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Yellow Fever Virus antibody | 2D12 (0G5)

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Mouse anti Yellow Fever virus antibody, clone 2D12 used for the detection of yellow fever envelope protein in infected dendritic cells by flow cytometry.
Image caption:
Monocyte derived dendritic cells (MoDCs) infection with dengue virus 2 (DENV2) and yellow fever (YF) 17DD. MoDCs were infected with DENV2 or YF17DD at equivalent multiplicities of infections (MOIs) [2.5 x 105 50% tissue culture infectious dose (TCID)/ml] or YF17DD at higher MOI (1.25 x 106 TCID50/ml) or mock infected. Cells were labelled with anti DENV or anti YF antibodies using triplicates for each peripheral blood mononuclear cell (PBMC) donor. A: flow cytometry patterns for viral antigens on gated MoDCs 24 h after viral infection. Histograms demonstrate cell distribution by Alexa Fluor-488 fluorescence intensity (FL1-H: level of fluorescence). Cells were cultured with mock (dark grey) or DENV2 or YF17DD (light grey) at concentrated (conc) or equivalent (equiv) doses; B: viral antigen detection kinetics on infected MoDcs (data from four PBMC donors) [hpi: hours post-infection; X axis: time after infection (hours); Y axis: percentage of viral antigen positive cells (Ag+)]; C: intracellular viral antigen detection 24h after infection representing 11 cell donors (horizontal lines: distribution mean; X axis: viral inoculum; Y axis: percentage of viral Ag+ cells. Asterisks mean: p<0.0001 in one way analysis of variance and Tukey&s multiple comparison test).

From: Gandini M, Reis SR, Torrentes-Carvalho A, Azeredo EL, Freire Mda S, Galler R, Kubelka CF.
Dengue-2 and yellow fever 17DD viruses infect human dendritic cells, resulting in an induction of activation markers, cytokines and chemokines and secretion of different TNF-α and IFN-α profiles.
Mem Inst Oswaldo Cruz. 2011 Aug;106(5):594-605.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.

Mouse anti Yellow Fever Virus

Product Type
Monoclonal Antibody
Clone
2D12 (0G5)
Isotype
IgG2a
Specificity
Yellow Fever Virus

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Mouse anti Yellow fever virus antibody, clone 2D12 recognizes the envelope protein of the wild (Asibi) and vaccine strains of yellow fever virus. Mouse anti Yellow fever virus antibody, clone 2D12 has been reported to have neutralizing activity against the Asibi strain (Schlessinger et al. 1984). No cross reactivity with other flaviviruses has been reported.

Target Species
Viral
Product Form
Ascites - Liquid
Preservative Stabilisers
None Present
Immunogen
17D strain of yellow fever virus
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

Store at -20oC only.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Functional Assays
Immunofluorescence
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

References for Yellow Fever Virus antibody

  1. Schlesinger, J.J. et al. (1983) Monoclonal antibodies distinguish between wild and vaccine strains of yellow fever virus by neutralization, hemagglutination inhibition, and immune precipitation of the virus envelope protein.
    Virology. 125 (1): 8-17.
  2. Schlesinger, J.J. & Brandriss, M.W. (1983) 17D yellow fever virus infection of P388D1 cells mediated by monoclonal antibodies: properties of the macrophage Fc receptor.
    J Gen Virol. 64 (Pt 6): 1255-62.
  3. Schlesinger, J.J. et al. (1984) Analysis of 17D yellow fever virus envelope protein epitopes using monoclonal antibodies.
    J Gen Virol. 65 ( Pt 10): 1637-44.
  4. Gandini, M. et al. (2011) Dengue-2 and yellow fever 17DD viruses infect human dendritic cells, resulting in an induction of activation markers, cytokines and chemokines and secretion of different TNF-α and IFN-α profiles.
    Mem Inst Oswaldo Cruz. 106 (5): 594-605.
  5. Monath, T.P. et al. (1986) Sensitive and specific monoclonal immunoassay for detecting yellow fever virus in laboratory and clinical specimens.
    J Clin Microbiol. 23 (1): 129-34.
  6. Brandriss, M.W. et al. (1986) Lethal 17D yellow fever encephalitis in mice. I. Passive protection by monoclonal antibodies to the envelope proteins of 17D yellow fever and dengue 2 viruses.
    J Gen Virol. 67 ( Pt 2): 229-34.
  7. Op De Beeck, A. et al. (2003) Role of the transmembrane domains of prM and E proteins in the formation of yellow fever virus envelope.
    J Virol. 77 (2): 813-20.
  8. Izurieta, R.O. et al. (2009) Anamnestic immune response to dengue and decreased severity of yellow Fever.
    J Glob Infect Dis. 1 (2): 111-6.
    9. View The Latest Product References
  9. Thonnon, J. et al. (1998) Re-emergence of yellow fever in Senegal in 1995.
    Am J Trop Med Hyg. 59 (1): 108-14.
  10. Thonnon, J.et al. (1998) Yellow fever outbreak in Kaffrine, Senegal 1996: epidemiological and entomological findings.
    Trop Med Int Health. 3 (11): 872-7.
  11. Op De Beeck, A. et al. (2004) The transmembrane domains of the prM and E proteins of yellow fever virus are endoplasmic reticulum localization signals.
    J Virol. 78 (22): 12591-602.
  12. Ciczora, Y. et al. (2010) Identification of a dominant endoplasmic reticulum-retention signal in yellow fever virus pre-membrane protein.
    J Gen Virol. 91 (Pt 2): 404-14.
  13. Vratskikh, O. et al. (2013) Dissection of antibody specificities induced by yellow fever vaccination.
    PLoS Pathog. 9 (6): e1003458.

Immunofluorescence

Immunoprecipitation

RRID
AB_619294

9801-8006

151920 180222

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