CD169 antibody | 3B11/11
Mouse anti Porcine CD169 antibody, clone 3B11/11 (MCA2316GA) used for the evaluation of CD169 expression on porcine cells by flow cytometry and immunohistochemistry.
Image caption:
(A) MAb 1F1 and 3B11 recognize an antigen expressed in alveolar macrophages and MoDC. Cells were labeled with 1F1 or 3B11 mAb (black histograms) or with irrelevant isotype-matched mAb (grey histograms). Results are representative of five independent experiments. (B) In the spleen, staining with 1F1 (1) and 3B11 (2) mAb appears associated with the walls of the ellipsoidal vessels that surround the islands of white pulp. (C) In lymph nodes, the antigen was detected in the subcapsular sinus and medullary cords (1) and in the periphery of follicles and some follicular cells (2).
From: Revilla C, Poderoso T, Martínez P, Alvarez B, López-Fuertes L, Alonso F, Ezquerra A, Domínguez J.
Targeting to porcine sialoadhesin receptor improves antigen presentation to T cells.
Vet Res. 2009 May-Jun;40(3):14.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Porcine CD169 antibody, clone 3B11/11 (MCA2316) used for the evaluation of CD169 expression on porcine cells by western blotting and immunoprecipitation.
Image caption:
MAb 1F1 and 3B11 bind to two different epitopes on the Sn molecule. Black histograms show binding of labeled 3B11 or 1F1 mAb on alveolar macrophages after incubation with the indicated unlabeled antibodies. Grey histograms correspond to the negative control staining. Results are representative of at least two independent experiments using cells from different animals.
From: Revilla C, Poderoso T, Martínez P, Alvarez B, López-Fuertes L, Alonso F, Ezquerra A, Domínguez J.
Targeting to porcine sialoadhesin receptor improves antigen presentation to T cells.
Vet Res. 2009 May-Jun;40(3):14.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Porcine CD169 antibody, clone 3B11/11 (MCA2316GA) used for the evaluation of epitope binding in comparison to clone 1F1 by flow cytometry on porcine alveolar macrophages.
Image caption:
MAb 1F1 and 3B11 bind to two different epitopes on the Sn molecule. Black histograms show binding of labeled 3B11 or 1F1 mAb on alveolar macrophages after incubation with the indicated unlabeled antibodies. Grey histograms correspond to the negative control staining. Results are representative of at least two independent experiments using cells from different animals.
From: Revilla C, Poderoso T, Martínez P, Alvarez B, López-Fuertes L, Alonso F, Ezquerra A, Domínguez J.
Targeting to porcine sialoadhesin receptor improves antigen presentation to T cells.
Vet Res. 2009 May-Jun;40(3):14.
This is from an open access article distributed under the terms of a Creative Commons Attribution License.
Mouse anti Pig CD169:Alexa Fluor® 647
- Product Type
- Monoclonal Antibody
- Clone
- 3B11/11
- Isotype
- IgG1
- Specificity
- CD169
Antibody Quality and Performance Guarantee
| Mouse anti Pig CD169, clone 3B11/11 recognizes porcine CD169, also known as sialoadhesin or Siglec-1, a member of the sialic acid binding immunoglobulin-like lectin (Siglec) family. CD169 was originally identified in mice and identified as the sialic acid dependent Sheep erythrocyte receptor (Crocker et al.1986). CD169 has subsequently been identified in rat (van den Berg et al. 1992), human (Mucklow et al. 1995) and pig (Vanderheijden et al. 2003) . Mouse anti Porcine CD169, clone 3B1/11 was originally raised as part of a panel of anti porcine macrophage monoclonal antibodies raised against isolated porcine alveolar macrophages (Bullido et al. 1997). Immunohistochemical analysis indicated restriction to macrophage populations mainly in the spleen, lymph nodes, liver and Peyer’s patches. Originally described as a non phagocytic intercellular adhesion receptor, work on porcine CD169 indicated that it may play a role as a viral adhesion receptor (Delputte et al. 2006) and as a targeted receptor for the delivery of toxins and antigens (Delputte et al. 2011) . Mouse anti pig CD169, clone 3B11/11 detects a band of approximately 190 kDa in alveolar macrophage extracts under non-reducing conditions (Revilla et al 2009). |
- Target Species
- Pig
- Product Form
- Purified IgG conjugated to Alexa Fluor® 647 - liquid
- Preparation
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin - Immunogen
- Porcine alveolar macrophages.
- Approx. Protein Concentrations
- IgG concentration 0.05 mg/ml
- Fusion Partners
- Spleen cells from immunized BALB/c mice were fused with cells of the mouse X63-Ag.8.653 myeloma cell line.
- Max Ex/Em
-
Fluorophore Excitation Max (nm) Emission Max (nm) Alexa Fluor®647 650 665 - Regulatory
- For research purposes only
- Guarantee
- 12 months from date of despatch
- Acknowledgements
- This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| Flow Cytometry |  |
Neat | 1/10 |
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
- Flow Cytometry
- Use 10μl of the suggested working dilution to 1x106 cells in 100μl
| Description | Product Code | Applications | Pack Size | List Price | Your Price | Quantity | |
|---|---|---|---|---|---|---|---|
| Mouse IgG1 Negative Control:Alexa Fluor® 647 | MCA928A647 | F | 100 Tests/1ml |
|
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| List Price | Your Price | ||||||
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| Description | Mouse IgG1 Negative Control:Alexa Fluor® 647 | ||||||
Source Reference
-
Bullido, R. et al. (1997) Monoclonal antibodies specific for porcine monocytes/macrophages: macrophage heterogeneity in the pig evidenced by the expression of surface antigens.
Tissue Antigens. 49 (4): 403-13.
References for CD169 antibody
-
Thacker, E. et al. (2001) Summary of workshop findings for porcine myelomonocytic markers.
Vet Immunol Immunopathol. 80 (1-2): 93-109. -
Perdiguero, B. & Blasco, R. (2006) Interaction between vaccinia virus extracellular virus envelope A33 and B5 glycoproteins.
J Virol. 80 (17): 8763-77. -
Perdiguero, B. et al. (2008) Vaccinia virus A34 glycoprotein determines the protein composition of the extracellular virus envelope.
J Virol. 82 (5): 2150-60. -
Revilla, C. et al. (2009) Targeting to porcine sialoadhesin receptor improves antigen presentation to T cells.
Vet Res. 40 (3): 14. -
Ezquerra, A. et al. (2009) Porcine myelomonocytic markers and cell populations.
Dev Comp Immunol. 33 (3): 284-98. -
Prather, R.S. et al. (2013) An Intact Sialoadhesin (Sn/SIGLEC1/CD169) Is Not Required for Attachment/Internalization of the Porcine Reproductive and Respiratory Syndrome Virus.
J Virol. 87: 9538-46. -
Costa-Hurtado, M. et al. (2013) Changes in macrophage phenotype after infection of pigs with Haemophilus parasuis strains with different levels of virulence.
Infect Immun. 81 (7): 2327-33. -
Rodríguez-Gómez IM et al. (2015) PRRSV-infected monocyte-derived dendritic cells express high levels of SLA-DR and CD80/86 but do not stimulate PRRSV-naïve regulatory T cells to proliferate.
Vet Res. 46: 54.
View The Latest Product References
-
Whitworth, K.M. et al. (2016) Gene-edited pigs are protected from porcine reproductive and respiratory syndrome virus.
Nat Biotechnol. 34 (1): 20-2. -
Singleton, H. et al. (2016) Establishing Porcine Monocyte-Derived Macrophage and Dendritic Cell Systems for Studying the Interaction with PRRSV-1.
Front Microbiol. 7: 832. -
Burkard, C. et al. (2017) Precision engineering for PRRSV resistance in pigs: Macrophages from genome edited pigs lacking CD163 SRCR5 domain are fully resistant to both PRRSV genotypes while maintaining biological function.
PLoS Pathog. 13 (2): e1006206. -
Wells, K.D. et al. (2017) Replacement of Porcine CD163 Scavenger Receptor Cysteine-Rich Domain 5 with a CD163-Like Homolog Confers Resistance of Pigs to Genotype 1 but Not Genotype 2 Porcine Reproductive and Respiratory Syndrome Virus.
J Virol. 91 (2): pii: e01521-16. -
Chen, J. et al. (2019) Generation of Pigs Resistant to Highly Pathogenic-Porcine Reproductive and Respiratory Syndrome Virus through Gene Editing of CD163.
Int J Biol Sci. 15 (2): 481-492. -
Li, P. et al. (2020) Susceptibility of porcine pulmonary microvascular endothelial cells to porcine reproductive and respiratory syndrome virus.
J Vet Med Sci. 82 (9): 1404-9. -
Álvarez, B. et al. (2023) Porcine Macrophage Markers and Populations: An Update.
Cells. 12 (16) :2103.
Further Reading
-
Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
Vet Res. 39: 54.
- Synonyms
- Sialoadhesin
- UniProt
- A7LCJ3
- Entrez Gene
- SIGLEC-1
- GO Terms
- GO:0005886 plasma membrane
- GO:0007155 cell adhesion
- GO:0016021 integral to membrane
- GO:0005529 sugar binding
- GO:0006897 endocytosis
MCA2316A647
152567
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