AMBRA1 antibody

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Rabbit anti AMBRA1 (N-Terminal)

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
AHP1882 P* WB 0.1 mg
Rabbit anti AMBRA1 (N-Terminal) antibody recognizes human Activating molecule in beclin-1-regulated autophagy (AMBRA1), also known as WDR94.

AMBRA1 is involved in the regulation of autophagy. Research indicates that it controls protein turnover during neuronal development and in regulating normal cell survival and proliferation.

At least 5 isoforms of AMBRA have been identified.

Product Details

Target Species
Human
Species Cross-Reactivity
Target SpeciesCross Reactivity
Mouse
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Antiserum Preparation
Antiserum to human AMBRA1 was raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG was prepared from whole serum by affinity chromatography.
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.02% Sodium Azide (NaN3)
Immunogen
Synthetic peptide corresponding to an 18 amino acid sequence from the amino terminus of human AMBRA1.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
Q9C0C7 Related reagents
Entrez Gene
AMBRA1 Related reagents
GO Terms
GO:0005776 autophagic vacuole
GO:0006914 autophagy
GO:0007399 nervous system development
GO:0030154 cell differentiation
GO:0031410 cytoplasmic vesicle
Regulatory
For research purposes only

Applications of AMBRA1 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Paraffin 1 5ug/ml
Western Blotting 2 4ug/ml
  1. 1This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Histology Positive Control Tissue
Human brain
Western Blotting
AHP1882 detects a band of approximately 160kDa in rat brain cell lysate.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
TidyBlot™ Western Blot Detection Reagent:HRP STAR209P 0.5 ml WB*
Histar Detection System STAR3000A 50 Tests C P