Mycoplasma Removal Agent

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Mycoplasma Removal Agent gallery image 1

The effect of 0.5ug/ml Mycoplasma Removal Agent (BUF035) added to the culture medium of mycoplasma-infected cells before (left) and after 1 week (right)

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  • Mycoplasma Removal Agent
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    Mycoplasma removal agent is used to remove mycoplasma from infected cell cultures. It shows strong anti-mycoplasma activity against many types of mycoplasma including Mycoplasma orale, M. arginini, M. hyorhinis and Acholeplasma laidlawii.
    • Product Type
      Accessory Reagent
    1 Formats Available
      Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
      BUF035TCdatasheet pdfdatasheet pdf5 ml
      BUF035
      Summary
      Secondary Antibodies
      Negative Isotype Controls
      Useful Reagents
      Positive Controls
      Histology Controls
      More Images
      References
      Reviews
      -
      • Intended Use
        MRA is very effective at removing mycoplasma from infected cell cultures. It shows strong anti-mycoplasma activity against many types of mycoplasma including Mycoplasma orale, M. arginini, M. hyorhinis and Acholeplasma laidlawii.

        MRA is also suitable for use after the removal of mycoplasma, to prevent recontamination of the culture with the original mycoplasma, at preventative doses.

        This product can also be used to prevent initial infection of cells in culture by mycoplasma.

        MRA is non toxic, and will not interfere with the viability or function of cells in culture. It should be emphasised that MRA should not be used as a substitute for good cell culture techniques.
      • Product Form
      • Reconstitution
      • Preparation
      • Preservative Stabilisers
      • Purity
      • Approx. Protein Concentrations
        50ug/ml
      • Reagents In The Kit
      • Preparing The Antibody
      • Test Principle
      • Buffer Solution
      • Storage
        Store at room temperature.
        This product should be stored undiluted.
        This product is photosensitive and should be protected from light.
      • Shelf Life
        5 years from date of despatch.
      • Acknowledgements
      • Regulatory
        For research purposes only
      • This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

      • Application NameYesNoMin DilutionMax Dilution
        Tissue Culture

      • Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
      • Technical Advice
      • Recommended Protocol
      • ELISA
      • Immunohistology
      • Histology Positive Control Tissue
      • Immunofluorescence
      • Western Blotting
      • Instructions For Use
        MRA is very easy to use, simply requiring incubation for a week after addition to cell cultures contaminated by mycoplasma.

        Indications for Use:

        1. Add MRA to cell cultures contaminated by mycoplasma at a concentration of 0.5 µg/ml and incubate for a week.

        2. For media replacement or culture transfer (passage), use a medium containing MRA at this same concentration.

        3. Transfer the cell cultures several times without MRA and confirm that regrowth of the contaminating mycoplasma has not occurred.

        If there is a concern about the presence of mycoplasma in serum or trypsin, MRA can be added to the media at a concentration of 0.5 µg/ml to prevent contamination of the cell cultures exposed to these products.

        N.B. The recommended concentration for use is 0.5 µg/ml. The MRA concentration may be raised up to 1 µg/ml only when the recommended concentration is ineffective in removing the mycoplasma.

        The cytotoxicity of MRA is low and cell toxicity is rare when used at the recommended concentration. For specific function of any cell, however, it is recommended that the retention of desired cellular characteristics be confirmed after treatment.
        Please follow this link to view Sample Data.

      Additional Mycoplasma Removal Agent Formats

      Formats Applications Sizes available
      Mycoplasma Removal Agent : Kit TC 5 ml
      • Copyright © 2016 Bio-Rad Antibodies (formerly AbD Serotec)

      Recommended Secondary Antibody

        Recommended Negative Isotype Control

          Useful Reagents

            DescriptionProduct CodePack SizeApplicationsList PriceQuantity
            Mouse Monoclonal Antibody Isotyping Test KitMMT110 TestsIS
            MMT1
            Rat Monoclonal Antibody Isotyping Test KitRMT110 TestsIS
            RMT1
            Proteus Protein A Mini Purification KitPUR00816 UnitsPP
            PUR008
            Proteus Protein G Mini Purification KitPUR01616 UnitsPP
            PUR016
            Proteus IMAC Mini Purification KitPUR02624 UnitsPP
            PUR026
            Proteus Protein A MIDI Purification KitPUR0034 UnitsPP
            PUR003
            Proteus Protein G MIDI Purification KitPUR0124 UnitsPP
            PUR012
            Proteus IMAC MIDI Purification KitPUR0218 UnitsPP
            PUR021

            Recommended Positive Controls

              Histology Controls

                References

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