CD49e antibody
Rabbit anti CD49e antibody recognizes CD49 antigen-like family member E, also known as integrin alpha-5 (ITGA5), integrin alpha-F and VLA-5. It belongs to the family of cell surface receptors and mediates signal transduction (Liu et al. 2000). CD49e was shown to regulate proliferation and adipogenic differentiation of human adipose derived stem cells (Morandi et al. 2016). MicroRNA-205 (miR-205) down-regulates CD49e and this impairs triple negative breast cancer cell metastatic traits by inhibiting the Src/Vav2/Rac1 pathway (Xiao et al. 2018).
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Rat Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to CD49e was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide
50% Glycerol - Immunogen
- Recombinant fusion protein containing a sequence corresponding to amino acids 750-1000 of human CD49e
Storage Information
- Storage
- Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- P08648
- Entrez Gene
- ITGA5
- GO Terms
- GO:0001525 angiogenesis
- GO:0001726 ruffle
- GO:0007596 blood coagulation
- GO:0004872 receptor activity
- GO:0005161 platelet-derived growth factor receptor binding
- GO:0008305 integrin complex
- GO:0007229 integrin-mediated signaling pathway
- GO:0007411 axon guidance
- GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
- GO:0035313 wound healing, spreading of epidermal cells
- GO:0043184 vascular endothelial growth factor receptor 2 binding
- GO:0044419 interspecies interaction between organisms
- GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
- GO:0050900 leukocyte migration
- GO:0071062 alphav-beta3 integrin-vitronectin complex
- Regulatory
- For research purposes only
Applications of CD49e antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Western Blotting | 1/500 | 1/2000 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Western Blotting
- Rabbit anti CD49e antibody detects a band of approximately 135 kDa in various cell and tissue lysates under reducing conditions
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Secondary Antibodies Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
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