Kappa Light Chain antibody | OX-12

Mouse anti Rat Kappa Light Chain:HRP

Product Type
Monoclonal Antibody
Clone
OX-12
Isotype
IgG2a
Specificity
Kappa Light Chain
Region
(IgKa PREFERENCE)

Product Code Applications Pack Size List Price Your Price Qty
MCA49P
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.5 mg loader
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loader
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Mouse anti Rat Kappa Light Chain antibody, clone OX-12 recognizes rat kappa chains, showing a marked preference for binding to the Igka allotype which is present in AGUS, AO, AUG, BN, LEW, LOU, PVG and WAG strains but not in ACI, DA, F344, MSU or SHR rat strains.

This product is routinely tested in flow cytometry on rat splenic lymphocytes.

Target Species
Rat
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Thiomersal
Immunogen
F(ab')2 fragment of rat IgG.
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Description Product Code Applications Pack Size List Price Your Price Quantity
TMB Core+ BUF062A E 100 ml
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Description TMB Core+
TMB Signal+ BUF054A E 100 ml
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Description TMB Signal+
TMB Core BUF056A E 100 ml
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Description TMB Core
AbGUARD® HRP Stabilizer Plus BUF052A C E P WB 100 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052B C E P WB 500 ml
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Description AbGUARD® HRP Stabilizer Plus
AbGUARD® HRP Stabilizer Plus BUF052C C E P WB 1000 ml
List Price Your Price
Description AbGUARD® HRP Stabilizer Plus

References for Kappa Light Chain antibody

  1. Hunt, S.V. & Fowler, M.H. (1981) A repopulation assay for B and T lymphocyte stem cells employing radiation chimaeras.
    Cell Tissue Kinet. 14 (4): 445-64.
  2. Bukovský, A. et al. (1984) Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy.
    Immunology. 52 (4): 631-40.
  3. Balogh, P. et al. (2002) Simultaneous blockade of Fc gamma receptors and indirect labeling of mouse lymphocytes by the selective detection of allotype-restricted epitopes on the kappa chain of rat monoclonal antibodies.
    Cytometry. 47 (2): 107-10.
  4. Lozano, F. et al. (1993) Affinity maturation leads to differential expression of multiple copies of a kappa light-chain transgene.
    Nature. 363 (6426): 271-3.
  5. Gehrs, B.C. et al. (1997) Alterations in the developing immune system of the F344 rat after perinatal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin: II. Effects on the pup and the adult.
    Toxicology. 122 (3): 229-40.
  6. Penny, M.J. et al. (1997) Role of T cells in the mediation of Heymann nephritis. ii. Identification of Th1 and cytotoxic cells in glomeruli.
    Kidney Int. 51 (4): 1059-68.
  7. Cisternas, P.A. & Armati, P.J. (2000) Immune system cell markers in the northern brown bandicoot, Isoodon macrourus.
    Dev Comp Immunol. 24 (8): 771-82.
  8. Pabst, R. et al. (2009) TLR2/6 stimulation of the rat lung: effects on lymphocyte subsets, natural killer cells and dendritic cells in different parts of the air-conducting compartments and at different ages.
    Immunology. 126 (1): 132-9.
  9. View The Latest Product References
  10. Pérez-cano, F.J. et al. (2007) Phenotypic and functional characteristics of rat spleen lymphocytes during suckling.
    Dev Comp Immunol. 31 (12): 1264-77.
  11. Mccormack, E. et al. (2010) Lentinan: hematopoietic, immunological, and efficacy studies in a syngeneic model of acute myeloid leukemia.
    Nutr Cancer. 62 (5): 574-83.
  12. Emara, A.M. & Draz, E.I. (2007) Immunotoxicological study of one of the most common over-the-counter pyrethroid insecticide products in Egypt.
    Inhal Toxicol. 19 (12): 997-1009.

RRID
AB_567002
UniProt
P01836
P01835
Entrez Gene
LOC500180
GO Terms
GO:0003823 antigen binding
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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