CLEC12B antibody | PELE6

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Mouse anti Pig CLEC12B:Alexa Fluor® 647

Product Type: Monoclonal Antibody
Clone: PELE6
Isotype: IgG1
Specificity: CLEC12B

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Storage Information
Negative Controls
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

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MCA6446A647 Datasheet SDS SDS	F	100 Tests/1ml		Log in
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Anti Pig CLEC12B Antibody, clone PELE6 thumbnail image 1

Anti Pig CLEC12B Antibody, clone PELE6 thumbnail image 2

Anti Pig CLEC12B Antibody, clone PELE6 thumbnail image 3

Anti Pig CLEC12B Antibody, clone PELE6 gallery image 1 — Pig alveolar macrophages were stained with Anti-Porcine CD172a A647 (MCA2312A647) (Figure A) or with Anti-Porcine CD172a A647 (MCA2312A647) and Anti-Porcine CLEC12B RPE (**MCA6446PE**) (Figure B). Data were acquired on the ZE5 Cell Analyzer.

Anti Pig CLEC12B Antibody, clone PELE6 gallery image 2 — Pig alveolar macrophages were stained with Anti-Porcine CD172a RPE (MCA2312PE) (Figure A) or with Anti-Porcine CD172a RPE (MCA2312PE) and Anti-Porcine CLEC12B A647 (**MCA6446A647**) (Figure B). Experiments were performed on pig alveolar macrophages gated on live, single cells. Data were acquired on the ZE5 Cell Analyzer.

Anti Pig CLEC12B Antibody, clone PELE6 gallery image 3 — Pig alveolar macrophages were stained with Anti-Porcine CD14 A647 (MCA1218A647) and Anti-Mouse IgG1 isotype control (MCA928) (Figure A) or with Anti-Porcine CD14 A647 (MCA1218A647) and Anti-Porcine CLEC12B (**MCA6446GA**) (Figure B) detected with Anti-Mouse IgG1 FITC (STAR132F). Experiments were performed on pig alveolar macrophages gated on live, single cells. Data were acquired on the ZE5 Cell Analyzer.

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Mouse anti-Pig CLEC12B antibody, clone PELE6 recognizes pig CLEC12B.

CLEC12B is a C-type lectin-like receptor expressed on myeloid cells, playing a role in immune regulation and pathogen recognition. In pigs, CLEC12B is prominently expressed on alveolar macrophages and, to a lesser extent, on myeloid dendritic cells and plasmacytoid dendritic cells. CLEC12B is not expressed on monocytes, monocyte-derived macrophages, and monocyte-derived dendritic cells.

Mouse anti-Pig CLEC12B antibody, clone PELE6, has been instrumental in characterizing the expression of CLEC12B on porcine alveolar macrophages and dendritic cells. This antibody has played a crucial role in characterizing CLEC12B expression and examining its function in immune responses, particularly in the context of microbial infections and vaccination strategies. Mouse anti-Pig CLEC12B binds and is efficiently internalized in dendritic cells without significantly altering cytokine secretion, making it a valuable tool for studying antigen delivery and vaccine efficacy in porcine models (Nieto-Pelegrín et al. 2020).

Target Species: Pig
Product Form: Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
Approx. Protein Concentrations: IgG concentration 0.05 mg/ml
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

Alexa Fluor®647 650 665
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product is sold under a license from Spanish National Research Council (CSIC). This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		Neat	1/5

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10ul of the suggested working dilution to label 1x10⁶ cells in 100ul

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Description	Product Code	Applications	Pack Size	Your Price
Mouse IgG1 Negative Control:Alexa Fluor® 647	MCA928A647	F	100 Tests/1ml	Log in
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Description	Mouse IgG1 Negative Control:Alexa Fluor® 647