PARP1-S499-ADP-RIBOSE antibody | AbD34251
During the DNA damage response, serine is the primary target for PARP1 ADP-ribosylation which uses Histone PARylation factor 1 (HPF1) as an accessory factor to switch the amino-acid specificity of PARP1 from aspartate/glutamate to serine residues (Bonfiglio et al. 2017, Suskiewicz et al. 2020). PARP1/HPF1 during DNA damage response, primarily target PARP1 itself and histone H3, ribosylating these substrate proteins.
This PARP1-S499ADPr antibody, clone AbD34251 was generated using an ADP-ribosylated PARP1S499 antigen. Characterization has demonstrated the antibody does not bind any other Ser-ADP-ribosylated peptides tested and binds via three residues before and one after the modified Serine. This clone does not detect poly-ADP-ribose in a site-specific manner (Bonfiglio et al. 2020).
- Target Species
- Protein/peptide tag
- Product Form
- Human/Rabbit IgG chimera (rabbit CH2 and CH3) antibody selected from the HuCAL® phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Carrier Free
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
- 12 months from date of despatch
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
- For research purposes only
Applications of PARP1-S499-ADP-RIBOSE antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Western Blotting||2 ug/ml|
Product Specific References
Antibody Characterization Reference
Bonfiglio, J.J. et al. (2020) An HPF1/PARP1-Based Chemical Biology Strategy for Exploring ADP-Ribosylation.
Cell. 183 (4): 1086-1102.e23.
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