AMBRA1 antibody
Rabbit anti AMBRA1 (N-Terminal) antibody recognizes human Activating molecule in beclin-1-regulated autophagy (AMBRA1), also known as WDR94.
AMBRA1 is involved in the regulation of autophagy. Research indicates that it controls protein turnover during neuronal development and in regulating normal cell survival and proliferation.
At least 5 isoforms of AMBRA have been identified.
AMBRA1 is involved in the regulation of autophagy. Research indicates that it controls protein turnover during neuronal development and in regulating normal cell survival and proliferation.
At least 5 isoforms of AMBRA have been identified.
Product Details
- Target Species
- Human
- Species Cross-Reactivity
-
Target Species Cross Reactivity Mouse - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Antiserum Preparation
- Antiserum to human AMBRA1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.02% Sodium Azide (NaN3)
- Immunogen
- Synthetic peptide corresponding to an 18 amino acid sequence from the amino terminus of human AMBRA1.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
Storage Information
- Storage
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. - Guarantee
- 12 months from date of despatch
More Information
- UniProt
- Q9C0C7
- Entrez Gene
- AMBRA1
- GO Terms
- GO:0005776 autophagic vacuole
- GO:0006914 autophagy
- GO:0007399 nervous system development
- GO:0030154 cell differentiation
- GO:0031410 cytoplasmic vesicle
- Regulatory
- For research purposes only
Applications of AMBRA1 antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Paraffin 1 | 5ug/ml | ||
| Western Blotting | 2 | 4ug/ml |
- 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Histology Positive Control Tissue
- Human brain
- Western Blotting
- AHP1882 detects a band of approximately 160kDa in rat brain cell lysate.
Copyright © 2021 Bio-Rad Antibodies (formerly AbD Serotec)
Useful Reagents Available
| Description | Product Code | Applications | Pack Size | List Price | Quantity |
|---|---|---|---|---|---|
| TidyBlot Western Blot Detection Reagent:HRP | STAR209P | WB* | 0.5 ml |
Fluorescent Spectraviewer
Watch the Tool Tutorial Video ▸How to Use the Spectraviewer
Watch the Tool Tutorial Video ▸- Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
- Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
- Select the lasers and filters you wish to include
- Select combined or multi-laser view to visualize the spectra
