Leishmania LPG antibody | CA7AE

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Mouse anti Leishmania LPG (Repeat Epitope)

Product Type
Monoclonal Antibody
Clone
CA7AE
Isotype
IgM
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
OBT2002 E IB IF 0.5 ml
Mouse anti Leishmania lipophosphoglycan antibody, clone CA7AE recognizes lipophosphoglycan (LPG) the major cell surface glycoconjugate of Leishmania parasites. Mouse anti Leishmania lipophosphoglycan antibody, clone CA7AE recognizes the repeat carbohydrate epitope of most species of Leishmania LPG. The epitope is also found on the excreted acid phosphatase of Leishmania and is expressed on the surface of Leishmania infected macrophages (Tolson et al. 1990).

Mouse anti Leishmania lipophosphoglycan antibody, clone CA7AE recognizes the promastigotes of Leishmania donovani but not those of the related species L. tropica (Jaffe and Sarfstein 1987, Sundar et al. 2001). Mouse anti Leishmania lipophosphoglycan antibody, clone CA7AE does however recognize a broad range of L. donovani and L. major strains and related species including L. infantum, L. m. mexicana, L. aethiopica and L. b. panamensis (Tolson et al. 1994).

Product Details

Target Species
Protozoan
Product Form
Ascites - lyophilised
Reconstitution
Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended.
Preservative Stabilisers
None present
Immunogen
Heat killed Leishmania donovani promastigotes.
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the murine SP2/0 myeloma cell line.

Storage Information

Storage
Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody.
Shelf Life
12 months from date of reconstitution.

More Information

Regulatory
For research purposes only

Applications of Leishmania LPG antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/1000
Immunoblotting
Immunofluorescence 1/500 1/1000
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Application Based External Images

Functional Assays

Immunofluorescence

Western Blotting

Product Specific References

References for Leishmania LPG antibody

  1. Sundar, S. et al. (2001) Resistance to treatment in Kala-azar: speciation of isolates from northeast India.
    Am J Trop Med Hyg. 65: 193-6.
  2. Tolson, D.L. et al. (1990) Expression of a repeating phosphorylated disaccharide lipophosphoglycan epitope on the surface of macrophages infected with Leishmania donovani.
    Infect Immun. 58: 3500-7.
  3. Butcher, B.A. et al. (1996) Deficiency in beta1,3-galactosyltransferase of a Leishmania major lipophosphoglycan mutant adversely influences the Leishmania-sand fly interaction.
    J Biol Chem. 271: 20573-9.
  4. Goyard, S. et al. (2003) An in vitro system for developmental and genetic studies of Leishmania donovani phosphoglycans
    Mol Biochem Parasitol. 130: 31-42.
  5. Soares, R.P. et al. (2004) Leishmania tropica: intraspecific polymorphisms in lipophosphoglycan correlate with transmission by different Phlebotomus species.
    Exp Parasitol. 107: 105-14.
  6. Amprey, J.L. et al. (2004) Inhibition of CD1 expression in human dendritic cells during intracellular infection with Leishmania donovani.
    Infect Immun. 72: 589-92.
  7. Coelho-Finamore, J.M. et al. (2011) Leishmania infantum: Lipophosphoglycan intraspecific variation and interaction with vertebrate and invertebrate hosts.
    Int J Parasitol. 41: 333-42.
  8. Capul, A.A. et al. (2007) Two Functionally Divergent UDP-Gal Nucleotide Sugar Transporters Participate in Phosphoglycan Synthesis in Leishmania major
    J Biol Chem. 282: 14006-17.
  9. Vinet, A.F. et al. (2009) The Leishmania donovani lipophosphoglycan excludes the vesicular proton-ATPase from phagosomes by impairing the recruitment of synaptotagmin V.
    PLoS Pathog. 5: e1000628.