Endothelial Cells antibody | MIL11

Mouse anti Pig Endothelial Cells

Product Type
Monoclonal Antibody
Clone
MIL11
Isotype
IgE
Specificity
Endothelial Cells

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MCA1752
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Mouse anti Pig endothelial cells, clone MIL11 raised initially against an immunogen of plastic adherent lymphocytes has been shown to recognize a major subset of porcine endothelial cells. MIL11 was initially identified as staining a characteristic pattern of capillary endothelium in gut forming a network in the lamina propria below the epithelial membrane. Staining was subsequently observed in capillary endothelium in most organs examined, including gut, lung, kidney and skin. Clone MIL11 was observed not to stain endothelium of aorta and other arteries whereas venous endothelium was positive for MIL11 staining. Some population of venous endothelial cells however appear negative for MIL11 staining including those associated with the heart wall, kidney glomeruli and corpus cavernosum (Wilson et al.1996) .

Target Species
Pig
Product Form
Tissue Culture Supernatant - liquid
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09%Sodium Azide
Immunogen
Plastic adherent porcine peripheral blood lymphocytes.
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Immunohistology - Frozen 1/20 1/100
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls.

References for Endothelial Cells antibody

  1. Wilson, A. D. et al. (1996) Expression of major histocompatibility complex class II antigens, on normal porcine intestinal endothelium.
    Immunology 88: 98-103.
  2. Harrower, T.P. et al. (2006) Long-term survival and integrationof porcine expanded neural precursor cell grafts in a rat model of Parkinson's disease.
    Exp Neurol.197: 56-69.
  3. Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.
    J Leukoc Biol. 88 (3): 571-8.
  4. Inman, C.F. et al. (2010) Rearing environment affects development of the immune system in neonates.
    Clin Exp Immunol. 160: 431-9.
  5. Cho, P.S. et al. (2008) Immunogenicity of umbilical cord tissue derived cells.
    Blood. 111 (1): 430-8.
  6. Inman, C.F. et al. (2012) Neonatal colonisation expands a specific intestinal antigen-presenting cell subset prior to CD4 T-cell expansion, without altering T-cell repertoire.
    PLoS One. 7: e33707.
  7. Planska, D. et al. (2015) Immunohistochemical Analysis of Collagen IV and Laminin Expression in Spontaneous Melanoma Regression in the Melanoma-Bearing Libechov Minipig.
    Acta Histochem Cytochem. 48 (1): 15-26.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Immunofluorescence

RRID
AB_322717
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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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