GM-CSF antibody | 8D8

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Mouse anti Sheep GM-CSF

Product Type
Monoclonal Antibody
Clone
8D8
Isotype
IgG1
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA1923 E WB 0.25 mg
Mouse anti Sheep GM-CSF antibody, clone 8D8 recognizes both recombinant and native ovine granulocyte / macrophage colony-stimulating factor (GM-CSF), also known as Colony stimulating factor (CSF).

GM-CSF is one of several cytokines that regulate the survival, growth and differentiation of haemopoietic cells. GM-CSF is secreted from a range of cells including macrophages, T-cells, endothelial cells and fibroblasts in response to antigen, LPS or mitogen stimulation.

Mouse anti Sheep GM-CSF antibody, clone 8D8 may be used as a capture antibody in ELISA assays with Mouse anti Sheep GM-CSF antibody, clone 3C2 (MCA1924) as a detection reagent for the evaluation of ovine GM-CSF.

Mouse anti Sheep GM-CSF antibody, clone 8D8 has been reported to neutralize the activity of both recombinant and native ovine GM-CSF (Entrican et al. 1996). Removal of the preservative is recommended prior to use in functional assays - Bio-Rad recommend the use of a Slide-A-Lyser® dialysis cassette for this purpose.

Product Details

Target Species
Sheep
Species Cross-Reactivity
Target SpeciesCross Reactivity
Bovine
Horse Weak
Goat Weak
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Carrier Free
Yes
Immunogen
CHO expressed recombinant ovine GM-CSF.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunised Balb/c mice were fused with cells of the NS0 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P28773 Related reagents
Entrez Gene
CSF2 Related reagents
GO Terms
GO:0005125 cytokine activity
GO:0005129 granulocyte macrophage colony-stimulating factor receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity
Acknowledgements
This reagent was evaluated at the Moredun Research Institute and The Roslin Institute at the University of Edinburgh as part of ‘The route to identification of immunological correlates of protection in ruminants’ Industrial Partnership Award funded by BBSRC (grant numbers BB/I019863/1; BB/I020519/1) with the support of Bio-Rad.
Regulatory
For research purposes only

Applications of GM-CSF antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5ug/ml
Western Blotting
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Rabbit F(ab')2 anti Mouse IgG:Biotin STAR11B 1 mg C E F P

Application Based External Images

ELISA

Product Specific References

References for GM-CSF antibody

  1. Entrican, G. et al. (1996) Development of a sandwich ELISA for ovine granulocyte/macrophage colony-stimulating factor.
    Vet Immunol Immunopathol. 50 (1-2): 105-15.
  2. Pedersen, L.G. et al. (2002) Identification of monoclonal antibodies that cross-react with cytokines from different animal species.
    Vet Immunol Immunopathol. 88 (3-4): 111-22.
  3. Scott, J.L. et al. (2007) Granulocyte-macrophage colony stimulating factor and interleukin-8 in the reproductive tract of ewes following oestrus and mating.
    Reprod Fertil Dev. 19: 585-93.
  4. John, H.A. et al. (1994) Generation of an ovine bone marrow-derived myelomonocyte-like cell line by retroviral-mediated transformation. Immunological characterization and the effect of cytokines and lipopolysaccharides.
    J Leukoc Biol. 55: 785-92.
  5. Deane, D. et al. (2000) Orf virus encodes a novel secreted protein inhibitor of granulocyte-macrophage colony-stimulating factor and interleukin-2.
    J Virol. 74: 1313-20.
  6. Schnabel, C.L. et al. (2013) Evaluation of the reactivity of commercially available monoclonal antibodies with equine cytokines
    Veterinary Immunology and Immunopathology. 156 (1-2): 1-19.