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Escherichia coli antibody

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Anti Escherichia coli Antibody thumbnail image 1 Anti Escherichia coli Antibody thumbnail image 2 Anti Escherichia coli Antibody thumbnail image 3 Anti Escherichia coli Antibody thumbnail image 4
Anti Escherichia coli Antibody gallery image 1
Published customer image:
Rabbit anti Escherichia coli. antibody (4329-4906) used to label bacterium by atomic force microscopy.
Image caption:
AFM scan of E. coli K-12 specifically bound on the cover slip modified by APTES and antibody Bio-Rad 4329-4906. The “Error” signal is shown. (A) Overview and (B) detail of a single cell.

From: Farka, Z et al.Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM. Sensors 2015, 15, 79-92.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Escherichia coli Antibody gallery image 2
Published customer image:
Rabbit anti Escherichia coli. antibody (4329-4906) used as an antibody reagent in an E. coli biosensor.
Image caption:
Bacterial adsorption on the Ab-coated sensor surfaces and the detection limit of OWLS. (A) Sensogram of a complete experiment, including the in situ coating procedures and subsequent bacteria adsorption for protein A-based immobilization and PAcrAM-P blocking. (B) E. coli adsorption on polyclonal Ab-coated surfaces using PAcrAM-P blocking; a 109 cells/mL concentration was employed. The signals with statistics are clearly distinguishable from the relevant control signal (full layer without Ab). (C) OWLS signal for a series of bacterial concentrations using protein A-based immobilization with PAcrAM-P blocking..

From: Farkas, E.; Tarr, R.; Gerecsei, T.; Saftics, A.; Kovács, K.D.; Stercz, B.; Domokos, J.; Peter, B.; Kurunczi, S.; Szekacs, I.; Bonyár, A.; Bányai, A.; Fürjes, P.; Ruszkai-Szaniszló, S.; Varga, M.; Szabó, B.; Ostorházi, E.; Szabó, D.; Horvath, R.
Development and In-Depth Characterization of Bacteria Repellent and Bacteria Adhesive Antibody-Coated Surfaces Using Optical Waveguide Biosensing.
Biosensors 2022, 12, 56.
doi: 10.3390/bios12020056
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Escherichia coli Antibody gallery image 3
Published customer image:
Rabbit anti Escherichia coli. antibody (4329-4906) used as an antibody reagent in an E. coli biosensor.
Image caption:
(A) Testing the binding abilities of different polyclonal Abs with an in-house ELISA test with different amounts of intact E. coli cells as antigen. Black(Rad): Bio-Rad 4329-4906, white(Blue): Thermo Fisher PA1-7213, grey(Green): Thermo Fisher PA1-73032. (B) The binding ability of a monoclonal Ab (Bio-Rad OBT0749) with an in-house ELISA test with different amounts of intact E. coli cells as antigen. For detection, biotinylated polyclonal Ab (Bio-Rad 4329-4906) was used. The inset shows the schematic drawing of the assay.

From: Farkas, E.; Tarr, R.; Gerecsei, T.; Saftics, A.; Kovács, K.D.; Stercz, B.; Domokos, J.; Peter, B.; Kurunczi, S.; Szekacs, I.; Bonyár, A.; Bányai, A.; Fürjes, P.; Ruszkai-Szaniszló, S.; Varga, M.; Szabó, B.; Ostorházi, E.; Szabó, D.; Horvath, R.
Development and In-Depth Characterization of Bacteria Repellent and Bacteria Adhesive Antibody-Coated Surfaces Using Optical Waveguide Biosensing.
Biosensors 2022, 12, 56.
doi: 10.3390/bios12020056
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Escherichia coli Antibody gallery image 4
Published customer image:
Rabbit anti Escherichia coli. antibody (4329-4906) used as an antibody reagent in an E. coli biosensor.
Image caption:
Typical OWLS kinetic curves of Ab deposition and their corresponding fits are shown. (A) Polyclonal Ab (Bio-Rad rabbit 4329-4906 on protein A surface). (B) Monoclonal Ab (Bio-Rad mouse OBT0749 on protein A surface). The smaller adsorbed surface mass density and slower adsorption are clearly visible in the case of the monoclonal Ab..From: Farkas, E.; Tarr, R.; Gerecsei, T.; Saftics, A.; Kovács, K.D.; Stercz, B.; Domokos, J.; Peter, B.; Kurunczi, S.; Szekacs, I.; Bonyár, A.; Bányai, A.; Fürjes, P.; Ruszkai-Szaniszló, S.; Varga, M.; Szabó, B.; Ostorházi, E.; Szabó, D.; Horvath, R.
Development and In-Depth Characterization of Bacteria Repellent and Bacteria Adhesive Antibody-Coated Surfaces Using Optical Waveguide Biosensing.
Biosensors 2022, 12, 56.
doi: 10.3390/bios12020056
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Rabbit anti Escherichia coli:FITC

Product Type
Polyclonal Antibody
Isotype
Polyclonal IgG
Specificity
Escherichia coli

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Product Code Applications Pack Size List Price Your Price Qty
4329-4916
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Rabbit anti Escherichia coli antibody recognizes Escherichia coli and is broadly reactive with all somatic and capsular (O and K) antigenic serotypes. The somatic O antigens are composed of lipopolysaccharide complexes which form part of the cell wall structure of E. coli whilst the capsular K antigens are mainly composed of acidic polysaccharide.

This antibody will remove E.coli proteins from recombinant preparations. Rabbit anti Escherichia coli antibody has not been absorbed and may cross-react with related enterobacteriaceae. Rabbit anti Escherichia coli antibody has been used in ELISA with serotypes O157:H7, O20, O125, 055, 0111 and K12.

Target Species
Bacterial
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.1%Sodium Azide (NaN3)
1%Bovine Serum Albumin
Immunogen
A mixture of all antigenic serotypes.
Approx. Protein Concentrations
IgG concentration 5.0 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunofluorescence 1/10 1/50
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

References for Escherichia coli antibody

  1. Tian, B. et al. (2015) Blu-ray optomagnetic measurement based competitive immunoassay for Salmonella detection.
    Biosens Bioelectron. 77: 32-9.
  2. Skladal, P. et al. (2013) Electrochemical immunosensors for detection of microorganisms
    Int. J. Electrochem. Sci., 8 1635 - 49
  3. Su, W.H. et al. (2013) Development of a chip-based multiplexed immunoassay using liposomal nanovesicles and its application in the detection of pathogens causing female lower genital tract infections.
    Taiwan J Obstet Gynecol. 52: 25-32.
  4. Ho, T.Y. et al. (2013) Development of a novel bead-based 96-well filtration plate competitive immunoassay for the detection of Gentamycin.
    Biosens Bioelectron. 49: 126-32.
  5. Ong, L.C. et al. (2014) Bacterial imaging with photostable upconversion fluorescent nanoparticles.
    Biomaterials. 35 (9): 2987-98.
  6. Pivetal, J. et al. (2014) Micro-magnet arrays for specific single bacterial cell positioning
    J. Magnetism and magnetic Materials 380 72-7.
  7. Farka, Z. et al. (2014) Rapid Detection of Microorganisms Based on Active and Passive Modes of QCM
    Sensors. 15 (1): 79-92.
  8. Dayam RM et al. (2015) The Phosphoinositide-Gated Lysosomal Ca(2+) Channel, TRPML1, Is Required for Phagosome Maturation.
    Traffic. 16 (9): 1010-26.
    9. View The Latest Product References
  9. Farka, Z. et al. (2015) Quartz crystal microbalance biosensor for rapid detection of aerosolized microorganisms.
    Proc. SPIE 9455, Chemical, Biological, Radiological, Nuclear, and Explosives (CBRNE) Sensing XVI, 945507
  10. Bhokisham, N. et al. (2016) Modular construction of multi-subunit protein complexes using engineered tags and microbial transglutaminase.
    Metab Eng. 38: 1-9.
  11. Kovář, D. et al. (2014) Detection of aerosolized biological agents using the piezoelectric immunosensor.
    Anal Chem. 86 (17): 8680-6.
  12. VanGerven, N. et al. (2014) Secretion and functional display of fusion proteins through the curli biogenesis pathway.
    Mol Microbiol. 91 (5): 1022-35.
  13. Rodrigues, D.M.C. et al. (2017) Sensitivity Analysis of Different Shapes of a Plastic Optical Fiber-Based Immunosensor for Escherichia coli: Simulation and Experimental Results.
    Sensors (Basel). 17 (12) Dec 19 [Epub ahead of print].

RRID
AB_616821

4329-4916

148888 149790 160463 165223 165922

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