic acid increases proximal tubule cell secretion of profibrotic cytokines PDGF-B and CTGF through LPA2- and Gαq-mediated Rho and αvβ6 integrin-dependent activation of TGF-β.
    Am J Pathol. 181: 1236-49.
  4. Tark, K.C. et al. (2015) Effects of ginsenoside Rb1 on hypertrophic scar remodeling in rabbit model.
    Eur J Pharmacol. 750C: 151-59.