DC012 is suitable for use as a negative control for the measurement of non-specific binding of mouse monoclonals of isotype IgG1 to human tissues in a dual labelling technique using FITC and R-Phycoerythrin fluorochromes.
Dual Colour combination consisting of FITC conjugated and RPE conjugated monoclonal antibodies mixed in optimal ratio - lyophilised.
Prior to reconstitution store at +4oC. Following reconstitution store at +4oC
This product should be stored undiluted.
DO NOT FREEZE. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
12 months from date of reconstitution.
For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Histology Positive Control Tissue
Instructions For Use
Additional Mouse IgG1/IgG1 Negative Control Antibody Formats
1. Steele, J. et al. (2002) Detection of CD4(+)- and CD8(+)- T-cell responses to human papillomavirus type 1 antigens expressed at various stages of the virus life cycle by using an enzyme-linked immunospot assay of gamma Interferon release. J. Virol. 76: 6027 - 6036.