CD49e antibody

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Rabbit anti CD49e

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product Code Applications Pack Size List Price Quantity
AHP2724
Datasheet
WB
SDS
50 µl loader

Rabbit anti CD49e antibody recognizes CD49 antigen-like family member E, also known as integrin alpha-5 (ITGA5), integrin alpha-F and VLA-5. It belongs to the family of cell surface receptors and mediates signal transduction (Liu et al. 2000). CD49e was shown to regulate proliferation and adipogenic differentiation of human adipose derived stem cells (Morandi et al. 2016). MicroRNA-205 (miR-205) down-regulates CD49e and this impairs triple negative breast cancer cell metastatic traits by inhibiting the Src/Vav2/Rac1 pathway (Xiao et al. 2018).

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Rat
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to CD49e was raised by repeated immunization of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide
50% Glycerol
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 750-1000 of human CD49e

Storage Information

Storage
Store at -20oC
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee
12 months from date of despatch

More Information

UniProt
P08648
Entrez Gene
ITGA5
GO Terms
GO:0001525 angiogenesis
GO:0001726 ruffle
GO:0007596 blood coagulation
GO:0004872 receptor activity
GO:0005161 platelet-derived growth factor receptor binding
GO:0008305 integrin complex
GO:0007229 integrin-mediated signaling pathway
GO:0007411 axon guidance
GO:0030949 positive regulation of vascular endothelial growth factor receptor signaling pathway
GO:0035313 wound healing, spreading of epidermal cells
GO:0043184 vascular endothelial growth factor receptor 2 binding
GO:0044419 interspecies interaction between organisms
GO:0050731 positive regulation of peptidyl-tyrosine phosphorylation
GO:0050900 leukocyte migration
GO:0071062 alphav-beta3 integrin-vitronectin complex
Regulatory
For research purposes only

Applications of CD49e antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Western Blotting 1/500 1/2000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Western Blotting
Rabbit anti CD49e antibody detects a band of approximately 135 kDa in various cell and tissue lysates under reducing conditions

Secondary Antibodies Available

Description Product Code Applications Pack Size List Price Quantity
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml