Browsing Modes for Business Users

CD25 antibody | K231.3B2

ISO 9001 Quality Assurance Manufacturing | Print
Anti Pig CD25 Antibody, clone K231.3B2 thumbnail image 1 Anti Pig CD25 Antibody, clone K231.3B2 thumbnail image 2 Anti Pig CD25 Antibody, clone K231.3B2 thumbnail image 3 Anti Pig CD25 Antibody, clone K231.3B2 thumbnail image 4 Anti Pig CD25 Antibody, clone K231.3B2 thumbnail image 5
Anti Pig CD25 Antibody, clone K231.3B2 gallery image 1
Porcine peripheral blood lymphocytes stained with Mouse anti Pig CD25 antibody, clone K231.3B2 (MCA1736GA) followed by FITC conjugated Goat anti Mouse IgG antibody (STAR117F), dual stained with RPE conjugated Mouse anti Pig CD4a antibody, clone MIL17 (MCA1749PE). Numbers indicate the percent cells in each quadrant.
Anti Pig CD25 Antibody, clone K231.3B2 gallery image 2
Published customer image:
Mouse anti Pig CD25, clone K231.3B2 (MCA1736) used for the evaluation of CD25 expression on lymphocytes in culture by flow cytometry.
Image caption:
Induction of Tregs from lymphocytes co-cultured with PRRSV-infected MoDCs. (A) Representative flow cytometry profile of lymphocytes following 3 days co-culture of PBMCs with PRRSV-infected MoDCs (upper layer), and only culture with PRRSV-infected PBMCs (lower layer). (B) Percentage of Foxp3+ cells in the gated CD4+CD25+ subpopulations of PBMCs co-cultured with PRRSV-infected MoDC. (C) Percentage of Foxp3+ cells in the gated CD4+ CD25+ subpopulations of PRRSV-infected PBMCs alone. (D) Sorted CD4+CD25high cells. (E) Percentage suppression at the indicated Tregs: peripheral lymphocyte ratios. The lymphocytes exposed to Marc-145 lysate-treated MoDCs were used as negative control. The percentage of suppression was calculated as follows: % suppression = 100 × [1 − (% proliferation w/PRRSV/ % proliferation w/mock)] [35]. Data from three independent experiments. All data analysis was done using one-way ANOVA and significant differences are shown (*P<0.05 and **P< 0.01).

From: Fan B, Liu X, Bai J, Li Y, Zhang Q, Jiang P (2015) The 15N and 46R Residues of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Protein Enhance Regulatory T Lymphocytes Proliferation.
PLoS ONE 10(9): e0138772.
Under a Creative Commons license
Anti Pig CD25 Antibody, clone K231.3B2 gallery image 3
Figure A. FITC conjugated mouse anti porcine CD4a (MCA1749F) and purified mouse IgG1 isotype control (MCA928) detected with goat anti mouse IgG1PE (STAR132PE). Figure B. FITC conjugated mouse anti porcine CD4a (MCA1749F) and purified mouse anti porcine CD25 (MCA1736GA) detected with goat anti mouse IgG1PE (STAR132PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Anti Pig CD25 Antibody, clone K231.3B2 gallery image 4
Figure A. Purified mouse anti porcine CD25 (MCA1736GA) detected with goat anti mouse IgG1 PE (STAR132PE) and mouse IgG2b FITC isotype control (MCA691F). Figure B. Purified mouse anti porcine CD25 (MCA1736GA) detected with goat anti mouse IgG1 PE (STAR132PE) and mouse anti porcine CD4a FITC (MCA1749F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Anti Pig CD25 Antibody, clone K231.3B2 gallery image 5
Published customer image:
Mouse anti Pig CD25, clone K231.3B2 (MCA1736GA) used to identify CD25 expressing cells in porcine lamina propria by immunofluorescence.
Image caption:
Representation of 3 color fluorescence immunohistology of CD4 (red), CD25 (blue), Foxp3 (green), and CD4CD25 (magenta) positive staining in the distal jejunum lamina propria of a 28 day old female and male control (no dietary supplementation) piglets (A). Bar = 10 μm.

From: Christoforidou Z, Mora Ortiz M, Poveda C, Abbas M, Walton G, Bailey M, Lewis MC.
Sexual Dimorphism in Immune Development and in Response to Nutritional Intervention in Neonatal Piglets.
Front Immunol. 2019 Dec 9;10:2705.
doi: 10.3389/fimmu.2019.02705.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

Mouse anti Pig CD25:StarBright Violet 610

Product Type
Monoclonal Antibody
Clone
K231.3B2
Isotype
IgG1
Specificity
CD25

Quick Links:

 Antibody Quality and Performance Guarantee
 Unrivaled Technical Support
Product Code Applications Pack Size List Price Your Price Qty
MCA1736SBV610
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 100 Tests/0.5ml loader
List Price Your Price
loader

Mouse anti Pig CD25, clone K231.3B2 recognizes porcine CD25, the alpha chain of the interleukin 2 receptor (IL-2Rα), also known as the low affinity Interleukin 2 receptor. The IL-2 receptor exists in three forms, the high affinity heterodimer, the intermediate affinity β monomer and the low affinity α monomer configurations. Clone K231.3B2 was clustered as CD25 at the First International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Lunney et al. 1994).

Mouse anti pig CD25, clone K231.3B2 immunoprecipitates a protein of ~65-70 kDa from activated lymphocyte preparations (Bailey et al. 1992).

CD25 is a 270 amino acid single pass type I transmembrane glycoprotein containing 2 Sushi domains. Low expression of CD25 is seen on resting peripheral blood mononuclear cells, rapidly up-regulated following stimulation by concanavalin A and phorbol myristate acetate, indicative of its role as an activation antigen (Bullido et al. 1999).

Target Species
Pig
Product Form
Purified IgG conjugated to StarBright Violet 610 - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen
Con A activated porcine peripheral blood lymphocytes.
Fusion Partners
Spleen cells from immunized mice were fused with cells of the mouse P3-X63-Ag.8.653 myeloma cell line.
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
StarBright Violet 610 403 607
Regulatory
For research purposes only
Guarantee
12 months from date of despatch
Acknowledgements
This product is covered by U.S. Patent No. 10,150,841 and related U.S. and foreign counterparts

Store at +4°C. DO NOT FREEZE.
This product should be stored undiluted.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 5μl of the suggested working dilution to label 106 cells in 100μl. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application.

How to Use the Spectraviewer

Watch the Tool Tutorial Video ▸
  • Start by selecting the application you are interested in, with the option to select an instrument from the drop down menu or create a customized instrument
  • Select the fluorophores or fluorescent proteins you want to include in your panel to check compatibility
  • Select the lasers and filters you wish to include
  • Select combined or multi-laser view to visualize the spectra

References for CD25 antibody

  1. Bailey, M. et al. (1992) A monoclonal antibody recognising an epitope associated with pig interleukin-2 receptors.
    J Immunol Methods. 153 (1-2): 85-91.
  2. Barker, E. et al. (2006) The larynx as an immunological organ: immunological architecture in the pig as a large animal model.
    Clin Exp Immunol. 143: 6-14.
  3. Silva-Campa, E. et al. (2009) Induction of T helper 3 regulatory cells by dendritic cells infected with porcine reproductive and respiratory syndrome virus.
    Virology. 387: 373-9.
  4. Silva-Campa, E. et al. (2010) European genotype of porcine reproductive and respiratory syndrome (PRRSV) infects monocyte-derived dendritic cells but does not induce Treg cells.
    Virology. 396 (2): 264-71.
  5. Kick, A.R. et al. (2011) Evaluation of peripheral lymphocytes after weaning and vaccination for Mycoplasma hyopneumoniae.
    Res Vet Sci. 91 (3): e68-72.
  6. LeRoith, T. et al. (2011) A modified live PRRSV vaccine and the pathogenic parent strain induce regulatory T cells in pigs naturally infected with Mycoplasma hyopneumoniae.
    Vet Immunol Immunopathol. 140 (3-4): 312-6.
  7. Kuo, Y.R. et al. (2011) Prolongation of composite tissue allotransplant survival by treatment with bone marrow mesenchymal stem cells is correlated with T-cell regulation in a swine hind-limb model.
    Plast Reconstr Surg. 127: 569-79.
  8. Young, D. et al. (2012) Soy-derived di- and tripeptides alleviate colon and ileum inflammation in pigs with dextran sodium sulfate-induced colitis.
    J Nutr. 142: 363-8.
    9. View The Latest Product References
  9. Chattha, K.S. et al. (2013) Divergent immunomodulating effects of probiotics on T cell responses to oral attenuated human rotavirus vaccine and virulent human rotavirus infection in a neonatal gnotobiotic piglet disease model.
    J Immunol. 191: 2446-56.
  10. Fan, B. et al. (2015) The 15N and 46R Residues of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Protein Enhance Regulatory T Lymphocytes Proliferation.
    PLoS One. 10 (9): e0138772.
  11. Singleton, H. et al. (2016) Establishing Porcine Monocyte-Derived Macrophage and Dendritic Cell Systems for Studying the Interaction with PRRSV-1.
    Front Microbiol. 7: 832.
  12. Ferrari, L. et al. (2016) Phenotypic modulation of porcine CD14+ monocytes, natural killer/natural killer T cells and CD8αβ+ T cell subsets by an antibody-derived killer peptide (KP).
    Res Vet Sci. 109: 29-39.
  13. Suradhat, S. et al. (2016) Transdermal delivery of plasmid encoding truncated nucleocapsid protein enhanced PRRSV-specific immune responses.
    Vaccine. 34 (5): 609-15.
  14. Williams, A.R. et al. (2016) Polymerization-dependent activation of porcine γδ T-cells by proanthocyanidins.
    Res Vet Sci. 105: 209-15.
  15. Pan, H. et al. (2016) Lymphodepletive effects of rabbit anti-pig thymocyte globulin in neonatal swines.
    Transpl Immunol. 39: 74-83.
  16. Sirisereewan, C. et al. (2017) Positive immunomodulatory effects of heterologous DNA vaccine- modified live vaccine, prime-boost immunization, against the highly-pathogenic PRRSV infection.
    Vet Immunol Immunopathol. 183: 7-15.
  17. An, C.H. et al. (2018) Plant synthetic GP4 and GP5 proteins from porcine reproductive and respiratory syndrome virus elicit immune responses in pigs.
    Planta. 247 (4): 973-85.
  18. Nedumpun, T. et al. (2019) Negative Immunomodulatory Effects of Type 2 Porcine Reproductive and Respiratory Syndrome Virus-Induced Interleukin-1 Receptor Antagonist on Porcine Innate and Adaptive Immune Functions.
    Front Immunol. 10: 579.
  19. Christoforidou, Z. et al. (2019) Sexual Dimorphism in Immune Development and in Response to Nutritional Intervention in Neonatal Piglets.
    Front Immunol. 10: 2705.
  20. Uehlein, S. et al. (2021) Human-like Response of Pig T Cells to Superagonistic Anti-CD28 Monoclonal Antibodies.
    J Immunol .ji2100174.
  21. Schäfer, A. et al. (2021) T-cell responses in domestic pigs and wild boar upon infection with the moderately virulent African swine fever virus strain 'Estonia2014'.
    Transbound Emerg Dis. 68 (5): 2733-49.
  22. Monguió-Tortajada, M. et al. (2022) Acellular cardiac scaffolds enriched with MSC-derived extracellular vesicles limit ventricular remodelling and exert local and systemic immunomodulation in a myocardial infarction porcine model.
    Theranostics. 12 (10): 4656-4670.
  23. Nielsen, O.L. et al. (2022) A porcine model of subcutaneous Staphylococcus aureus infection: a pilot study.
    APMIS. 130 (7): 359-70.
  24. Maciag, S.S. et al. (2022) On the influence of the source of porcine colostrum in the development of early immune ontogeny in piglets.
    Sci Rep. 12 (1): 15630.
  25. Haach, V. et al. (2023) A polyvalent virosomal influenza vaccine induces broad cellular and humoral immunity in pigs.
    Virol J. 20 (1): 181.
  26. Bettin, L. et al. (2023) Co-stimulation by TLR7/8 ligand R848 modulates IFN-γ production of porcine γδ T cells in a microenvironment-dependent manner.
    Dev Comp Immunol. 138: 104543.
  27. Shimazu, T. et al. (2019) Addition of Wakame seaweed (Undaria pinnatifida) stalk to animal feed enhances immune response and improves intestinal microflora in pigs.
    Anim Sci J. 90 (9): 1248-60.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.

Immunofluorescence

Synonyms
IL-2R Alpha Chain
RRID
AB_2943386
UniProt
O02733
Entrez Gene
IL2RA
GO Terms
GO:0016021 integral to membrane
GO:0004872 receptor activity

MCA1736SBV610

100008126

If you cannot find the batch/lot you are looking for please contact our technical support team for assistance.

View more products with CD25 specificity

Please Note: All Products are "FOR RESEARCH PURPOSES ONLY"

View all Anti-Pig Products
Please note