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CD16 antibody | G7

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Anti Pig CD16 Antibody, clone G7 thumbnail image 1 Anti Pig CD16 Antibody, clone G7 thumbnail image 2 Anti Pig CD16 Antibody, clone G7 thumbnail image 3 Anti Pig CD16 Antibody, clone G7 thumbnail image 4 Anti Pig CD16 Antibody, clone G7 thumbnail image 5 Anti Pig CD16 Antibody, clone G7 thumbnail image 6 Anti Pig CD16 Antibody, clone G7 thumbnail image 7 Anti Pig CD16 Antibody, clone G7 thumbnail image 8
Anti Pig CD16 Antibody, clone G7 gallery image 1
Figure A. Alexa Fluor 647 conjugated mouse anti porcine CD45 (MCA1222A647) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. Alexa Fluor 647 conjugated mouse anti porcine CD45 (MCA1222A647) and FITC conjugated mouse anti porcine CD16 (MCA1971F). All experiments performed on red cell lysed porcine blood gated on lymphocytes. Data acquired on the ZE5 Cell Analyzer.
Anti Pig CD16 Antibody, clone G7 gallery image 2
Figure A. FITC conjugated mouse anti porcine CD45 (MCA1222F) and PE conjugated mouse IgG1 isotype control (MCA928PE). Figure B. FITC conjugated mouse anti porcine CD45 (MCA1222F) and PE conjugated mouse anti porcine CD16 (MCA1971PE). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Anti Pig CD16 Antibody, clone G7 gallery image 3
Figure A. PE conjugated mouse anti porcine CD16 (MCA1971PE) and FITC conjugated mouse IgG1 isotype control (MCA928F). Figure B. PE conjugated mouse anti porcine CD16 (MCA1971PE) and FITC conjugated mouse anti porcine CD45 (MCA1222F). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Anti Pig CD16 Antibody, clone G7 gallery image 4
Published customer image:
Mouse anti Porcine CD16 antibody, clone G7 (MCA1971GA used to identify CD16 expressing cells in porcine jejenum by immunofluorescence.
Image caption:
Colonisation affects mucosal MHCII+ APC subsets.
(A) Area of MHCII+ cell staining in jejunal sections from piglets at birth (open diamonds) and GF piglets (open squares) and colonised piglets (black squares) at 5 and 21 days of age (see Table 1 for numbers of piglets in each experiment). (B) SIRPα staining in jejunal mucosa from a GF pig and (C) a colonised pig at 21 days of age. (B) (i) and (C) (i) show staining with antibodies to CD16 (red), SIRPα (green) and CD11R1 (blue). (B) (ii) and (C) (ii) show the same sections with the same cells in red and blue but with MHCII DR in green. In both (B) and (C), (iii) shows the red channel (SIRPα); (iv) shows the green channel (CD16), (v) shows the blue channel (CD11R1); (vi) shows the infra-red channel (MHC class II). Scale bar represents 10 μm. (D) Partitioning of MHCII between SIRPα+ (green shading) and SIRPα− (purple shading) APC subsets in the intestines of pigs at birth and from both treatment groups at 5 days and 21 days of age (see Table 1 for numbers of piglets in each experiment).

From: Inman CF, Laycock GM, Mitchard L, Harley R, Warwick J, Burt R, et al. (2012)
Neonatal Colonisation Expands a Specific Intestinal Antigen-Presenting Cell Subset Prior to CD4 T-Cell Expansion, without Altering T-Cell Repertoire.
PLoS ONE 7(3): e33707.
This is from an open access article distributed under the terms of the Creative Commons Attribution License.
Anti Pig CD16 Antibody, clone G7 gallery image 5
Published customer image:
FITC conjugated Mouse anti Pig CD16, clone G7 (MCA1971F) used to label porcine APCs by flow cytometry.
Image caption:
Influence of F18 fimbriae on antigen presenting capacity of intestinal antigen presenting cells. (A) Phenotypical analysis of small intestinal lamina propria mononuclear cells (LP MC). LP MCs were stained to determine CD172a, CD16 and MHCII expression in the live cell gate (CytoxBlue neg.) upon doublet discrimination. R1: lymphocytes; R6: MHCII+ activated T- and B-cells; R5: CD3+ T cells; R2-R4: myeloid cells. Representative plots for five separate experiments. (B) The LP MC population R6 consists mainly of IgM+ B-cells, while CD3+ T-cells make up the R5 population. (C) Morphological differences between CD16hi and CD16+ populations. (D) Macrophage-specific staining (CD68; red) of CD16hi and CD16+ population. Cell nuclei are shown in blue (Hoechst staining).

From: Van der Weken H, Sanz Garcia R, Sanders NN, Cox E, Devriendt B.
Antibody-Mediated Targeting of Antigens to Intestinal Aminopeptidase N Elicits Gut IgA Responses in Pigs.
Front Immunol. 2021 Oct 14;12:753371.
doi: 10.3389/fimmu.2021.753371.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Pig CD16 Antibody, clone G7 gallery image 6
Published customer image:
RPE conjugated Mouse anti Pig CD16 antibody, clone G7 (MCCA1971PE) used for the phenotyping of porcine PBMCs by flow cytometry.
Image caption:
The expression of ADGRE1 on blood leukocytes in the pig. Flow cytometry was carried out on pig PBMCs (A–H) and alveolar macrophages (I). Representative flow cytometry images are illustrated (n = 3) for live cells (identified by exclusion using Sytox blue). Where shown, quadrant gates were set using isotype matched control antibody staining. Peripheral blood mononuclear cells were examined for expression of ADGRE1 using monoclonal antibody ROS-4E12-3E6 (A) and positive cells (red) were identified. Multiple color flow cytometry was used to determine the co-expression of ADGRE1 with CD14 (B), CD16 (C), and CD163 (D) on PBMCs. Expression of ADGRE1 with CD14 (E), CD16 (F), and CD163 (G) was also determined in PBMC from pigs treated in-vivo with pCSF1-Fc treatment. The mean percentage ADGRE1 positive cells ± SE is shown comparing control pigs (B–D) with pCSF1-Fc treated pigs (E–G). *P <0.05 by Welch's t-test; n = 3 pigs per treatment. Note that these samples are a subset of a larger cohort from our previous study (27) in which CSF1-Fc was shown to increase the blood monocyte count (P <0.0001). (H) Histogram of ADGRE1 median fluorescence intensity in PBMC from pCSF1-Fc treated pigs (red), control animals (black), and isotype controls (blue and green for control and treated respectively); n = 3. Expression of ADGRE1 was measured on CD172a+ macrophages from bronchoalveolar lavage (I); n = 3.

From: Waddell LA, Lefevre L, Bush SJ, Raper A, Young R, Lisowski ZM, McCulloch MEB, Muriuki C, Sauter KA, Clark EL, Irvine KM, Pridans C, Hope JC, Hume DA.
ADGRE1 (EMR1, F4/80) Is a Rapidly-Evolving Gene Expressed in Mammalian Monocyte-Macrophages.
Front Immunol. 2018 Oct 1;9:2246.
doi: 10.3389/fimmu.2018.02246.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.
Anti Pig CD16 Antibody, clone G7 gallery image 7
Figure A. StarBright Violet 475 conjugated Mouse anti Porcine CD3 (​MCA5951SBV475). Figure B. StarBright Violet 475 conjugated Mouse anti Porcine CD3 (​MCA5951SBV475) and StarBright Violet 790 conjugated  Mouse anti Porcine CD16 (​MCA1971SBV790). All experiments performed on red cell lysed porcine blood gated on live single lymphocytes, in the presence of 10% porcine serum. Data acquired on the ZE5 Cell Analyzer.
Anti Pig CD16 Antibody, clone G7 gallery image 8
Published customer image:
AlexaFluor 647 conjugated Mouse anti Pig CD16 antibody, clone G7 (MCA1971A647) used to label myeloid cells in pig blood by flow cytometry.
Image caption:
Confirmation of scRNA-seq cluster annotation by flow cytometry. Annotation of clusters in scRNA-seq was confirmed by flow cytometry using antibody panels addressing key marker combinations. Colors used in UMAP clustering (left) match with colored gates and histograms in flow cytometry to indicate the same cell type (right). (A) Different subsets of myeloid cells.

From: Muir A, Paudyal B, Schmidt S, Sedaghat-Rostami E, Chakravarti S, Villanueva-Hernández S, Moffat K, Polo N, Angelopoulos N, Schmidt A, Tenbusch M, Freimanis G, Gerner W, Richard AC, Tchilian E.
Single-cell analysis reveals lasting immunological consequences of influenza infection and respiratory immunization in the pig lung.
PLoS Pathog. 2024 Jul 18;20(7):e1011910.
doi: 10.1371/journal.ppat.1011910.
This image is from an open access article distributed under terms of a Creative Commons Attribution License.

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Mouse anti Pig CD16:FITC

Product Type
Monoclonal Antibody
Clone
G7
Isotype
IgG1
Specificity
CD16

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Product Code Applications Pack Size List Price Your Price Qty
MCA1971F
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F 0.1 mg loader
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Mouse anti Pig CD16, clone G7 recognizes porcine CD16 also known as Fc-gamma RIII or the low affinity IgG (Fc) receptor III. Clone G7 was clustered as CD16 at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Saalmuller et al. 1998).

Mouse anti pig CD16 immunoprecipitates a protein of ~40 kDa from porcine neutrophils and NK cells (Wierda et al. 1993). Subsequent cloning and characterization of the G7 molecule indicated that G7 was the porcine homologue of Human CD16 (Halloran et al. 1994).

Target Species
Pig
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
Porcine peripheral blood leucocytes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

How to Use the Spectraviewer

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
List Price Your Price
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Description Mouse IgG1 Negative Control:FITC

Source Reference

  1. Dato, M.E. et al. (1992) A triggering structure recognized by G7 monoclonal antibody on porcine lymphocytes and granulocytes.
    Cell Immunol. 140 (2): 468-77.

References for CD16 antibody

  1. Wierda, W.G. et al. (1993) Two distinct porcine natural killer lytic trigger molecules as PNK-E/G7 molecular complex.
    Cell Immunol. 146 (2): 270-83.
  2. Halloran, P.J. et al. (1994) Biochemical characterization of the porcine Fc gamma RIII alpha homologue G7.
    Cell Immunol. 158 (2): 400-13.
  3. Sánchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.
    J Immunol. 162 (9): 5230-7.
  4. Terzic, S. et al. (2002) Immunophenotyping of leukocyte subsets in peripheral blood and palatine tonsils of prefattening pigs.
    Vet Res Commun. 26: 273 - 83.
  5. Vincent, I.E. et al. (2003) Dendritic cells harbor infectious porcine circovirus type 2 in the absence of apparent cell modulation or replication of the virus.
    J Virol. 77: 13288 - 300.
  6. Summerfield, A. et al. (2003) Porcine peripheral blood dendritic cells and natural interferon-producing cells.
    Immunology 110: 440-9.
  7. Inman, C.F. et al. (2010) Rearing environment affects development of the immune system in neonates.
    Clin Exp Immunol. 160 (3): 431-9.
  8. Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.
    J Leukoc Biol. 88 (3): 571-8.
    9. View The Latest Product References
  9. Devriendt, B. et al. (2010) Targeting of Escherichia coli F4 fimbriae to Fcgamma receptors enhances the maturation of porcine dendritic cells.
    Vet Immunol Immunopathol. 135 (3-4): 188-98.
  10. Gimeno, M. et al. (2011) Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.
    Vet Res. 42: 9.
  11. Mussá, T. et al. (2011) Interaction of porcine conventional dendritic cells with swine influenza virus.
    Virology 420: 125-34.
  12. Lecours, M.P. et al. (2011) Characterization of porcine dendritic cell response to Streptococcus suis.
    Vet Res. 42: 72.
  13. Mair, K.H. et al. (2012) NKp46 expression discriminates porcine NK cells with different functional properties.
    Eur J Immunol. 42: 1261-71.
  14. Hester, S.N. et al. (2012) Intestinal and systemic immune development and response to vaccination are unaffected by dietary (1,3/1,6)-β-D-glucan supplementation in neonatal piglets.
    Clin Vaccine Immunol. 19 (9): 1499-508.
  15. Thierry, A. et al. (2012) Identification of invariant natural killer T cells in porcine peripheral blood.
    Vet Immunol Immunopathol. 149 (3-4): 272-9.
  16. Inman, C.F. et al. (2012) Neonatal colonisation expands a specific intestinal antigen-presenting cell subset prior to CD4 T-cell expansion, without altering T-cell repertoire.
    PLoS One 7: e33707.
  17. Masure, D. et al. (2013) A Role for Eosinophils in the Intestinal Immunity against Infective Ascaris suum Larvae.
    PLoS Negl Trop Dis. 7: e2138.
  18. Mair, K.H. et al. (2013) Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.
    Vet Res. 44: 13.
  19. Kyrova, K. et al. (2014) The response of porcine monocyte derived macrophages and dendritic cells to Salmonella typhimurium and lipopolysaccharide.
    BMC Vet Res. 10: 244.
  20. Waide, E.H. et al. (2015) Not All SCID Pigs Are Created Equally: Two Independent Mutations in the Artemis Gene Cause SCID in Pigs.
    J Immunol. 195 (7): 3171-9.
  21. Auray, G. et al. (2016) Characterization and Transcriptomic Analysis of Porcine Blood Conventional and Plasmacytoid Dendritic Cells Reveals Striking Species-Specific Differences.
    J Immunol. 197 (12): 4791-806.
  22. Suzuki, S. et al. (2016) Generation and characterization of RAG2 knockout pigs as animal model for severe combined immunodeficiency.
    Vet Immunol Immunopathol. 178: 37-49.
  23. LeLuduec, J.B. et al. (2016) Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.
    Vaccine. 34 (7): 914-22.
  24. Waddell, L.A. et al. (2018) ADGRE1 (EMR1, F4/80) Is a Rapidly-Evolving Gene Expressed in Mammalian Monocyte-Macrophages.
    Front Immunol. 9: 2246.
  25. Fernández-Caballero, T. et al. (2018) Phenotypic and functional characterization of porcine bone marrow monocyte subsets.
    Dev Comp Immunol. 81: 95-104.
  26. Loss, H. et al. (2018) Effects of a pathogenic ETEC strain and a probiotic Enterococcus faecium strain on the inflammasome response in porcine dendritic cells.
    Vet Immunol Immunopathol. 203: 78-87.
  27. Skovdal, S.M. et al. (2019) Inhaled nebulized glatiramer acetate against Gram-negative bacteria is not associated with adverse pulmonary reactions in healthy, young adult female pigs.
    PLoS One. 14 (10): e0223647.
  28. Ferret-Bernard, S. et al. (2020) Maternal Supplementation of Food Ingredient (Prebiotic) or Food Contaminant (Mycotoxin) Influences Mucosal Immune System in Piglets.
    Nutrients. 12 (7): 2115.
  29. Boettcher, A.N. et al. (2020) CD3ε(+) Cells in Pigs With Severe Combined Immunodeficiency Due to Defects in ARTEMIS.
    Front Immunol. 11: 510.
  30. Van der Weken, H. et al. (2021) Antibody-Mediated Targeting of Antigens to Intestinal Aminopeptidase N Elicits Gut IgA Responses in Pigs.
    Front Immunol. 12: 753371.
  31. Teuben, M. et al. (2021) Instant intra-operative neutropenia despite the emergence of banded (CD16dim/CD62Lbright) neutrophils in peripheral blood - An observational study during extensive trauma-surgery in pigs.
    Injury. 52 (3): 426-33.
  32. Teuben, M.P.J. et al. (2022) Standardized porcine unilateral femoral nailing is associated with changes in PMN activation status, rather than aberrant systemic PMN prevalence.
    Eur J Trauma Emerg Surg. 48 (3): 1601-11.
  33. Zhao, H. et al. (2022) Development of RAG2 -/- IL2Rγ -/Y immune deficient FAH-knockout miniature pig.
    Front Immunol. 13: 950194.
  34. Štěpánová, H. et al. (2022) Characterization of Porcine Monocyte-Derived Macrophages Cultured in Serum-Reduced Medium.
    Biology (Basel). 11(10):1457.
  35. Monguió-Tortajada, M. et al. (2022) Acellular cardiac scaffolds enriched with MSC-derived extracellular vesicles limit ventricular remodelling and exert local and systemic immunomodulation in a myocardial infarction porcine model.
    Theranostics. 12 (10): 4656-70.
  36. Haach, V. et al. (2023) A polyvalent virosomal influenza vaccine induces broad cellular and humoral immunity in pigs.
    Virol J. 20 (1): 181.
  37. Álvarez, B. et al. (2023) Porcine Macrophage Markers and Populations: An Update.
    Cells. 12 (16): 2103.
  38. Maciag, S. et al. (2022) Effects of freezing storage on the stability of maternal cellular and humoral immune components in porcine colostrum.
    Vet Immunol Immunopathol. 254: 110520.
  39. Forner, R. et al. (2021) Distribution difference of colostrum-derived B and T cells subsets in gilts and sows.
    PLoS One. 16 (5): e0249366.
  40. Muir, A. et al. (2024) Single-cell analysis reveals lasting immunological consequences of influenza infection and respiratory immunization in the pig lung.
    PLoS Pathog. 20 (7): e1011910.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.
  2. Gerner W et al. (2015) Phenotypic and functional differentiation of porcine αβ T cells: current knowledge and available tools.
    Mol Immunol. 66 (1): 3-13.

Flow Cytometry

Immunofluorescence

Immunomagnetic assay

Synonyms
FcRIII
RRID
AB_2104030
UniProt
Q28942
Entrez Gene
FCGR3B
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0019864 IgG binding

MCA1971F

150160 159305 168973

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