SPYTAG3 Peptide
A microtiter plate was coated overnight with the monovalent Fab Human Anti-Ocrelizumab Antibody, clone AbD50651ad in SpyTag2 format at a concentration of 1 µg/ml. After washing and blocking with PBST + 5% BSA, a solution of PBST with (green) or without (red) Ocrelizumab at 2 µg/ml was added. Detection was performed using a monovalent Fab Human anti-Ocrelizumab Antibody coupled to the BiSpyCatcher:HRP, clone AbD50989pap at a concentration of 0.5 µg/ml in HISPEC Assay Diluent (BUF049A) with or without addition of SpyTag3 peptide, followed by QuantaBlu Fluorogenic Peroxidase Substrate. Data are shown as the mean of three measurements.
SpyTag3 Peptide
- Product Type
- Blocking Peptide
- Specificity
- SPYTAG3 Peptide
Antibody Quality and Performance Guarantee
| The SpyTag3 peptide is provided as a blocking peptide for use with antibodies ligated to SpyCatcher, BiCatcher and FcCatcher products. SpyTag3 peptide binds to excess SpyCatcher sites that have not reacted with an antibody during ligation. The blocking peptide will bind to SpyCatcher versions 1, 2 and 3. A recombinant antibody or any other protein with a SpyTag can be ligated to one of our many prefabricated SpyCatcher protein modules. The reaction between SpyTag and SpyCatcher requires only mixing and a short incubation. If the ligation reaction mixture contains an excess of the SpyCatcher partner, unreacted SpyCatcher sites remaining in the reaction mixture could interfere with other assay components and generate background signal. This can be prevented by the addition of SpyTag3 peptide to the final coupled antibody or protein preparation to block unreacted SpyCatcher sites. It is recommended to use a 2.5-fold molar excess of SpyTag3 peptide over SpyCatcher sites, i.e. 2.5-fold excess for SpyCatcher-coupled antibodies and 5-fold excess for BiCatcher- and FcCatcher-coupled antibodies and incubate for at least 5 minutes. Visit bio-rad-antibodies.com/SpyCatcher for further information. |
- Target Species
- Protein/peptide tag
- Product Form
- Purified peptide - liquid
- Source
- Chemical synthesis
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- None present
- Purity
- >90% by RP-HPLC
- Approx. Protein Concentrations
- 1 mM (1.93 mg/ml)
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- Sold under license of patent and patent applications, including but not limited to the international PCT application PCT/GB2018/051065, the European patent EP2534484B1, UK patent GB1706430.4, the US patents US9,547,003, US10,247,727, US patent application US16/274908 and corresponding patents
Store at -20°C only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunoassay |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| SpyCatcher2 | TZC001 | IY | 1 mg |
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| Description | SpyCatcher2 | ||||||
| SpyCatcher2:Biotin | TZC001B | IY | 1 mg |
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| List Price | Your Price | ||||||
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| Description | SpyCatcher2:Biotin | ||||||
| SpyCatcher2-CYS | TZC001CYS | IY | 1 mg |
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| Description | SpyCatcher2-CYS | ||||||
| BiSpyCatcher2 | TZC002 | IY | 1 mg |
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| Description | BiSpyCatcher2 | ||||||
| BiSpyCatcher2:Biotin | TZC002B | IY | 1 mg |
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| Description | BiSpyCatcher2:Biotin | ||||||
| BiSpyCatcher2-CYS | TZC002CYS | IY | 1 mg |
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| Description | BiSpyCatcher2-CYS | ||||||
| BiSpyCatcher2-CYS3 | TZC002CYS3 | IY | 1 mg |
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| Description | BiSpyCatcher2-CYS3 | ||||||
| BiSpyCatcher2:HRP | TZC002P | IY | 1 mg |
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| Description | BiSpyCatcher2:HRP | ||||||
| Human IgG1-FcSpyCatcher3 | TZC009 | IY | 1 mg |
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| List Price | Your Price | ||||||
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| Description | Human IgG1-FcSpyCatcher3 | ||||||
| Mouse IgG2a-FcSpyCatcher3 | TZC012 | IY | 0.5 mg |
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| Description | Mouse IgG2a-FcSpyCatcher3 | ||||||
| Rabbit IgG-FcSpyCatcher3 | TZC013 | IY | 0.5 mg |
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| Description | Rabbit IgG-FcSpyCatcher3 | ||||||
| Human IgG2-FcSpyCatcher3 | TZC016 | IY | 0.5 mg |
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| Description | Human IgG2-FcSpyCatcher3 | ||||||
| Human IgG3-FcSpyCatcher3 | TZC017 | IY | 0.5 mg |
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| Description | Human IgG3-FcSpyCatcher3 | ||||||
| Human IgG4-FcSpyCatcher3 | TZC018 | IY | 0.5 mg |
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| Description | Human IgG4-FcSpyCatcher3 | ||||||
| Human IgG4-Pro-FcSpyCatcher3 | TZC019 | IY | 0.5 mg |
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| Description | Human IgG4-Pro-FcSpyCatcher3 | ||||||
| Human IgA-FcSpyCatcher3 | TZC020 | IY | 0.5 mg |
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| Description | Human IgA-FcSpyCatcher3 | ||||||
| SpyCatcher3 | TZC025 | IY | 1 mg |
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| Description | SpyCatcher3 | ||||||
| SpyCatcher3-CYS | TZC025CYS | IY | 1 mg |
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| Description | SpyCatcher3-CYS | ||||||
Antibody Characterization Reference
-
Keeble, A.H. & Howarth, M. (2020) Power to the protein: enhancing and combining activities using the Spy toolbox.
Chem Sci. 11 (28): 7281-7291. -
Zakeri, B. et al. (2012) Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin.
Proc Natl Acad Sci U S A. 109 (12): E690-7.
BLP086
161775
163754
166671
168116
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