Mouse anti Rat Kappa Light Chain antibody, clone OX-12 recognizes rat kappa chains, showing a marked preference for binding to the Igka allotype which is present in AGUS, AO, AUG, BN, LEW, LOU, PVG and WAG strains but not in ACI, DA, F344, MSU or SHR rat strains.
This product is routinely tested in flow cytometry on rat splenic lymphocytes.
- Target Species
- Product Form
- Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
- Purified IgG prepared by affinity chromatography on Protein G
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- F(ab')2 fragment of rat IgG.
- Approx. Protein Concentrations
- IgG concentration 1.0 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Entrez Gene
- GO Terms
- For research purposes only
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Applications of Kappa Light Chain antibody
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
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Useful Reagents Available
Product Specific References
References for Kappa Light Chain antibody
Hunt, S.V. & Fowler, M.H. (1981) A repopulation assay for B and T lymphocyte stem cells employing radiation chimaeras.
Cell Tissue Kinet. 14 (4): 445-64.
Bukovský, A. et al. (1984) Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy.
Immunology. 52 (4): 631-40.
Balogh, P. et al. (2002) Simultaneous blockade of Fc gamma receptors and indirect labeling of mouse lymphocytes by the selective detection of allotype-restricted epitopes on the kappa chain of rat monoclonal antibodies.
Cytometry. 47 (2): 107-10.
Lozano, F. et al. (1993) Affinity maturation leads to differential expression of multiple copies of a kappa light-chain transgene.
Nature. 363 (6426): 271-3.
Gehrs, B.C. et al. (1997) Alterations in the developing immune system of the F344 rat after perinatal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin: II. Effects on the pup and the adult.
Toxicology. 122 (3): 229-40.
Penny, M.J. et al. (1997) Role of T cells in the mediation of Heymann nephritis. ii. Identification of Th1 and cytotoxic cells in glomeruli.
Kidney Int. 51 (4): 1059-68.
Cisternas, P.A. & Armati, P.J. (2000) Immune system cell markers in the northern brown bandicoot, Isoodon macrourus.
Dev Comp Immunol. 24 (8): 771-82.
Pabst, R. et al. (2009) TLR2/6 stimulation of the rat lung: effects on lymphocyte subsets, natural killer cells and dendritic cells in different parts of the air-conducting compartments and at different ages.
Immunology. 126 (1): 132-9.
Pérez-cano, F.J. et al. (2007) Phenotypic and functional characteristics of rat spleen lymphocytes during suckling.
Dev Comp Immunol. 31 (12): 1264-77.
Mccormack, E. et al. (2010) Lentinan: hematopoietic, immunological, and efficacy studies in a syngeneic model of acute myeloid leukemia.
Nutr Cancer. 62 (5): 574-83.
Emara, A.M. & Draz, E.I. (2007) Immunotoxicological study of one of the most common over-the-counter pyrethroid insecticide products in Egypt.
Inhal Toxicol. 19 (12): 997-1009.
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