Cryptosporidium antibody | BEL 0126
Mouse anti Cryptosporidium:FITC
- Product Type
- Monoclonal Antibody
- Clone
- BEL 0126
- Isotype
- IgG3
- Specificity
- Cryptosporidium
Mouse anti Cryptosporidium antibody, clone BEL 0126 recognizes a membrane antigen expressed by the oocysts of Cryptosporidium sp., an obligate enteric coccidian parasite of the phylum Apicomplexa that infects the gastrointestinal tract. The parasite is one of the most important enteric pathogens in both humans and animals (Rose et al. 2002). Since its first diagnosis in 1975 (Meisel et al. 1976), Cryptosporidiosis, has become one of the most prominent public health concerns worldwide (Rose et al. 2002). Cryptosporidium oocysts are resistant to chlorine and their small size makes removal by filtration difficult. Alternative methods have been developed such as UV and ozone treatment alongside monitoring using Immunofluorescence screening (Rose et al. 2002). Cryptosporidiosis is a disease affecting the intestines of mammals which is spread through the fecal-oral route. The main symptom of is self-limiting diarrhea in people with intact immune systems. However, in immunocompromised individuals, such as AIDS patients, infection can cause permanent & life-threatening diarrhea (Ma et al. 1984). |
- Target Species
- Protozoan
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide (NaN3) 1% Bovine serum albumin - Immunogen
- Purified Cryptosporidium oocysts from bovine faeces.
- Approx. Protein Concentrations
- IgG concentration 0.1mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/5 | 1/50 | |
Immunofluorescence |
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
References for Cryptosporidium antibody
-
Mansfield, K.G. et al. (1997) Identification of an Enterocytozoon bieneusi-like microsporidian parasite in simian-immunodeficiency-virus-inoculated macaques with hepatobiliary disease.
Am J Pathol. 150: 1395-405. -
Weigum, S.E. et al. (2016) Hollow silica microspheres for buoyancy-assisted separation of infectious pathogens from stool.
J Chromatogr A. 1466: 29-36. -
Luka, G. et al. (2019) Label-Free Capacitive Biosensor for Detection of Cryptosporidium.
Sensors (Basel). 19 (2)Jan 10 [Epub ahead of print]. -
Sonzogni-desautels, K. et al. (2019) A protocol to count Cryptosporidium oocysts by flow cytometry without antibody staining.
PLoS Negl Trop Dis. 13 (3): e0007259. -
Luka, G.S. et al. (2022) On-chip-based electrochemical biosensor for the sensitive and label-free detection of Cryptosporidium..
Sci Rep. 12 (1): 6957.
Further Reading
-
Meisel, J.L. et al. (1976) Overwhelming watery diarrhea associated with a cryptosporidium in an immunosuppressed patient.
Gastroenterology. 70: 1156-60. -
Rose, J.B. et al. (2002) Risk and control of waterborne cryptosporidiosis.
FEMS Microbiol Rev. 26: 113-23. -
Ma, P. (1984) Cryptosporidium and the enteropathy of immune deficiency.
J Pediatr Gastroenterol Nutr. 3 (4): 488-90.
- RRID
- AB_808536
2402-3007F
1606If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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