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AMBRA1 antibody

Rabbit anti AMBRA1 (C-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
AMBRA1
Region
(C-TERMINAL)

Product Code Applications Pack Size List Price Your Price Qty
AHP1889
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
IF P * WB 0.1 mg loader
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Rabbit anti AMBRA1 (C-Terminal) antibody recognizes human Activating molecule in beclin-1-regulated autophagy (AMBRA1), also known as WDR94.

AMBRA1 is involved in the regulation of autophagy. Research indicates that it controls protein turnover during neuronal development and in regulating normal cell survival and proliferation.

At least 5 isoforms of AMBRA have been identified.

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
Rat
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human AMBRA1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to a 15 amino acid sequence from near the carboxy terminus of human AMBRA1.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 20ug/ml
Immunohistology - Paraffin 1 5ug/ml
Western Blotting 1 2ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human brain

Synonyms
WDR94
RRID
AB_2273951
UniProt
Q9C0C7
Entrez Gene
AMBRA1
GO Terms
GO:0005776 autophagic vacuole
GO:0006914 autophagy
GO:0007399 nervous system development
GO:0030154 cell differentiation
GO:0031410 cytoplasmic vesicle

AHP1889

230315

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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