CD61 antibody | F11
Mouse anti Rat CD61:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- F11
- Isotype
- IgG1
- Specificity
- CD61
Antibody Quality and Performance Guarantee
| Mouse anti Rat CD61 antibody, clone F11 recognizes the rat CD61 cell surface antigen, also known as the integrin b3 chain. CD61 is expressed by platelets, megakaryocytes and osteoclasts.Clone F11 has been shown to inhibit the in vitro attachment of osteoclasts to various proteins including vitronectin, fibrinogen and von Willebrand Factor (Sick et al. 2009). |
- Target Species
- Rat
- Species Cross-Reactivity
-
Target Species Cross Reactivity Human Weak - N.B. Antibody reactivity and working conditions may vary between species.
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Bone cell suspension from SD rats.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized Balb/c mice were fused with the cells of the mouse X63-Ag8 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA1209A647 | F | 100 Tests/1ml |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 | ||||||
References for CD61 antibody
-
Helfrich, M.H. et al. (1992) Integrins on rat osteoclasts: characterization of two monoclonal antibodies (F4 and F11) to rat beta 3.
J Bone Miner Res. 7 (3): 345-51. -
Shankar, G. et al. (1993) Integrin receptor-mediated mobilisation of intranuclear calcium in rat osteoclasts.
J Cell Sci. 105 ( Pt 1): 61-8. -
Sick, E. et al. (2009) Activation of CD47 receptors causes histamine secretion from mast cells.
Cell Mol Life Sci. 66: 1271-82. -
Wang, Z. et al. (2016) Microparticle levels after arterial injury and NO therapy in diabetes.
J Surg Res. 200 (2): 722-31.
- Synonyms
- Integrin Beta 3 Chain
- RRID
- AB_566857
MCA1773A647
1608
If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
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