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CD234 antibody

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Anti Human CD234 (N-Terminal) Antibody thumbnail image 1 Anti Human CD234 (N-Terminal) Antibody thumbnail image 2
Anti Human CD234 (N-Terminal) Antibody gallery image 1
Western blot analysis of whole cell lysate from PC-3 human prostate cancer cells probed with Rabbit anti Human CD234 (AHP1708) at 0.5(A), 1(B) and 2(C) ug/ml.
Anti Human CD234 (N-Terminal) Antibody gallery image 2
Immunohistochemical staining of human brain with Rabbit anti Human CD234 (AHP1708).

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WB P

Rabbit anti Human CD234 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
CD234
Region
(N-TERMINAL)

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Product Code Applications Pack Size List Price Your Price Qty
AHP1708
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
P * WB 0.1 mg loader
List Price Your Price
loader

Rabbit anti Human CD234 antibody detects CD234, also known as DARC, a multi-pass membrane protein belonging to the G protein coupled Duffy family. CD234 is a non-specific receptor for a number of cytokines including IL-8, GRO, RANTES, MCP-1 and TARC.

CD234 also functions as the receptor for the malaria parasites Plasmodium vivax and P. knowlesi. CD234 is also responsible for the Duffy blood group system where a substitution at position 42 of either Gly-42 or Asp-42 results in Fy(A) or Fy(B).

CD234 is present in adult kidney, adult spleen, bone marrow and fetal liver. In particular, it is expressed along post capillary venules throughout the body, except in the adult liver. Erythroid cells and post capillary venule endothelium are the principle tissues expressing duffy. Fy(-A-B) individuals do not express duffy in the bone marrow, however they do, in postcapillary venule endothelium. It has been demonstrated that CD234 exists in multiple isoforms.

Target Species
Human
Product Form
Purified IgG - liquid
Antiserum Preparation
Antisera to Human CD234 were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
A peptide corresponding to a 18 amino acid sequence from near the amino terminus of Human CD234.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 5.0ug/ml
Western Blotting 1.0 2.0ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human brain tissue
Western Blotting
AHP1708 detects a band of approximately 16kDa in PC-3 cell lysate

Description Product Code Applications Pack Size List Price Your Price Quantity
Antigen Retrieval Buffer, pH8.0 BUF025A P 500 ml
List Price Your Price
Description Antigen Retrieval Buffer, pH8.0
TidyBlot Western Blot Detection Reagent:HRP STAR209P WB* 0.5 ml
List Price Your Price
Description TidyBlot Western Blot Detection Reagent:HRP

Further Reading

  1. Gardner, L. et al. (2004) The human Duffy antigen binds selected inflammatory but not homeostatic chemokines.
    Biochem Biophys Res Commun. 321 (2): 306-12.
  2. Chaudhuri, A. et al. (1993) Cloning of glycoprotein D cDNA, which encodes the major subunit of the Duffy blood group system and the receptor for the Plasmodium vivax malaria parasite.
    Proc Natl Acad Sci U S A. 90 (22): 10793-7.
  3. Gil, M.L. et al. (1992) A member of the tetra spans transmembrane protein superfamily is recognized by a monoclonal antibody raised against an HLA class I-deficient, lymphokine-activated killer-susceptible, B lymphocyte line. Cloning and preliminary functional studies.
    J Immunol. 148 (9): 2826-33.

Synonyms
DARC
RRID
AB_2088607
UniProt
Q16570
Entrez Gene
DARC

AHP1708

070415

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