CD16 antibody | G7
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|Mouse anti Pig CD16, clone G7 recognizes porcine CD16 also known as Fc-gamma RIII or the low affinity IgG (Fc) receptor III. Clone G7 was clustered as CD16 at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Saalmuller et al. 1998).
Mouse anti pig CD16 immunoprecipitates a protein of ~40 kDa from porcine neutrophils and NK cells (Wierda et al. 1993). Subsequent cloning and characterization of the G7 molecule indicated that G7 was the porcine homologue of Human CD16 (Halloran et al. 1994).
- Target Species
- Product Form
- Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
- Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% sodium azide (NaN3)
1% bovine serum albumin
- Porcine peripheral blood leucocytes
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Spleen cells from immunised Balb/c mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line
- Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm) FITC 490 525
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.
|Application Name||Verified||Min Dilution||Max Dilution|
- Flow Cytometry
- Use 10μl of the suggested working dilution to label 106 cells in 100μl
References for CD16 antibody
Dato, M.E. et al. (1992) A triggering structure recognized by G7 monoclonal antibody on porcine lymphocytes and granulocytes.
Cell Immunol. 140 (2): 468-77.
Wierda, W.G. et al. (1993) Two distinct porcine natural killer lytic trigger molecules as PNK-E/G7 molecular complex.
Cell Immunol. 146 (2): 270-83.
Halloran, P.J. et al. (1994) Biochemical characterization of the porcine Fc gamma RIII alpha homologue G7.
Cell Immunol. 158 (2): 400-13.
Devriendt, B. et al. (2010) Targeting of Escherichia coli F4 fimbriae to Fcgamma receptors enhances the maturation of porcine dendritic cells.
Vet Immunol Immunopathol. 135 (3-4): 188-98.
Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.
J Leukoc Biol. 88 (3): 571-8.
Terzic, S. et al. (2002) Immunophenotyping of leukocyte subsets in peripheral blood and palatine tonsils of prefattening pigs.
Vet Res Commun. 26: 273 - 83.
Masure, D. et al. (2013) A Role for Eosinophils in the Intestinal Immunity against Infective Ascaris suum Larvae.
PLoS Negl Trop Dis. 7: e2138.
Hester, S.N. et al. (2012) Intestinal and systemic immune development and response to vaccination are unaffected by dietary (1,3/1,6)-β-D-glucan supplementation in neonatal piglets.
Clin Vaccine Immunol. 19 (9): 1499-508.
View The Latest Product References
Kapetanovic, R. et al. (2012) Pig bone marrow-derived macrophages resemble human macrophages in their response to bacterial lipopolysaccharide.
J Immunol. 188: 3382 - 94.
Gimeno, M. et al. (2011) Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.
Vet Res. 42: 9.
Mussá, T. et al. (2011) Interaction of porcine conventional dendritic cells with swine influenza virus.
Virology 420: 125-34.
Vincent, I.E. et al. (2003) Dendritic cells harbor infectious porcine circovirus type 2 in the absence of apparent cell modulation or replication of the virus.
J Virol. 77: 13288 - 300.
Inman, C.F. et al. (2012) Neonatal colonisation expands a specific intestinal antigen-presenting cell subset prior to CD4 T-cell expansion, without altering T-cell repertoire.
PLoS One 7: e33707.
Sánchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.
J Immunol. 162 (9): 5230-7.
Lecours, M.P. et al. (2011) Characterization of porcine dendritic cell response to Streptococcus suis.
Vet Res. 42: 72.
Inman, C.F. et al. (2010) Rearing environment affects development of the immune system in neonates.
Clin Exp Immunol. 160 (3): 431-9.
Summerfield, A. et al. (2003) Porcine peripheral blood dendritic cells and natural interferon-producing cells.
Immunology 110: 440-9.
Mair, K.H. et al. (2012) NKp46 expression discriminates porcine NK cells with different functional properties.
Eur J Immunol. 42: 1261-71.
Mair, K.H. et al. (2013) Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.
Vet Res. 44: 13.
Auray, G. et al. (2016) Characterization and Transcriptomic Analysis of Porcine Blood Conventional and Plasmacytoid Dendritic Cells Reveals Striking Species-Specific Differences.
J Immunol. Nov 11. pii: 1600672. [Epub ahead of print]
Kyrova, K. et al. (2014) The response of porcine monocyte derived macrophages and dendritic cells to Salmonella typhimurium and lipopolysaccharide.
BMC Vet Res. 10: 244.
Suzuki, S. et al. (2016) Generation and characterization of RAG2 knockout pigs as animal model for severe combined immunodeficiency.
Vet Immunol Immunopathol. 178: 37-49.
Waide, E.H. et al. (2015) Not All SCID Pigs Are Created Equally: Two Independent Mutations in the Artemis Gene Cause SCID in Pigs.
J Immunol. 195 (7): 3171-9.
Loss, H. et al. (2018) Effects of a pathogenic ETEC strain and a probiotic Enterococcus faecium strain on the inflammasome response in porcine dendritic cells.
Vet Immunol Immunopathol. 203: 78-87.
LeLuduec, J.B. et al. (2016) Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.
Vaccine. 34 (7): 914-22.
Ferret-Bernard, S. et al. (2020) Maternal Supplementation of Food Ingredient (Prebiotic) or Food Contaminant (Mycotoxin) Influences Mucosal Immune System in Piglets.
Nutrients. 12 (7): 2115.
Skovdal, S.M. et al. (2019) Inhaled nebulized glatiramer acetate against Gram-negative bacteria is not associated with adverse pulmonary reactions in healthy, young adult female pigs.
PLoS One. 14 (10): e0223647.
Fernández-Caballero, T. et al. (2018) Phenotypic and functional characterization of porcine bone marrow monocyte subsets.
Dev Comp Immunol. 81: 95-104.
Teuben, M.P.J. et al. (2021) Standardized porcine unilateral femoral nailing is associated with changes in PMN activation status, rather than aberrant systemic PMN prevalence.
Eur J Trauma Emerg Surg. Jun 10 [Epub ahead of print].
Teuben, M. et al. (2021) Instant intra-operative neutropenia despite the emergence of banded (CD16dim/CD62Lbright) neutrophils in peripheral blood - An observational study during extensive trauma-surgery in pigs.
Injury. 52 (3): 426-33.
Van der Weken, H. et al. (2021) Antibody-Mediated Targeting of Antigens to Intestinal Aminopeptidase N Elicits Gut IgA Responses in Pigs.
Front Immunol. 12: 753371.
Boettcher, A.N. et al. (2020) CD3ε+ Cells in Pigs With Severe Combined Immunodeficiency Due to Defects in ARTEMIS
Frontiers in Immunology. 11 [Epub ahead of print].
Zhao, H. et al. (2022) Development of RAG2 -/- IL2Rγ -/Y immune deficient FAH-knockout miniature pig.
Front Immunol. 13: 950194.
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