CD49e antibody

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Anti CD49e Antibody gallery image 1 — Western blot analysis of NIH/3T3, HT1080 cell lysates and rat heart tissue lysate using Rabbit anti CD49e antibody at a 1/1000 dilution. 3% non-fat dry milk was used for blocking

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Rabbit anti CD49e

Product Type: Polyclonal Antibody
Isotype: Polyclonal IgG
Specificity: CD49e

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AHP2724 Datasheet Datasheet SDS SDS	WB	50 µl		Log in	Get Quote
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Rabbit anti CD49e antibody recognizes CD49 antigen-like family member E, also known as integrin alpha-5 (ITGA5), integrin alpha-F and VLA-5. It belongs to the family of cell surface receptors and mediates signal transduction (Liu et al. 2000). CD49e was shown to regulate proliferation and adipogenic differentiation of human adipose derived stem cells (Morandi et al. 2016). MicroRNA-205 (miR-205) down-regulates CD49e and this impairs triple negative breast cancer cell metastatic traits by inhibiting the Src/Vav2/Rac1 pathway (Xiao et al. 2018).

Target Species: Human
Species Cross-Reactivity: Target Species Cross Reactivity

Rat

Mouse; N.B. Antibody reactivity and working conditions may vary between species.
Product Form: Purified IgG - liquid
Antiserum Preparation: Antiserum to CD49e was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.02% Sodium Azide
50% Glycerol
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 750-1000 of human CD49e
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch

Target Species	Cross Reactivity
Rat
Mouse

Store at -20^oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Western Blotting		1/500	1/2000

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Western Blotting: Rabbit anti CD49e antibody detects a band of approximately 135 kDa in various cell and tissue lysates under reducing conditions

Description	Product Code	Applications	Pack Size	Your Price
TidyBlot Western Blot Detection Reagent:HRP	STAR209P	WB*	0.5 ml	Log in	Get Quote
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Description	TidyBlot Western Blot Detection Reagent:HRP

UniProt: P08648
Entrez Gene: ITGA5
GO Terms: GO:0001525 angiogenesis; GO:0001726 ruffle; GO:0007596 blood coagulation; GO:0004872 receptor activity; GO:0005161 platelet-derived growth factor receptor binding; GO:0008305 integrin complex; GO:0007229 integrin-mediated signaling pathway; GO:0007411 axon guidance; GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway; View More GO Terms; GO:0035313 wound healing, spreading of epidermal cells; GO:0043184 vascular endothelial growth factor receptor 2 binding; GO:0044419 interspecies interaction between organisms; GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation; GO:0050900 leukocyte migration; GO:0071062 alphav-beta3 integrin-vitronectin complex