Mono-ADP-Ribose antibody | AbD33204
(Image courtesy of Dr Ivan Matic, Max Planck Institute for Biology of Aging)
(Image courtesy of Dr Ivan Matic, Max Planck Institute for Biology of Aging)
Anti mono-ADP-ribose antibody, clone AbD33204 (HCA354) used to detect mono-ADP-ribose by western blotting.
Image caption:
SpyTag-based modular antibodies enable the sensitive and versatile detection of mono-ADPr
(B) Mono-ADPr immunoblotting with AbD33204 or AbD43647.
(C) Specificity of AbD43647 by immunoblotting.
(D) Immunoprecipitation and immunoblotting of histones with AbD43647 (top panel). Histone levels by Ponceau staining (bottom panel).
From: Longarini EJ, Dauben H, Locatelli C, Wondisford AR, Smith R, Muench C, Kolvenbach A, Lynskey ML, Pope A, Bonfiglio JJ, Jurado EP, Fajka-Boja R, Colby T, Schuller M, Ahel I, Timinszky G, O'Sullivan RJ, Huet S, Matic I.
Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.
Mol Cell. 2023 May 18;83(10):1743-1760.e11.
doi: 10.1016/j.molcel.2023.03.027.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
anti Mono-ADP-Ribose
- Product Type
- Monoclonal Antibody
- Clone
- AbD33204
- Isotype
- IgG
- Specificity
- Mono-ADP-Ribose
Antibody Quality and Performance Guarantee
| Anti mono-ADP-ribose antibody, clone AbD33204, recognizes mono-adenosine diphosphate (ADP)-ribosylation. ADP-ribosylation is a reversible post-translational modification that occurs in multicellular organisms as well as some lower unicellular eukaryotes, but is absent in prokaryotes and yeast (Bürkle 2005). ADP ribosylation has been shown to play critical roles in many physiological and pathological processes, including bacterial pathogenesis and signaling and metabolism to control chromatin-related processes including transcription and DNA repair (Bonfiglio et al. 2020, Bütepage et al. 2015). Members of the ADP-ribosyltransferase (ART) superfamily of proteins including the poly(ADP-ribose) polymerases (PARPs) subfamily, catalyze the transfer of ADP-ribose from nicotinamide adenine dinucleotide (NAD+) onto substrate protein via N-, O-, or S-glycosidic linkages. These transferases are able to transfer a single ADP-ribose residue to their substrate proteins, in a process known as mono-ADP-ribosylation (Bütepage et al. 2015). This clone AbD33204 is a mono-selective ADP-ribose antibody which was generated using an H3S10ADPr peptide as the antigen. This clone is specific for mono-ADPr and has a preference for ubiquitin Arg-ADPr and mono-ADPr catalyzed by other PARPs (Bonfiglio et al. 2020). Another mono-ADPr antibody (HCA355, AbD33205) also specifically recognizes mono-ADP-ribose, however it has a preference for Ser-mono-ADP-riboses. |
- Target Species
- Protein/peptide tag
- Product Form
- Human/Rabbit IgG chimera (rabbit CH2 and CH3) antibody selected from the HuCAL phage display library and expressed in a human cell line. This antibody is supplied as a liquid.
- Preparation
- Purified recombinant IgG prepared by affinity chromatography on Protein A from a mammalian cell line
- Source
- HKB-11
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
- Carrier Free
- Yes
- Immunogen
- ARTKQTARKS(ADPr)TGGKAC
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml
- Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA |  |
||
| Immunofluorescence | |
1/100 | |
| Western Blotting | |
2 ug/ml |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| anti Mono-ADP-Ribose | HCA355 | IF WB | 0.1 mg |
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| Description | anti Mono-ADP-Ribose | ||||||
Antibody Characterization Reference
-
Bonfiglio, J.J. et al. (2020) An HPF1/PARP1-Based Chemical Biology Strategy for Exploring ADP-Ribosylation.
Cell. 183 (4): 1086-1102.e23.
References for Mono-ADP-Ribose antibody
-
Longarini, E.J. et al. (2023) Modular antibodies reveal DNA damage-induced mono-ADP-ribosylation as a second wave of PARP1 signaling.
Mol Cell. 83 (10): 1743-1760.e11.
HCA354
158439
160362
162452
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