CD161 antibody | 10/78
Mouse anti Rat CD161:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- 10/78
- Isotype
- IgG1
- Specificity
- CD161
Mouse anti Rat CD161 antibody, clone 10/78 recognizes the rat Killer cell lectin-like receptor subfamily B protein, also known as NKR-PI or CD161. CD161 is a 233 amino acid ~60 kDa type II single pass protein containing a single C-type lectin domain. CD161 is expressed on rat NK cells and T cell subpopulations. CD161 exists in 2 forms NKR-PIa and NKR-PIb, Mouse anti Rat CD161 antibody, clone 10/78 recognizes both forms of CD161 (Li et al. 2003). Clone 10/78 competes with another anti CD161 clone, 3.2.3 for binding to antigen. Mouse anti Rat CD161 antibody, clone 10/78 has been successfully employed for the in vivo depletion of rat NK cells in an experimental obesity model (Wrann et al. 2010). |
- Target Species
- Rat
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Purified splenic NK cells from the LEW rat strain.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63.Ag8653 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | Neat | 1/10 |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
---|---|---|---|---|---|---|---|
Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA1209A647 | F | 100 Tests/1ml | Log in | |||
List Price | Your Price | ||||||
Log in | |||||||
Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 |
References for CD161 antibody
-
Sedgwick, J.D. et al. (1998) Central nervous system microglial cell activation and proliferation follows direct interaction with tissue-infiltrating T cell blasts.
J Immunol. 160 (11): 5320-30. -
Ikezumi, Y. et al. (2000) An anti-CD5 monoclonal antibody ameliorates proteinuria and glomerular lesions in rat mesangioproliferative glomerulonephritis.
Kidney Int. 58 (1): 100-14. -
Tliba, O. et al. (2002) Evaluation of the hepatic NK cell response during the early phase of Fasciola hepatica infection in rats.
Vet Res. 33 (3): 327-32. -
Banerjee, S. et al. (2003) Development of organised conjunctival leucocyte aggregates after corneal transplantation in rats.
Br J Ophthalmol. 87: 1515-22. -
Dyugovskaya, L. et al. (2003) Phenotypic profile and functional characterization of rat lymph node-derived gammadelta T cells: implication in the immune response to cytomegalovirus.
Immunology. 108 (2): 129-36. -
Ali, S. et al. (2005) Combined immunostimulation and conditional cytotoxic gene therapy provide long-term survival in a large glioma model.
Cancer Res. 65: 7194-204. -
Obara, H. et al. (2005) IFN-gamma, produced by NK cells that infiltrate liver allografts early after transplantation, links the innate and adaptive immune responses.
Am J Transplant. 5 (9): 2094-103. -
Latta, M. et al. (2007) CXCR6 is expressed on T cells in both T helper type 1 (Th1) inflammation and allergen-induced Th2 lung inflammation but is only a weak mediator of chemotaxis.
Immunology. 121: 555-64.
View The Latest Product References
-
Blöcher, S. et al. (2007) Acute rejection of experimental lung allografts: characterization of intravascular mononuclear leukocytes.
Clin Immunol. 124 (1): 98-108. -
Schwartzkopff, J. et al. (2010) NK cell depletion delays corneal allograft rejection in baby rats.
Mol Vis. 16: 1928-35. -
Wrann, C.D. et al. (2010) Obesity and NK cells affect the expression of the long form of the leptin receptor Ob-Rb in liver of F344 rats.
Exp Toxicol Pathol. 62 (1): 1-8. -
Lyons, A. et al. (2011) Atorvastatin prevents age-related and amyloid-beta-induced microglial activation by blocking interferon-gamma release from natural killer cells in the brain.
J Neuroinflammation. 8: 27. -
Kuper, C.F. et al. (2011) Oxazolone (OXA) is a respiratory allergen in Brown Norway rats.
Toxicology. 290 (1): 59-68. -
Lee, J.S. et al. (2011) Immunomodulatory effect of mushrooms on cytotoxic activity and cytokine production of intestinal lamina propria leukocytes does not necessarily depend on β-glucan contents.
Food Chem. 126 (4): 1521-6. -
Trama, A.M. et al. (2012) Lymphocyte phenotypes in wild-caught rats suggest potential mechanisms underlying increased immune sensitivity in post-industrial environments.
Cell Mol Immunol. 9 (2): 163-74. -
Beutel, G. et al. (2013) Effect of chronic elevated asymmetric dimethylarginine (ADMA) levels on granulopoiesis.
Ann Hematol. 92 (4): 505-8. -
Arsenović-Ranin, N. et al. (2013) Ovarian hormone withdrawal in prepubertal developmental stage does not prevent thymic involution in rats.
Exp Biol Med (Maywood). 238 (6): 641-57. -
Arndt, T. et al. (2014) Variable immune cell frequencies in peripheral blood of LEW.1AR1-iddm rats over time compared to other congenic LEW strains.
Clin Exp Immunol. 177 (1): 168-78. -
Djikić J et al. (2014) Age-associated changes in rat immune system: lessons learned from experimental autoimmune encephalomyelitis.
Exp Gerontol. 58: 179-97. -
Koch, M. et al. (2015) Extracellular Vesicles from MSC Modulate the Immune Response to Renal Allografts in a MHC Disparate Rat Model.
Stem Cells Int. 2015: 486141. -
Lemke, A. et al. (2015) Rat renal transplant model for mixed acute humoral and cellular rejection: Weak correlation of serum cytokines/chemokines with intragraft changes.
Transpl Immunol. 33 (2): 95-102. -
Williamson, L.L. et al. (2016) Got worms? Perinatal exposure to helminths prevents persistent immune sensitization and cognitive dysfunction induced by early-life infection.
Brain Behav Immun. 51: 14-28. -
Bähr, I. et al. (2017) Diet-Induced Obesity Is Associated with an Impaired NK Cell Function and an Increased Colon Cancer Incidence.
J Nutr Metab. 2017: 4297025. -
Sun, C.K. et al. (2017) Melatonin treatment enhances therapeutic effects of exosomes against acute liver ischemia-reperfusion injury.
Am J Transl Res. 9 (4): 1543-60. -
Chang, J.C. et al. (2019) Early Immune Response to Acute Gastric Fluid Aspiration in a Rat Model of Lung Transplantation.
Exp Clin Transplant. 17 (1): 84-92. -
Xing, Z. et al. (2021) Early Toll-like receptor 4 inhibition improves immune dysfunction in the hippocampus after hypoxic-ischemic brain damage
Int J Med Sci. 19 (1): 142-51. -
Zhang, P. et al. (2019) The Effect of Gamma-Ray-Induced Central Nervous System Injury on Peripheral Immune Response: An In Vitro and In Vivo Study.
Radiat Res. 192 (4): 440-450.
- Synonyms
- NKR-P1A
- RRID
- AB_322589
- UniProt
- P27471
- A4KWA1
- Entrez Gene
- Klrb1b
- Klrb1a
- GO Terms
- GO:0016021 integral to membrane
- GO:0004872 receptor activity
- GO:0005529 sugar binding
MCA1427A647
152138If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
View all Anti-Rat ProductsAlways be the first to know.
When we launch new products and resources to help you achieve more in the lab.
Yes, sign me up