NogoA antibody

100% Secure


Rabbit anti Human NogoA (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1799 P* WB 0.1 mg
Rabbit anti Human NOGOA antibody recognizes human Reticulon-4 (RTN4), also known as NogoA, neurite outgrowth inhibitor, Foocen, Neuroendocrine-specific protein, Neuroendocrine-specific protein C homolog, Reticulon-5 or RTN-x. NogoA is a 1192 amino acid multi pass transmembrane protein associated with the endoplasmic reticulum, a member of a family of integral membrane proteins termed reticulons. Six isoforms of NogoA can be generated by alternative splicing, the canonical isoform 1 is predominantly expressed in the brain and testis with weaker expression in the heart and skeletal muscle.

Reticulons are involved in various neurodegenerative diseases such as Amyotrophic lateral sclerosis,and multiple sclerosis (Chiurchiù et al. 2014). Reticulon proteins have been demonstrated to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor (Niederöst et al. 2002). NogoA is highly expressed in oligodendrocytes in the white matter of the CNS (Kuhlmann et al. 2008). Blocking NogoA activity with antibodies or other factors results in improved long distance axonal regeneration and functional recovery in experimental CNS lesion models (Schwab 2004).

NOGOA has a predicted molecular weight of 130kDa however, despite its predicted molecular weight, NogoA typically migrates at ~180kDa in an SDS-PAGE. Rabbit anti human NOGOA antibody is expected to recognize all isoforms of NogoA.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to human NogoA were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
Synthetic peptide sequence corresponding to a 23 amino acid sequence from near the carboxy terminus of Human NogoA
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9NQC3 Related reagents
Entrez Gene
RTN4 Related reagents
Regulatory
For research purposes only

Applications of NogoA antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 2.5ug/ml
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Mouse brain
Western Blotting
AHP1799 detects a band of approximately 180 kDa in Mouse Brain tissue lysate.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A 500 ml P
Antigen Retrieval Buffer, pH8.0 BUF025C 50 ml P
TidyBlot Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
Histar Detection System STAR3000A 50 Tests C P

Product Specific References

References for NogoA antibody

  1. Dann, A. et al. (2011) Cytosolic RIG-I-like helicases act as negative regulators of sterile inflammation in the CNS.
    Nat Neurosci. 15: 98-106.
  2. Gerondopoulos, A. et al. (2014) Rab18 and a Rab18 GEF complex are required for normal ER structure.
    J Cell Biol. 205 (5): 707-20.

Further Reading

  1. Yan, R. et al. (2006) Reticulon proteins: emerging players in neurodegenerative diseases.
    Cell Mol Life Sci. 63 (7-8): 877-89.
  2. Schweigreiter, R. & Bandtlow, C.E. (2006) Nogo in the injured spinal cord.
    J Neurotrauma. 23 (3-4): 384-96.
  3. Dupuis, L. et al. (2002) Nogo provides a molecular marker for diagnosis of amyotrophic lateral sclerosis.
    Neurobiol Dis. 10 (3): 358-65.
  4. Chen, M.S. et al. (2000) Nogo-A is a myelin-associated neurite outgrowth inhibitor and an antigen for monoclonal antibody IN-1.
    Nature. 403 (6768): 434-9.