Cryptosporidium antibody | BEL 0126

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Mouse anti Cryptosporidium:Alexa Fluor® 488

Mouse anti Cryptosporidium:FITC

Mouse anti Cryptosporidium

Product Type
Monoclonal Antibody
Clone
BEL 0126
Isotype
IgG3
Product CodeApplicationsDatasheetMSDSPack SizeList PriceQuantity
2402-3007A488 F 100 Tests
2402-3007F F IF 0.1 mg
2402-3007 E F IF P 0.2 mg
2402-3007G E F IF P 1 mg
Mouse anti Cryptosporidium antibody, clone BEL 0126 recognizes a membrane antigen expressed by the oocysts of Cryptosporidium sp., an obligate enteric coccidian parasite of the phylum Apicomplexa that infects the gastrointestinal tract. The parasite is one of the most important enteric pathogens in both humans and animals (Rose et al. 2002). Since its first diagnosis in 1975 (Meisel et al. 1976), Cryptosporidiosis, has become one of the most prominent public health concerns worldwide (Rose et al. 2002).

Cryptosporidium oocysts are resistant to chlorine and their small size makes removal by filtration difficult. Alternative methods have been developed such as UV and ozone treatment alongside monitoring using Immunofluorescence screening (Rose et al. 2002).

Cryptosporidiosis is a disease affecting the intestines of mammals which is spread through the fecal-oral route. The main symptom of is self-limiting diarrhea in people with intact immune systems. However, in immunocompromised individuals, such as AIDS patients, infection can cause permanent & life-threatening diarrhea (Ma et al. 1984).

Product Details

Target Species
Protozoan
Product Form
Purified IgG conjugated to Alexa Fluor®488 - liquid
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Product Form
Purified IgG - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by affinity chromatography on Protein G
Preparation
Purified IgG prepared by affinity chromatography on Protein A
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine serum albumin
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
1%Bovine serum albumin
Preservative Stabilisers
0.09%Sodium Azide (NaN3)
Carrier Free
Yes
Immunogen
Purified cryptosporidium oocysts from bovine faeces.
Approx. Protein Concentrations
IgG concentration 0.05mg/ml
Approx. Protein Concentrations
IgG concentration 0.1mg/ml
Approx. Protein Concentrations
1.0 mg/ml

Storage Information

Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Storage
Store at +4oC or at -20oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.
Shelf Life
18 months from date of despatch.

More Information

Acknowledgements
This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchase product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
Regulatory
For research purposes only

Applications of Cryptosporidium antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry 1/5 1/20
Flow Cytometry 1/5 1/50
Immunofluorescence
ELISA 1/2000 1/8000
Flow Cytometry
Immunofluorescence 1/50 1/200
Immunohistology - Paraffin
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using the appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.

Secondary Antibodies Available

Description Product Code Pack Size Applications List Price Quantity
Goat anti Mouse IgG (H/L):HRP (Multi Species Adsorbed) STAR117P 0.5 mg E WB
Rabbit F(ab')2 anti Mouse IgG:FITC STAR9B 1 mg F

Product Specific References

References for Cryptosporidium antibody

  1. Mansfield, K.G. et al. (1997) Identification of an Enterocytozoon bieneusi-like microsporidian parasite in simian-immunodeficiency-virus-inoculated macaques with hepatobiliary disease.
    Am J Pathol. 150: 1395-405.
  2. Weigum, S.E. et al. (2016) Hollow silica microspheres for buoyancy-assisted separation of infectious pathogens from stool.
    J Chromatogr A. 1466: 29-36.

Further Reading

  1. Meisel, J.L. et al. (1976) Overwhelming watery diarrhea associated with a cryptosporidium in an immunosuppressed patient.
    Gastroenterology. 70: 1156-60.
  2. Rose, J.B. et al. (2002) Risk and control of waterborne cryptosporidiosis.
    FEMS Microbiol Rev. 26: 113-23.
  3. Ma, P. (1984) Cryptosporidium and the enteropathy of immune deficiency.
    J Pediatr Gastroenterol Nutr. 3 (4): 488-90.