Leishmania LPG antibody | CA7AE






Mouse anti Leishmania lipophosphoglycan antibody, clone CA7AE recognizes the promastigotes of Leishmania donovani but not those of the related species L. tropica (Jaffe and Sarfstein 1987, Sundar et al. 2001). Mouse anti Leishmania lipophosphoglycan antibody, clone CA7AE does however recognize a broad range of L. donovani and L. major strains and related species including L. infantum, L. m. mexicana, L. aethiopica and L. b. panamensis (Tolson et al. 1994).
Product Details
- Target Species
- Protozoan
- Product Form
- Ascites - lyophilised
- Reconstitution
- Reconstitute with 0.5 ml distilled water
Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. For long term storage the addition of 0.09% sodium azide is recommended. - Preservative Stabilisers
- None present
- Immunogen
- Heat killed Leishmania donovani promastigotes.
- Fusion Partners
- Spleen cells from immunised BALB/c mice were fused with cells of the murine SP2/0 myeloma cell line.
Storage Information
- Storage
- Prior to reconstitution store at +4oC.
After reconstitution store at -20oC.
Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. - Guarantee
- 12 months from date of despatch
More Information
- Regulatory
- For research purposes only
Applications of Leishmania LPG antibody
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
ELISA | 1/1000 | ||
Immunoblotting | |||
Immunofluorescence | 1/500 | 1/1000 |
Product Specific References
References for Leishmania LPG antibody
-
Sundar, S. et al. (2001) Resistance to treatment in Kala-azar: speciation of isolates from northeast India.
Am J Trop Med Hyg. 65: 193-6. -
Tolson, D.L. et al. (1990) Expression of a repeating phosphorylated disaccharide lipophosphoglycan epitope on the surface of macrophages infected with Leishmania donovani.
Infect Immun. 58: 3500-7. -
Butcher, B.A. et al. (1996) Deficiency in beta1,3-galactosyltransferase of a Leishmania major lipophosphoglycan mutant adversely influences the Leishmania-sand fly interaction.
J Biol Chem. 271: 20573-9. -
Goyard, S. et al. (2003) An in vitro system for developmental and genetic studies of Leishmania donovani phosphoglycans
Mol Biochem Parasitol. 130: 31-42. -
Soares, R.P. et al. (2004) Leishmania tropica: intraspecific polymorphisms in lipophosphoglycan correlate with transmission by different Phlebotomus species.
Exp Parasitol. 107: 105-14. -
Amprey, J.L. et al. (2004) Inhibition of CD1 expression in human dendritic cells during intracellular infection with Leishmania donovani.
Infect Immun. 72: 589-92. -
Coelho-Finamore, J.M. et al. (2011) Leishmania infantum: Lipophosphoglycan intraspecific variation and interaction with vertebrate and invertebrate hosts.
Int J Parasitol. 41: 333-42. -
Capul, A.A. et al. (2007) Two Functionally Divergent UDP-Gal Nucleotide Sugar Transporters Participate in Phosphoglycan Synthesis in Leishmania major
J Biol Chem. 282: 14006-17. -
Vinet, A.F. et al. (2009) The Leishmania donovani lipophosphoglycan excludes the vesicular proton-ATPase from phagosomes by impairing the recruitment of synaptotagmin V.
PLoS Pathog. 5: e1000628.
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