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CD2 antibody | OX-34

Mouse anti Rat CD2:Alexa Fluor® 647

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/1ml loader
List Price Your Price

Mouse anti Rat CD2 antibody, clone OX-34 recognizes a determinant on thymocytes and peripheral T-cells but it does not bind to B cells or peritoneal macrophages. The antigen recognized by this antibody is a 50-54 kDa glycoprotein, homolog of the human CD2 antigen (Williams et al. 1987).

Target Species
Product Form
Purified IgG conjugated to Alexa Fluor® 647 - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Activated rat T helper cells.
Approx. Protein Concentrations
IgG concentration 0.05 mg/ml
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
Alexa Fluor®647 650 665
For research purposes only
12 months from date of despatch
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG2a Negative Control:Alexa Fluor® 647 MCA1210A647 F 100 Tests/1ml loader
List Price Your Price
Description Mouse IgG2a Negative Control:Alexa Fluor® 647

References for CD2 antibody

  1. Williams, A.F. et al. (1987) Similarities in sequences and cellular expression between rat CD2 and CD4 antigens.
    J Exp Med. 165 (2): 368-80.
  2. Barclay, A.N. (1981) The localization of populations of lymphocytes defined by monoclonal antibodies in rat lymphoid tissues.
    Immunology. 42 (4): 593-600.
  3. Whiteland, J.L. et al. (1995) Immunohistochemical detection of T-cell subsets and other leukocytes in paraffin-embedded rat and mouse tissues with monoclonal antibodies.
    J Histochem Cytochem. 43 (3): 313-20.
  4. Baker, S.C. et al. (2011) Cellular Integration and Vascularisation Promoted by a Resorbable, Particulate-Leached, Cross-Linked Poly(ε-caprolactone) Scaffold.
    Macromol Biosci. 11: 618-27.
  5. Romani, P. et al. (2009) Cell survival and polarity of Drosophila follicle cells require the activity of ecdysone receptor B1 isoform.
    Genetics. 181: 165-75.
  6. Bastock, R. et al. (2003) Strabismus is asymmetrically localised and binds to Prickle and Dishevelled during Drosophila planar polarity patterning.
    Development. 130: 3007-14.
  7. Brückner, K. et al. (2000) Glycosyltransferase activity of Fringe modulates Notch-Delta interactions.
    Nature. 406: 411-5.
  8. Liversidge, J. et al. (2002) Nitric oxide mediates apoptosis through formation of peroxynitrite and Fas/Fas-ligand interactions in experimental autoimmune uveitis.
    Am J Pathol. 160: 905-16.
  9. View The Latest Product References
  10. Sarpal, R. et al. (2012) Mutational analysis supports a core role for Drosophila α-catenin in adherens junction function.
    J Cell Sci. 125: 233-45.
  11. Zhang, H. et al. (2011) Basic residues in the T-cell receptor ζ cytoplasmic domain mediate membrane association and modulate signaling.
    Proc Natl Acad Sci U S A. 108: 19323-8.
  12. Heck, B.W. et al. (2012) The transcriptional corepressor SMRTER influences both Notch and ecdysone signaling during Drosophila development.
    Biol Open. 1 (3): 182-96.
  13. Clark, I.B. et al. (2011) Fibroblast growth factor signalling controls successive cell behaviours during mesoderm layer formation in Drosophila.
    Development. 138: 2705-15.
  14. Domanitskaya, E. and Schüpbach, T. (2012) CoREST acts as a positive regulator of Notch signaling in the follicle cells of Drosophila melanogaster.
    J Cell Sci. 125: 399-410.
  15. Dragovic, R.A. et al. (2015) Isolation of syncytiotrophoblast microvesicles and exosomes and their characterisation by multicolour flow cytometry and fluorescence Nanoparticle Tracking Analysis.
    Methods. 87: 64-74.
  16. Zecca, M. & Struhl, G. (2021) A unified mechanism for the control of Drosophila. wing growth by the morphogens Decapentaplegic and Wingless.
    PLoS Biol. 19 (3): e3001111.



E-Rosette Receptor
Entrez Gene
GO Terms
GO:0001948 glycoprotein binding
GO:0003823 antigen binding
GO:0016021 integral to membrane
GO:0005615 extracellular space
GO:0030971 receptor tyrosine kinase binding
GO:0009986 cell surface
GO:0016337 cell-cell adhesion
GO:0019901 protein kinase binding
GO:0042110 T cell activation
GO:0042803 protein homodimerization activity
GO:0043234 protein complex
GO:0043621 protein self-association
GO:0044214 fully spanning the plasma membrane
GO:0045121 membrane raft



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