GM-CSF antibody | 8D8
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Mouse anti Sheep GM-CSF
- Product Type
- Monoclonal Antibody
- Clone
- 8D8
- Isotype
- IgG1
- Specificity
- GM-CSF
Antibody Quality and Performance Guarantee
| Mouse anti Sheep GM-CSF antibody, clone 8D8 recognizes both recombinant and native ovine granulocyte / macrophage colony-stimulating factor (GM-CSF), also known as Colony stimulating factor (CSF). GM-CSF is one of several cytokines that regulate the survival, growth and differentiation of haemopoietic cells. GM-CSF is secreted from a range of cells including macrophages, T-cells, endothelial cells and fibroblasts in response to antigen, LPS or mitogen stimulation. Mouse anti Sheep GM-CSF antibody, clone 8D8 may be used as a capture antibody in ELISA assays with Mouse anti Sheep GM-CSF antibody, clone 3C2 (MCA1924) as a detection reagent for the evaluation of ovine GM-CSF. Mouse anti Sheep GM-CSF antibody, clone 8D8 has been reported to neutralize the activity of both recombinant and native ovine GM-CSF (Entrican et al. 1996). Removal of the preservative is recommended prior to use in functional assays. |
- Target Species
- Sheep
- Species Cross-Reactivity
-
Target Species Cross Reactivity Bovine Horse Weak Goat Weak - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- CHO expressed recombinant ovine GM-CSF.
- Approx. Protein Concentrations
- IgG concentration 1.0mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the NS0 myeloma cell line.
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This reagent was evaluated at the Moredun Research Institute and The Roslin Institute at the University of Edinburgh as part of ‘The route to identification of immunological correlates of protection in ruminants’ Industrial Partnership Award funded by BBSRC (grant numbers BB/I019863/1; BB/I020519/1) with the support of Bio-Rad.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
5ug/ml | |
| Western Blotting | |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Rabbit F(ab')2 anti Mouse IgG:Biotin | STAR11B | C E F P | 1 mg |
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| Description | Rabbit F(ab')2 anti Mouse IgG:Biotin | ||||||
References for GM-CSF antibody
-
Entrican, G. et al. (1996) Development of a sandwich ELISA for ovine granulocyte/macrophage colony-stimulating factor.
Vet Immunol Immunopathol. 50 (1-2): 105-15. -
Pedersen, L.G. et al. (2002) Identification of monoclonal antibodies that cross-react with cytokines from different animal species.
Vet Immunol Immunopathol. 88 (3-4): 111-22. -
Scott, J.L. et al. (2007) Granulocyte-macrophage colony stimulating factor and interleukin-8 in the reproductive tract of ewes following oestrus and mating.
Reprod Fertil Dev. 19: 585-93. -
John, H.A. et al. (1994) Generation of an ovine bone marrow-derived myelomonocyte-like cell line by retroviral-mediated transformation. Immunological characterization and the effect of cytokines and lipopolysaccharides.
J Leukoc Biol. 55: 785-92. -
Deane, D. et al. (2000) Orf virus encodes a novel secreted protein inhibitor of granulocyte-macrophage colony-stimulating factor and interleukin-2.
J Virol. 74: 1313-20. -
Schnabel, C.L. et al. (2013) Evaluation of the reactivity of commercially available monoclonal antibodies with equine cytokines.
Vet Immunol Immunopathol. 156 (1-2): 1-19.
- Synonyms
- Granulocyte Macrophage Colony Stimulating Factor
- RRID
- AB_10614177
- UniProt
- P28773
- Entrez Gene
- CSF2
- GO Terms
- GO:0005125 cytokine activity
- GO:0005129 granulocyte macrophage colony-stimulating factor receptor binding
- GO:0005615 extracellular space
- GO:0006955 immune response
- GO:0008083 growth factor activity
MCA1923
1609
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