Endothelial Cells antibody | MIL11
Mouse anti Pig Endothelial Cells antibody, clone MIL11 (MCA1752) used for the identification of porcine jujenal vasculature by immunofluorescence.
Image caption:
Colonisation expands IgM and IgA-producing mucosal plasma cells. (A) IgA staining in jejunal mucosa from a GF pig and (B) a colonised pig at 21 days of age. Sections were stained with antibodies to IgA (red) and capillary endothelium (MIL11; in blue). In both (A) and (B), (i) shows the two-colour image; (ii) shows the original greyscale image for the red channel (IgA); (iii) shows the original greyscale image for the blue channel (endothelium). Scale bar represents 10 μm. (C) IgM staining in jejunal mucosa from a GF pig and (D) a colonised pig at 21 days of age. Sections were stained with antibodies to IgM (red). Scale bar represents 10 μm. (E) Area of IgM and (F) IgA staining in jejunal sections from piglets at birth (open diamonds) and GF piglets (open squares) and colonised piglets (black squares) at 5 and 21days of age (see Table 1 for numbers of piglets in each experiment). * p<0.01.
From: Inman CF, Laycock GM, Mitchard L, Harley R, Warwick J, Burt R, et al. (2012)
Neonatal Colonisation Expands a Specific Intestinal Antigen-Presenting Cell Subset Prior to CD4 T-Cell Expansion, without Altering T-Cell Repertoire.
PLoS ONE 7(3): e33707.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Pig Endothelial Cells antibody, clone MIL11 (MCA1752) used for the identification of porcine jujenal vasculature by immunofluorescence.
Image caption:
Colonisation expands mucosal CD4+ T cells by 21 days of age. (A) CD4 staining in jejunal mucosa from a GF pig and (B) a colonised pig at 21 days of age. Sections were stained with antibodies to CD4 (green) and capillary endothelium (MIL11; in blue). In both (A) and (B), (i) shows the two-colour image; (ii) shows the original greyscale image for the blue channel (endothelium); (iii) shows the original greyscale image for the green channel (CD4). Scale bar represents 10 μm. (C) Area of CD4+ T cell staining in jejunal sections from piglets at birth (open diamonds) and GF piglets (open squares) and colonised piglets (black squares) at 5 and 21 days of age (see Table 1 for numbers of piglets in each experiment). *p^lt;0.05.
From: Inman CF, Laycock GM, Mitchard L, Harley R, Warwick J, Burt R, et al. (2012) Neonatal Colonisation Expands a Specific Intestinal Antigen-Presenting Cell Subset Prior to CD4 T-Cell Expansion, without Altering T-Cell Repertoire.
PLoS ONE 7(3): e33707.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Pig Endothelial Cells antibody, clone MIL11 (MCA1752) used for the identification of endothelium in porcine melanoma by immunofluorescence.
Image caption:
Expression of collagen IV in swine melanoma. (A) 3-week-old pig. (B) 10-week-old pig. (C) 3-month-old pig. (D) 8-month-old pig. Green: collagen IV; blue: melanin; red: blood vessel walls (A, C) and nuclei (B, D). Bar=20 μm.
From: Planska D, Burocziova M, Strnadel J, Horak V. Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig.
Acta Histochem Cytochem. 2015;48(1):15-26.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Pig Endothelial Cells antibody, clone MIL11 (MCA1752) used for the identification of endothelium in porcine melanoma by immunofluorescence.
Image caption:
Expression of collagen IV in swine melanoma. (A) 3-week-old pig. (B) 10-week-old pig. (C) 3-month-old pig. (D) 8-month-old pig. Green: collagen IV; blue: melanin; red: blood vessel walls (A, C) and nuclei (B, D). Bar=20 μm.
From: Planska D, Burocziova M, Strnadel J, Horak V. Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig.
Acta Histochem Cytochem. 2015;48(1):15-26.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Porcine endothelial cell antibosy, clone MIL11 (MCA1752) used for the evaluation of endothelial cell presence in porcine camples by immunofluorescence.
Image caption:
Effects of neonatal and early-life environments on expression of antigen-presenting cell-associated molecules in the intestinal mucosa. Quantitative analysis of proportion positive pixels by fluorescence immunohistology of proximal (E) and distal (F) jejunal lamina propria at 5, 28, and 56 days old in the following treatment groups (n = 6/treatment/timepoint): outdoor extensive farm reared; outdoor extensive origin, isolator reared; outdoor extensive origin, isolator reared with antibiotics; indoor intensive farm-reared; indoor intensive origin isolator reared; indoor intensive-origin isolator reared with antibiotics MIL11 (capillary endothelium) (E,F).
From: Christoforidou Z, Burt R, Mulder I, Gill BP, Pluske J, Kelly D, Stokes CR, Bailey M, Lewis MC.
Development of Immune Cells in the Intestinal Mucosa Can Be Affected by Intensive and Extensive Farm Environments, and Antibiotic Use.
Front Immunol. 2018 May 16;9:1061.
doi: 10.3389/fimmu.2018.01061.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Porcine endothelial cell antibosy, clone MIL11 (MCA1752) used for the evaluation of endothelial cell presence in porcine camples by immunofluorescence.
Image caption:
Effects of neonatal and early-life environments on expression of antigen-presenting cell-associated molecules in the intestinal mucosa.Representational images (I) show positive staining for MIL11 (blue) within the intestinal mucosa at the specified sites in piglets from the groups indicated at 5, 28, and 56 days.
From: Christoforidou Z, Burt R, Mulder I, Gill BP, Pluske J, Kelly D, Stokes CR, Bailey M, Lewis MC.
Development of Immune Cells in the Intestinal Mucosa Can Be Affected by Intensive and Extensive Farm Environments, and Antibiotic Use.
Front Immunol. 2018 May 16;9:1061.
doi: 10.3389/fimmu.2018.01061.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Porcine endothelial cell antibosy, clone MIL11 (MCA1752) used for the evaluation of endothelial cell presence in porcine camples by immunofluorescence.
Image caption:
Antigen presentation within the intestinal lamina propria is complex. Representation of fluorescence immunohistology from the distal jejunum lamina propria of a 28-day-old isolator-reared piglet. (A) CD14 (red), CD16 (intestinal dendritic cells) (green), MIL11 (capillary endothelium) (blue), and MHCII DR (white). (B) combined image of antigen presentation-associated molecule s CD16 (green), MIL11 (blue), MHCII (red), CD16 and MIL11 co-staining (cyan), CD16 and MHCII co-staining (yellow), MIL11 and MHCII DR co-staining (magenta), and CD16, MIL11, and MHCII DR co-staining (white).
From: Christoforidou Z, Burt R, Mulder I, Gill BP, Pluske J, Kelly D, Stokes CR, Bailey M, Lewis MC.
Development of Immune Cells in the Intestinal Mucosa Can Be Affected by Intensive and Extensive Farm Environments, and Antibiotic Use.
Front Immunol. 2018 May 16;9:1061.
doi: 10.3389/fimmu.2018.01061.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Porcine endothelium antibody, clone MIL11 (MCA1752) used for the demonstration of endothelium in porcine colon sections by immunofluorescence.
Image caption:
Representational images of CD4 (a), CD16 (bone-marrow-derived APC) (b), MIL11 (intestinal epithelium) (c) and MHCIIDR (d) staining in colonic tissue of a control piglet. Representational image of co-staining with CD16 (red), MIL11 (green) and MHCIIDR (blue) (e) and co-staining with CD4 (red), CD16 (green) and MIL11 (blue) (f).
From: Lewis MC, Merrifield CA, Berger B, Cloarec O, Duncker S, Mercenier A, Nicholson JK, Holmes E, Bailey M.
Early intervention with Bifidobacterium lactis NCC2818 modulates the host-microbe interface independent of the sustained changes induced by the neonatal environment.
Sci Rep. 2017 Jul 13;7(1):5310.
doi: 10.1038/s41598-017-05689-z.
This image is from an open access article distributed under the terms of the Creative Commons Attribution License.
Mouse anti Pig endothelial cells, clone MIL11 raised initially against an immunogen of plastic adherent lymphocytes has been shown to recognize a major subset of porcine endothelial cells. MIL11 was initially identified as staining a characteristic pattern of capillary endothelium in gut forming a network in the lamina propria below the epithelial membrane. Staining was subsequently observed in capillary endothelium in most organs examined, including gut, lung, kidney and skin. Clone MIL11 was observed not to stain endothelium of aorta and other arteries whereas venous endothelium was positive for MIL11 staining. Some population of venous endothelial cells however appear negative for MIL11 staining including those associated with the heart wall, kidney glomeruli and corpus cavernosum (Wilson et al.1996) .
Product Details
- Target Species
- Pig
- Product Form
- Tissue Culture Supernatant - liquid
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
0.09% Sodium Azide - Immunogen
- Plastic adherent porcine peripheral blood lymphocytes.
Storage Information
- Storage
- Store at +4oC or at -20oC if preferred.
This product should be stored undiluted.
Storage in frost free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. - Guarantee
- 18 months from date of despatch.
More Information
- Regulatory
- For research purposes only
Applications of Endothelial Cells antibody
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Immunohistology - Frozen | 1/20 | 1/100 |
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.
Copyright © 2019 Bio-Rad Antibodies (formerly AbD Serotec)
Product Specific References
References for Endothelial Cells antibody
-
Wilson, A. D. et al. (1996) Expression of major histocompatibility complex class II antigens, on normal porcine intestinal endothelium.
Immunology 88: 98-103. -
Harrower, T.P. et al. (2006) Long-term survival and integrationof porcine expanded neural precursor cell grafts in a rat model of Parkinson's disease.
Exp Neurol.197: 56-69. -
Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.
J Leukoc Biol. 88 (3): 571-8. -
Inman, C.F. et al. (2010) Rearing environment affects development of the immune system in neonates.
Clin Exp Immunol. 160: 431-9. -
Cho, P.S. et al. (2008) Immunogenicity of umbilical cord tissue derived cells.
Blood. 111: 430-8. -
Inman, C.F. et al. (2012) Neonatal colonisation expands a specific intestinal antigen-presenting cell subset prior to CD4 T-cell expansion, without altering T-cell repertoire.
PLoS One. 7: e33707. -
Planska, D. et al. (2015) Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig.
Acta Histochem Cytochem. 48 (1): 15-26. -
Christoforidou, Z. et al. (2018) Development of Immune Cells in the Intestinal Mucosa Can Be Affected by Intensive and Extensive Farm Environments, and Antibiotic Use.
Front Immunol. 9: 1061. -
Lewis, M.C. et al. (2017) Early intervention with Bifidobacterium lactis NCC2818 modulates the host-microbe interface independent of the sustained changes induced by the neonatal environment.
Sci Rep. 7 (1): 5310.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
