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GM-CSF antibody | 8D8

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E WB 0.25 mg loader
List Price Your Price

Mouse anti Sheep GM-CSF antibody, clone 8D8 recognizes both recombinant and native ovine granulocyte / macrophage colony-stimulating factor (GM-CSF), also known as Colony stimulating factor (CSF).

GM-CSF is one of several cytokines that regulate the survival, growth and differentiation of haemopoietic cells. GM-CSF is secreted from a range of cells including macrophages, T-cells, endothelial cells and fibroblasts in response to antigen, LPS or mitogen stimulation.

Mouse anti Sheep GM-CSF antibody, clone 8D8 may be used as a capture antibody in ELISA assays with Mouse anti Sheep GM-CSF antibody, clone 3C2 (MCA1924) as a detection reagent for the evaluation of ovine GM-CSF.

Mouse anti Sheep GM-CSF antibody, clone 8D8 has been reported to neutralize the activity of both recombinant and native ovine GM-CSF (Entrican et al. 1996). Removal of the preservative is recommended prior to use in functional assays.

Target Species
Species Cross-Reactivity
Target SpeciesCross Reactivity
Horse Weak
Goat Weak
N.B. Antibody reactivity and working conditions may vary between species.
Product Form
Purified IgG - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
Carrier Free
CHO expressed recombinant ovine GM-CSF.
Approx. Protein Concentrations
IgG concentration 1.0mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the NS0 myeloma cell line.
For research purposes only
12 months from date of despatch
This reagent was evaluated at the Moredun Research Institute and The Roslin Institute at the University of Edinburgh as part of ‘The route to identification of immunological correlates of protection in ruminants’ Industrial Partnership Award funded by BBSRC (grant numbers BB/I019863/1; BB/I020519/1) with the support of Bio-Rad.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 5ug/ml
Western Blotting
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
Rabbit F(ab')2 anti Mouse IgG:Biotin STAR11B C E F P 1 mg loader
List Price Your Price
Description Rabbit F(ab')2 anti Mouse IgG:Biotin

References for GM-CSF antibody

  1. John, H.A. et al. (1994) Generation of an ovine bone marrow-derived myelomonocyte-like cell line by retroviral-mediated transformation. Immunological characterization and the effect of cytokines and lipopolysaccharides.
    J Leukoc Biol. 55: 785-92.
  2. Entrican, G. et al. (1996) Development of a sandwich ELISA for ovine granulocyte/macrophage colony-stimulating factor.
    Vet Immunol Immunopathol. 50 (1-2): 105-15.
  3. Deane, D. et al. (2000) Orf virus encodes a novel secreted protein inhibitor of granulocyte-macrophage colony-stimulating factor and interleukin-2.
    J Virol. 74: 1313-20.
  4. Pedersen, L.G. et al. (2002) Identification of monoclonal antibodies that cross-react with cytokines from different animal species.
    Vet Immunol Immunopathol. 88 (3-4): 111-22.
  5. Scott, J.L. et al. (2007) Granulocyte-macrophage colony stimulating factor and interleukin-8 in the reproductive tract of ewes following oestrus and mating.
    Reprod Fertil Dev. 19: 585-93.
  6. Schnabel, C.L. et al. (2013) Evaluation of the reactivity of commercially available monoclonal antibodies with equine cytokines.
    Vet Immunol Immunopathol. 156 (1-2): 1-19.


Granulocyte Macrophage Colony Stimulating Factor
Entrez Gene
GO Terms
GO:0005125 cytokine activity
GO:0005129 granulocyte macrophage colony-stimulating factor receptor binding
GO:0005615 extracellular space
GO:0006955 immune response
GO:0008083 growth factor activity



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