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C1q antibody

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Published customer image:
Sheep anti Human C1q antibody (AHP033) used to evaluate C1q expression by flow cytometry.
Image caption:
Effects of SAP on C1q Binding to S. pneumoniae Measured Using Flow Cytometry. (A–C) Proportion of ST2 (A), ST4 (B), and ST23F (C) S. pneumoniae bacteria positive for C1q after incubation in 90 μg/ml human C1q with or without addition of 10 or 50 μg/ml hSAP. (D) Example of flow cytometry histograms of C1q binding to ST4 S. pneumoniae after incubation in 90 μg/ml human C1q with or without addition of 50 μg/ml hSAP. (E) Proportion of S. pneumoniae positive for C1q after incubation in human serum with (black shading) or without (grey shading) addition of 50 μg/ml hSAP, using C1q-depleted serum (white shading) as a negative control. (F) Example of flow cytometry histograms of C1q binding to ST2 S. pneumoniae after incubation in human serum with or without addition of 50 μg/ml hSAP. For panels (A–C) and (E), error bars represent SDs, and p-values are calculated using 2-tailed t tests.

From: Yuste J, Botto M, Bottoms SE, Brown JS (2007)
Serum Amyloid P Aids Complement-Mediated Immunity to Streptococcus pneumoniae.
PLoS Pathog 3(9): e120.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Sheep anti Human C1q:FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
C1q

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Sheep anti Human C1q antibody recognizes human C1q, a sub-component of the C1 complement component.

Target Species
Human
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Antiserum Preparation
Antisera to human C1q were raised by repeated immunisation of sheep with highly purified antigen. Purified IgG was prepared from whole serum by ion exchange chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
<0.1% Sodium Azide (NaN3)
Immunogen
Human C1q, purified from plasma.
Approx. Protein Concentrations
IgG concentration 10 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/25 1/50
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

References for C1q antibody

  1. Hwang, H.Y. et al. (2008) Highly specific inhibition of C1q globular-head binding to human IgG: a novel approach to control and regulate the classical complement pathway using an engineered single chain antibody variable fragment.
    Mol Immunol. 45: 2570-80.
  2. Cragg, M.S. and Glennie, M.J. (2004) Antibody specificity controls in vivo effector mechanisms of anti-CD20 reagents.
    Blood. 103: 2738-43.
  3. Sárvári, M. et al. (2003) Inhibition of C1q-beta-amyloid binding protects hippocampal cells against complement mediated toxicity.
    J Neuroimmunol. 137: 12-8.
  4. Lewis, M.J. et al. (2008) The different effector function capabilities of the seven equine IgG subclasses have implications for vaccine strategies.
    Mol Immunol. 45: 818-27.
  5. Yuste, J. et al. (2007) Serum amyloid P aids complement-mediated immunity to Streptococcus pneumoniae.
    PLoS Pathog. 3: 1208-19.
  6. Tang, Z. et al. (2013) A human single-domain antibody elicits potent antitumor activity by targeting an epitope in mesothelin close to the cancer cell surface.
    Mol Cancer Ther. 12: 416-26.
  7. John, M. et al. (2000) Nephrotic syndrome in a patient with IgA deficiency-associated mesangioproliferative glomerulonephritis.
    Pathology. 32 (1): 56-8.
  8. Ramos-sevillano, E. et al. (2015) Pleiotropic effects of cell wall amidase LytA on Streptococcus pneumoniae sensitivity to the host immune response.
    Infect Immun. 83 (2): 591-603.
    9. View The Latest Product References

ELISA

Synonyms
Complement Component 1q
RRID
AB_321206
UniProt
P02747
P02745
P02746
Entrez Gene
C1QA
C1QB
C1QC
GO Terms
GO:0005602 complement component C1 complex
GO:0006958 complement activation, classical pathway
GO:0007267 cell-cell signaling
GO:0045087 innate immune response
GO:0005576 extracellular region
GO:0030853 negative regulation of granulocyte differentiation
GO:0045650 negative regulation of macrophage differentiation
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