PARP1-S499-ADP-RIBOSE antibody | AbD34251

anti PARP1-S499-ADP-RIBOSE

Product Type
Monoclonal Antibody
Clone
AbD34251
Isotype
IgG
Specificity
PARP1-S499-ADP-RIBOSE

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HCA356
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Anti-PARP1-S499-ADP-ribose antibody, clone AbD34251, recognizes PARP1, ADP-ribosylated at Serine 499. Poly(ADP-ribose) polymerase 1 (PARP1) is an early responder to DNA damage in human cells. Upon binding to genomic lesions PARP1 is able to transfer a mono- or poly-ADP-ribose residue from nicotinamide adenine dinucleotide (NAD+) to their substrate proteins, for chromatin decompaction and repair factor recruitment (Bütepage et al. 2015, Suskiewicz et al. 2020).

During the DNA damage response, serine is the primary target for PARP1 ADP-ribosylation which uses Histone PARylation factor 1 (HPF1) as an accessory factor to switch the amino-acid specificity of PARP1 from aspartate/glutamate to serine residues (Bonfiglio et al. 2017, Suskiewicz et al. 2020). PARP1/HPF1 during DNA damage response, primarily target PARP1 itself and histone H3, ribosylating these substrate proteins.

This PARP1-S499ADPr antibody, clone AbD34251 was generated using an ADP-ribosylated PARP1S499 antigen. Characterization has demonstrated the antibody does not bind any other Ser-ADP-ribosylated peptides tested and binds via three residues before and one after the modified Serine. This clone does not detect poly-ADP-ribose in a site-specific manner (Bonfiglio et al. 2020).

Target Species
Protein/peptide tag
Product Form
Human/Rabbit IgG chimera (rabbit CH2 and CH3) antibody selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
Preparation
Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line.
Buffer Solution
Phosphate buffered saline.
Preservative Stabilisers
0.01% Thiomersal.
Carrier Free
Yes.
Immunogen
APRGKS(ADPr)GAALSKKSKGQVGGK
Approx. Protein Concentrations
Total protein concentration 0.5 mg/ml.
Regulatory
For research purposes only.
Guarantee
12 months from date of despatch.
Acknowledgements
This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA
Western Blotting 2 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Antibody Characterization Reference

  1. Bonfiglio, J.J. et al. (2020) An HPF1/PARP1-Based Chemical Biology Strategy for Exploring ADP-Ribosylation.
    Cell. 183 (4): 1086-1102.e23. Cell. 183 (4): 1086-1102.e23.