Cryptosporidium antibody | BEL 0126

Mouse anti Cryptosporidium

Product Type
Monoclonal Antibody
Clone
BEL 0126
Isotype
IgG3
Specificity
Cryptosporidium

Product Code Applications Pack Size List Price Your Price Qty
2402-3007G
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F IF P 1 mg loader
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loader
2402-3007
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E F IF P 0.2 mg loader
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Mouse anti Cryptosporidium antibody, clone BEL 0126 recognizes a membrane antigen expressed by the oocysts of Cryptosporidium sp., an obligate enteric coccidian parasite of the phylum Apicomplexa that infects the gastrointestinal tract. The parasite is one of the most important enteric pathogens in both humans and animals (Rose et al. 2002). Since its first diagnosis in 1975 (Meisel et al. 1976), Cryptosporidiosis, has become one of the most prominent public health concerns worldwide (Rose et al. 2002).

Cryptosporidium oocysts are resistant to chlorine and their small size makes removal by filtration difficult. Alternative methods have been developed such as UV and ozone treatment alongside monitoring using Immunofluorescence screening (Rose et al. 2002).

Cryptosporidiosis is a disease affecting the intestines of mammals which is spread through the fecal-oral route. The main symptom of is self-limiting diarrhea in people with intact immune systems. However, in immunocompromised individuals, such as AIDS patients, infection can cause permanent & life-threatening diarrhea (Ma et al. 1984).

Target Species
Protozoan
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Purified cryptosporidium oocysts from bovine faeces.
Approx. Protein Concentrations
1.0 mg/ml
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1/2000 1/8000
Flow Cytometry
Immunofluorescence 1/50 1/200
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.

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References for Cryptosporidium antibody

  1. Mansfield, K.G. et al. (1997) Identification of an Enterocytozoon bieneusi-like microsporidian parasite in simian-immunodeficiency-virus-inoculated macaques with hepatobiliary disease.
    Am J Pathol. 150: 1395-405.
  2. Weigum, S.E. et al. (2016) Hollow silica microspheres for buoyancy-assisted separation of infectious pathogens from stool.
    J Chromatogr A. 1466: 29-36.
  3. Luka, G. et al. (2019) Label-Free Capacitive Biosensor for Detection of Cryptosporidium.
    Sensors (Basel). 19 (2)Jan 10 [Epub ahead of print].
  4. Sonzogni-desautels, K. et al. (2019) A protocol to count Cryptosporidium oocysts by flow cytometry without antibody staining.
    PLoS Negl Trop Dis. 13 (3): e0007259.
  5. Luka, G.S. et al. (2022) On-chip-based electrochemical biosensor for the sensitive and label-free detection of Cryptosporidium..
    Sci Rep. 12 (1): 6957.

Further Reading

  1. Meisel, J.L. et al. (1976) Overwhelming watery diarrhea associated with a cryptosporidium in an immunosuppressed patient.
    Gastroenterology. 70: 1156-60.
  2. Rose, J.B. et al. (2002) Risk and control of waterborne cryptosporidiosis.
    FEMS Microbiol Rev. 26: 113-23.
  3. Ma, P. (1984) Cryptosporidium and the enteropathy of immune deficiency.
    J Pediatr Gastroenterol Nutr. 3 (4): 488-90.

RRID
AB_616904
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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