Histidine Rich Protein II antibody | AbD14971_hIgG1
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FLA ResetHuman anti Plasmodium falciparum HRPII
- Product Type
- Monoclonal Antibody
- Clone
- AbD14971_hIgG1
- Isotype
- IgG1
- Specificity
- Histidine Rich Protein II
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| Human anti Plasmodium falciparum HRPII, clone AbD14971 recognizes HRPII (histidine rich protein II) from the Malaria causing protozoan species Plasmodium falciparum. Clone AbD14971 was selected by phage display under heat stress conditions. The Tm value for thermal denaturation of the antibody was measured by CD spectroscopy and has been found to be 70 °C. Human anti Plasmodium falciparum HRPII, clone AbD14971 is intended for use as a capture antibody with Human anti Plasmodium falciparum HRPII antibody, clone AbD14964 (HCA159P) as the detection antibody in sandwich assay development. |
- Target Species
- Protozoan
- Product Form
- Human IgG1 antibody (lambda light chain) selected from the HuCAL ® phage display library and expressed in a human cell line. This product is supplied as liquid.
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Immunogen
- Recombinant HRPII fusion protein from Plasmodium falciparum.
- Approx. Protein Concentrations
- IgG concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Technical Advice
- Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
- ELISA
- This product is suitable for use in indirect ELISA applications. This product may be used as a capture reagent in a sandwich ELISA together with HCA159P as the detection reagent.
- RRID
- AB_10896836
- UniProt
- Q25878
- Licensed Use
- For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
View more products with HISTIDINE specificity
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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