CD273 antibody

Rabbit anti Human CD273

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CD273

Product Code Applications Pack Size List Price Your Price Qty
AHP1704
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P * WB 0.1 mg loader
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Rabbit anti Human CD273 antibodyrecognizes human CD273, also known as B7-DC and Programmed Death Ligand 2 (PD-L2). CD273 is a single-pass type 1 membrane protein belonging to the immunoglobulin receptor superfamily, and is a ligand for CD279 (PD-1).

CD273 is highly expressed in the tissues of the heart, liver, lung, placenta and pancreas. It is expressed in dendritic cells, and its expression can be induced on inflammatory macrophages when activated by interleukin 4 or Th2 cells.

CD273 functions to inhibit T-cell proliferation by blocking cell cycle progression and cytokine production, and is particularly important in the maintenance of self tolerance.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Human CD273 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to a 16 amino acid sequence from near the centre of Human CD273.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 2.5ug/ml
Western Blotting 0.5 1.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Mouse brain tissue
Western Blotting
AHP1704 detects a band of approximately 30kDa in Raji cell lysate

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
List Price Your Price
Description Antigen Retrieval Buffer, pH8.0
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
List Price Your Price
Description TidyBlot Western Blot Detection Reagent:HRP

Further Reading

  1. Zhang, Y. et al. (2006) Regulation of T cell activation and tolerance by PDL2.
    Proc Natl Acad Sci U S A. 103 (31): 11695-700.
  2. LaGier, A.J. & Pober, J.S. (2006) Immune accessory functions of human endothelial cells are modulated by overexpression of B7-H1 (PDL1).
    Hum Immunol. 67 (8): 568-78.
  3. Ishida, Y. et al. (1992) Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death.
    EMBO J. 11 (11): 3887-95.
  4. Holling, T.M. et al. (2004) Function and regulation of MHC class II molecules in T-lymphocytes: of mice and men.
    Hum Immunol. 65 (4): 282-90.

Synonyms
PD-L2
RRID
AB_2162165
UniProt
Q9BQ51
Entrez Gene
PDCD1LG2
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005576 extracellular region
GO:0004872 receptor activity
GO:0006955 immune response
GO:0012505 endomembrane system
GO:0031295 T cell costimulation
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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