Kappa Light Chain antibody | OX-12

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Mouse anti Rat Kappa Light Chain:HRP

Product Type
Monoclonal Antibody
Clone
OX-12
Isotype
IgG2a
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
MCA49P E 0.5 mg
Mouse anti Rat Kappa Light Chain antibody, clone OX-12 recognizes rat kappa chains, showing a marked preference for binding to the Igka allotype which is present in AGUS, AO, AUG, BN, LEW, LOU, PVG and WAG strains but not in ACI, DA, F344, MSU or SHR rat strains.

This product is routinely tested in flow cytometry on rat splenic lymphocytes.

Product Details

Target Species
Rat
Product Form
Purified IgG conjugated to Horseradish Peroxidase (HRP) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.01%Thiomersal
Immunogen
F(ab')2 fragment of rat IgG
Approx. Protein Concentrations
IgG concentration 1.0 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice fused with cells of the mouse NS1 myeloma cell line.

Storage Information

Storage
Store at +4oC or at -20oC if preferred.

This product should be stored undiluted.

Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.

More Information

UniProt
P01836 Related reagents
P01835 Related reagents
Entrez Gene
LOC500180 Related reagents
GO Terms
GO:0003823 antigen binding
Regulatory
For research purposes only

Applications of Kappa Light Chain antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/500 1/1000
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Pack Size Applications List Price Quantity
AbGUARD® HRP Stabilizer Plus BUF052A 100 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052B 500 ml C E P WB
AbGUARD® HRP Stabilizer Plus BUF052C 1000 ml C E P WB
TMB Signal+ BUF054A 100 ml E
TMB Core BUF056A 100 ml E
TMB Core+ BUF062A 100 ml E

Product Specific References

References for Kappa Light Chain antibody

  1. Hunt, S.V. & Fowler, M.H. (1981) A repopulation assay for B and T lymphocyte stem cells employing radiation chimaeras.
    Cell Tissue Kinet. 14 (4): 445-64.
  2. Bukovsk√Ĺ, A. et al. (1984) Association of some cell surface antigens of lymphoid cells and cell surface differentiation antigens with early rat pregnancy.
    Immunology. 52 (4): 631-40.
  3. Balogh, P. et al. (2002) Simultaneous blockade of Fc gamma receptors and indirect labeling of mouse lymphocytes by the selective detection of allotype-restricted epitopes on the kappa chain of rat monoclonal antibodies.
    Cytometry. 47 (2): 107-10.
  4. Lozano, F. et al. (1993) Affinity maturation leads to differential expression of multiple copies of a kappa light-chain transgene.
    Nature. 363 (6426): 271-3.
  5. Gehrs, B.C. et al. (1997) Alterations in the developing immune system of the F344 rat after perinatal exposure to 2,3,7,8-tetrachlorodibenzo-p-dioxin: II. Effects on the pup and the adult.
    Toxicology. 122 (3): 229-40.
  6. Penny, M.J. et al. (1997) Role of T cells in the mediation of Heymann nephritis. ii. Identification of Th1 and cytotoxic cells in glomeruli.
    Kidney Int. 51 (4): 1059-68.
  7. Cisternas, P.A. & Armati, P.J. (2000) Immune system cell markers in the northern brown bandicoot, Isoodon macrourus.
    Dev Comp Immunol. 24 (8): 771-82.
  8. Pabst, R. et al. (2009) TLR2/6 stimulation of the rat lung: effects on lymphocyte subsets, natural killer cells and dendritic cells in different parts of the air-conducting compartments and at different ages.
    Immunology. 126 (1): 132-9.
  9. Pérez-cano, F.J. et al. (2007) Phenotypic and functional characteristics of rat spleen lymphocytes during suckling.
    Dev Comp Immunol. 31 (12): 1264-77.
  10. Mccormack, E. et al. (2010) Lentinan: hematopoietic, immunological, and efficacy studies in a syngeneic model of acute myeloid leukemia.
    Nutr Cancer. 62 (5): 574-83.
  11. Emara, A.M. & Draz, E.I. (2007) Immunotoxicological study of one of the most common over-the-counter pyrethroid insecticide products in Egypt.
    Inhal Toxicol. 19 (12): 997-1009.