Cryptosporidium antibody | BEL 0126
Mouse anti Cryptosporidium:Alexa Fluor® 488
- Product Type
- Monoclonal Antibody
- Clone
- BEL 0126
- Isotype
- IgG3
- Specificity
- Cryptosporidium
Mouse anti Cryptosporidium antibody, clone BEL 0126 recognizes a membrane antigen expressed by the oocysts of Cryptosporidium sp., an obligate enteric coccidian parasite of the phylum Apicomplexa that infects the gastrointestinal tract. The parasite is one of the most important enteric pathogens in both humans and animals (Rose et al. 2002). Since its first diagnosis in 1975 (Meisel et al. 1976), Cryptosporidiosis, has become one of the most prominent public health concerns worldwide (Rose et al. 2002). Cryptosporidium oocysts are resistant to chlorine and their small size makes removal by filtration difficult. Alternative methods have been developed such as UV and ozone treatment alongside monitoring using Immunofluorescence screening (Rose et al. 2002). Cryptosporidiosis is a disease affecting the intestines of mammals which is spread through the fecal-oral route. The main symptom of is self-limiting diarrhea in people with intact immune systems. However, in immunocompromised individuals, such as AIDS patients, infection can cause permanent & life-threatening diarrhea (Ma et al. 1984). |
- Target Species
- Protozoan
- Product Form
- Purified IgG conjugated to Alexa Fluor 488 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Purified Cryptosporidium oocysts from bovine faeces.
- Approx. Protein Concentrations
- IgG concentration 0.05mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®488 495 519 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
Application Name | Verified | Min Dilution | Max Dilution |
---|---|---|---|
Flow Cytometry | 1/5 | 1/20 |
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 1x106 cells in 100μl
References for Cryptosporidium antibody
-
Mansfield, K.G. et al. (1997) Identification of an Enterocytozoon bieneusi-like microsporidian parasite in simian-immunodeficiency-virus-inoculated macaques with hepatobiliary disease.
Am J Pathol. 150: 1395-405. -
Weigum, S.E. et al. (2016) Hollow silica microspheres for buoyancy-assisted separation of infectious pathogens from stool.
J Chromatogr A. 1466: 29-36. -
Luka, G. et al. (2019) Label-Free Capacitive Biosensor for Detection of Cryptosporidium.
Sensors (Basel). 19 (2)Jan 10 [Epub ahead of print]. -
Sonzogni-desautels, K. et al. (2019) A protocol to count Cryptosporidium oocysts by flow cytometry without antibody staining.
PLoS Negl Trop Dis. 13 (3): e0007259. -
Luka, G.S. et al. (2022) On-chip-based electrochemical biosensor for the sensitive and label-free detection of Cryptosporidium..
Sci Rep. 12 (1): 6957.
Further Reading
-
Meisel, J.L. et al. (1976) Overwhelming watery diarrhea associated with a cryptosporidium in an immunosuppressed patient.
Gastroenterology. 70: 1156-60. -
Rose, J.B. et al. (2002) Risk and control of waterborne cryptosporidiosis.
FEMS Microbiol Rev. 26: 113-23. -
Ma, P. (1984) Cryptosporidium and the enteropathy of immune deficiency.
J Pediatr Gastroenterol Nutr. 3 (4): 488-90.
- RRID
- AB_844533
2402-3007A488
151140If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.
Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"
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