CD63 antibody | AD1

Mouse anti Rat CD63:Alexa Fluor®647

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
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Search for Batch Specific Datasheets

Mouse anti Rat CD63 antibody, clone AD1 recognizes rat CD63, an intracellular type-III lysosomal glycoprotein and member of the tetraspanin (TM4SF) family, found on activated platelets and resident macrophages. Rat CD63 is expressed on mast cells in tissues and can be induced on other cell types in vitro (Nishikata et al. 1992).

Mouse anti Rat CD63 antibody, clone AD1 partially inhibits IgE-mediated histamine release and binding competition assays indicate that it is sterically situated close to the high-affinity IgE receptor on rat basophilic leukemia (RBL-2H3) cells (Kitani et al. 1991).

Target Species
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
RBL-2H3 rat basophilic leukaemia cell line.
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63-Ag8-653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
For research purposes only
12 months from date of despatch
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 106 cells in 100ul.

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:Alexa Fluor® 647 MCA1209A647 F 100 Tests/1ml loader
List Price Your Price
Description Mouse IgG1 Negative Control:Alexa Fluor® 647

Source Reference

  1. Kitani, S. et al. (1991) A cell surface glycoprotein of rat basophilic leukemia cells close to the high affinity IgE receptor (Fc epsilon RI). Similarity to human melanoma differentiation antigen ME491.
    J Biol Chem. 266 (3): 1903-9.

Antibody Characterization Reference

  1. Nishikata, H. et al. (1992) The rat mast cell antigen AD1 (homologue to human CD63 or melanoma antigen ME491) is expressed in other cells in culture.
    J Immunol. 149(3): 862-70. J Immunol. 149(3): 862-70.

References for CD63 antibody

  1. Kraft, S. et al. (2005) Anti-CD63 antibodies suppress IgE-dependent allergic reactions in vitro and in vivo.
    J Exp Med. 201: 385-96.
  2. Boros, M. et al. (1995) Response of mucosal mast cells to intestinal ischemia-reperfusion injury in the rat.
    Shock. 3 (2): 125-31.
  3. Vincent-Schneider, H. et al. (2001) Secretory granules of mast cells accumulate mature and immature MHC class II molecules
    J Cell Sci. 114(Pt 2): 323-34.
  4. Ramezani-Moghadam, M. et al. (2015) Adiponectin Reduces Hepatic Stellate Cell Migration by Promoting Tissue Inhibitor of Metalloproteinase-1 (TIMP-1) Secretion.
    J Biol Chem. 290(9): 5533-42.
  5. Yoshimura, A. et al. (2016) Generation of a novel transgenic rat model for tracing extracellular vesicles in body fluids.
    Sci Rep. 6: 31172.
  6. Pasquier, A. et al. (2019) Lysosomal degradation of newly formed insulin granules contributes to β cell failure in diabetes.
    Nat Commun. 10 (1): 3312.


Western Blotting

Entrez Gene
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0005765 lysosomal membrane
GO:0030658 transport vesicle membrane
GO:0010633 negative regulation of epithelial cell migration
GO:0030855 epithelial cell differentiation
GO:0032403 protein complex binding
GO:0043234 protein complex
GO:0045785 positive regulation of cell adhesion
GO:0045807 positive regulation of endocytosis
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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