PARP1-S499-ADP-RIBOSE antibody | AbD34251
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- Product Type
- Monoclonal Antibody
|Anti-PARP1-S499-ADP-ribose antibody, clone AbD34251, recognizes PARP1, ADP-ribosylated at Serine 499. Poly(ADP-ribose) polymerase 1 (PARP1) is an early responder to DNA damage in human cells. Upon binding to genomic lesions PARP1 is able to transfer a mono- or poly-ADP-ribose residue from nicotinamide adenine dinucleotide (NAD+) to their substrate proteins, for chromatin decompaction and repair factor recruitment (Bütepage et al. 2015, Suskiewicz et al. 2020).
During the DNA damage response, serine is the primary target for PARP1 ADP-ribosylation which uses Histone PARylation factor 1 (HPF1) as an accessory factor to switch the amino-acid specificity of PARP1 from aspartate/glutamate to serine residues (Bonfiglio et al. 2017, Suskiewicz et al. 2020). PARP1/HPF1 during DNA damage response, primarily target PARP1 itself and histone H3, ribosylating these substrate proteins.
This PARP1-S499ADPr antibody, clone AbD34251 was generated using an ADP-ribosylated PARP1S499 antigen. Characterization has demonstrated the antibody does not bind any other Ser-ADP-ribosylated peptides tested and binds via three residues before and one after the modified Serine. This clone does not detect poly-ADP-ribose in a site-specific manner (Bonfiglio et al. 2020).
- Target Species
- Protein/peptide tag
- Product Form
- Human/Rabbit IgG chimera (rabbit CH2 and CH3) antibody selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Carrier Free
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml
- For research purposes only
- 12 months from date of despatch
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
|Western Blotting||2 ug/ml|
Antibody Characterization Reference
Bonfiglio, J.J. et al. (2020) An HPF1/PARP1-Based Chemical Biology Strategy for Exploring ADP-Ribosylation.
Cell. 183 (4): 1086-1102.e23. Cell. 183 (4): 1086-1102.e23.
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