Siglec-10 antibody | 2E9
Mouse anti Pig Siglec-10:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- 2E9
- Isotype
- IgG1
- Specificity
- Siglec-10
Antibody Quality and Performance Guarantee
MCA6169A647
| Mouse anti Pig Siglec-10, clone 2E9 recognizes Siglec-10. Sialic-acid-binding immunoglobulin-like lectin (Siglecs) are cell surface receptors belonging to the immunoglobulin superfamily and recognize terminal sialic acids present in complex oligosaccharides of glycoproteins or glycolipids. Siglecs are mainly expressed on the cells of the immune systems and play a regulatory role, modulating inflammatory and immune responses. When expressed on transfected cells, porcine Siglec-10 is able to bind red blood cells in a sialic acid-dependent manner. Mouse anti Pig Siglec-10 antibody, clone 2E9 has been successfully used to examine Siglec-10 cell and tissue distribution in swine. Siglec-10 was found to be expressed on blood B cells (CD21+ or CD79a+) and in B cell areas of the spleen and lymph nodes. Weak expression was also detected on monocytes (Escalona et al. 2014). |
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin - Immunogen
- NIH/3T3 mouse cells transfected with plasmid pSiglec-10-GFP, encoding the porcine Siglec-10 tagged with the green fluorescent protein (GFP) on the carboxy-terminal end
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 | ||||||
Source Reference
-
Escalona, Z. et al. (2015) Molecular characterization of porcine Siglec-10 and analysis of its expression in blood and tissues.
Dev Comp Immunol. 48 (1): 116-23.
References for Siglec-10 antibody
-
Álvarez-Estrada, Á. et al. (2019) TLR2, Siglec-3 and CD163 expressions on porcine peripheral blood monocytes are increased during sepsis caused by Haemophilus parasuis.
Comp Immunol Microbiol Infect Dis. 64: 31-39. -
Poderoso, T. et al. (2019) Analysis of the expression of porcine CD200R1 and CD200R1L by using newly developed monoclonal antibodies.
Dev Comp Immunol. 100: 103417. -
Poderoso, T. et al. (2020) Expression of Siglec-1, -3, -5 and -10 in porcine cDC1 and cDC2 subsets from blood, spleen and lymph nodes and functional capabilities of these cells.
Dev Comp Immunol. 109: 103692. -
Álvarez, B. et al. (2023) Porcine Macrophage Markers and Populations: An Update.
Cells. 12 (16): 2103.
MCA6169A647
165473
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