H3-S10-ADP-RIBOSE antibody | AbD33644
Poly(ADP-ribose) polymerase 1 (PARP1) is an early responder to DNA damage in human cells. Upon binding to genomic lesions PARP1 is able to transfer a mono- or poly-ADP-ribose residue from nicotinamide adenine dinucleotide (NAD+) to their substrate proteins, for chromatin decompaction and repair factor recruitment (Bütepage et al. 2015, Suskiewicz et al. 2020).
During the DNA damage response, serine is the primary target for PARP1 ADP-ribosylation which uses Histone PARylation factor 1 (HPF1) as an accessory factor to switch the amino-acid specificity of PARP1 from aspartate/glutamate to serine residues (Bonfiglio et al. 2017, Suskiewicz et al. 2020). During the DNA damage response PARP1 and HPF1 primarily target PARP1 itself and histone H3, ribosylating these targets.
This clone has been used in Western blotting, in the context of the DNA damage response with Histone H3 Ser-ADPr sites recognized by this antibody in lysates from Wild Type and ARH3 KO cells treated with H2O2 (Bonfiglio et al. 2020).
- Target Species
- Protein/peptide tag
- Product Form
- A bivalent human recombinant Fab (lambda light chain) selected from the HuCAL phage display library. Expressed in E. coli. This Fab fragment is bivalent by dimerization of the bacterial alkaline phosphatase fusion protein. The antibody is tagged with a DYKDDDDK tag and a HIS-tag (HHHHHH) at the C-terminus of the antibody heavy chain. This antibody is supplied as liquid.
- Metal chelate affinity chromatography
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.01% Thiomersal
- Carrier Free
- Approx. Protein Concentrations
- Total protein concentration 0.5 mg/ml
- This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. Should this product contain a precipitate we recommend microcentrifugation before use.
- 12 months from date of despatch
- Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
His-tag is a registered trademark of EMD Biosciences.
- For research purposes only
Applications of H3-S10-ADP-RIBOSE antibody
|Application Name||Verified||Min Dilution||Max Dilution|
|Western Blotting||2 ug/ml|
Product Specific References
Antibody Characterization Reference
Bonfiglio, J.J. et al. (2020) An HPF1/PARP1-Based Chemical Biology Strategy for Exploring ADP-Ribosylation.
Cell. 183 (4): 1086-1102.e23.
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