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CD33 antibody | 5D5

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Anti Pig CD33 Antibody, clone 5D5 thumbnail image 1
Anti Pig CD33 Antibody, clone 5D5 gallery image 1
Figure A. FITC conjugated Mouse anti Pig CD14 (MCA1218F) and Alexa Fluor 647 conjugated Mouse IgG1 isotype control (MCA928A647). Figure B. FITC conjugated Mouse anti Pig CD14 (MCA1218F) and Alexa Fluor 647 conjugated Mouse anti Pig CD33 (MCA6167A647). All experiments performed on frozen porcine blood gated on single mononuclear cells, in the presence of 10% pig serum. Data acquired on the ZE5 Cell Analyzer.

Mouse anti Pig CD33:FITC

Product Type
Monoclonal Antibody
Clone
5D5
Isotype
IgG1
Specificity
CD33

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Product Code Applications Pack Size List Price Your Price Qty
MCA6167F
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
List Price Your Price
loader

Mouse anti Pig CD33, clone 5D5 recognizes CD33, also known as Siglec-3.

Sialic-acid-binding immunoglobulin-like lectin (Siglecs) are cell surface receptors belonging to the immunoglobulin superfamily and recognize terminal sialic acids present in complex oligosaccharides of glycoproteins or glycolipids. Siglecs are mainly expressed on the cells of the immune systems and play a regulatory role, modulating inflammatory and immune responses.

Clone 5D5 was used to detect Siglec-3 expression on monocytes (CD172a+ or CD16hi PBMCs), granulocytes (CD52+ and CD52-), and bone marrow. CD33 was also detected in lymph node, splenic, and alveolar macrophages, although at lower levels than on monocytes (Alvarez et al. 2015).

Target Species
Pig
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
Recombinant protein consisting in the two extracellular Ig-like domains of porcine Siglec-3 fused to the Fc portion of human IgG1 (pSiglec- 3-Fc)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/5
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10ul of the suggested working dilution to label 1x106 cells in 100ul

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
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List Price Your Price
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Description Mouse IgG1 Negative Control:FITC

Source Reference

  1. Álvarez, B. et al. (2015) Molecular and functional characterization of porcine Siglec-3/CD33 and analysis of its expression in blood and tissues.
    Dev Comp Immunol. 51 (2): 238-50.

References for CD33 antibody

  1. Bohorquez, J.A. et al. (2019) Identification of an Immunosuppressive Cell Population during Classical Swine Fever Virus Infection and Its Role in Viral Persistence in the Host.
    Viruses. 11 (9): 822.
  2. Álvarez-Estrada, Á. et al. (2019) TLR2, Siglec-3 and CD163 expressions on porcine peripheral blood monocytes are increased during sepsis caused by Haemophilus parasuis.
    Comp Immunol Microbiol Infect Dis. 64: 31-39.
  3. Poderoso, T. et al. (2020) Expression of Siglec-1, -3, -5 and -10 in porcine cDC1 and cDC2 subsets from blood, spleen and lymph nodes and functional capabilities of these cells.
    Dev Comp Immunol. 109: 103692.
  4. Álvarez, B. et al. (2023) Porcine Macrophage Markers and Populations: An Update.
    Cells. 12 (16): 2103.

MCA6167F

163328 165437

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Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

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