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CD16 antibody | G7

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MCA1971F
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F 0.1 mg loader
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Mouse anti Pig CD16, clone G7 recognizes porcine CD16 also known as Fc-gamma RIII or the low affinity IgG (Fc) receptor III. Clone G7 was clustered as CD16 at the Second International Workshop to Define Swine Cluster of Differentiation (CD) Antigens (Saalmuller et al. 1998).

Mouse anti pig CD16 immunoprecipitates a protein of ~40 kDa from porcine neutrophils and NK cells (Wierda et al. 1993). Subsequent cloning and characterization of the G7 molecule indicated that G7 was the porcine homologue of Human CD16 (Halloran et al. 1994).

Target Species
Pig
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Immunogen
Porcine peripheral blood leucocytes
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized Balb/c mice were fused with cells of the mouse P3-X63-Ag8.653 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to label 106 cells in 100μl

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
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Description Mouse IgG1 Negative Control:FITC

Source Reference

  1. Dato, M.E. et al. (1992) A triggering structure recognized by G7 monoclonal antibody on porcine lymphocytes and granulocytes.
    Cell Immunol. 140 (2): 468-77.

References for CD16 antibody

  1. Wierda, W.G. et al. (1993) Two distinct porcine natural killer lytic trigger molecules as PNK-E/G7 molecular complex.
    Cell Immunol. 146 (2): 270-83.
  2. Halloran, P.J. et al. (1994) Biochemical characterization of the porcine Fc gamma RIII alpha homologue G7.
    Cell Immunol. 158 (2): 400-13.
  3. Sánchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.
    J Immunol. 162 (9): 5230-7.
  4. Terzic, S. et al. (2002) Immunophenotyping of leukocyte subsets in peripheral blood and palatine tonsils of prefattening pigs.
    Vet Res Commun. 26: 273 - 83.
  5. Vincent, I.E. et al. (2003) Dendritic cells harbor infectious porcine circovirus type 2 in the absence of apparent cell modulation or replication of the virus.
    J Virol. 77: 13288 - 300.
  6. Summerfield, A. et al. (2003) Porcine peripheral blood dendritic cells and natural interferon-producing cells.
    Immunology 110: 440-9.
  7. Inman, C.F. et al. (2010) Rearing environment affects development of the immune system in neonates.
    Clin Exp Immunol. 160 (3): 431-9.
  8. Inman, C.F. et al. (2010) Dendritic cells interact with CD4 T cells in intestinal mucosa.
    J Leukoc Biol. 88 (3): 571-8.
  9. View The Latest Product References
  10. Devriendt, B. et al. (2010) Targeting of Escherichia coli F4 fimbriae to Fcgamma receptors enhances the maturation of porcine dendritic cells.
    Vet Immunol Immunopathol. 135 (3-4): 188-98.
  11. Gimeno, M. et al. (2011) Cytokine profiles and phenotype regulation of antigen presenting cells by genotype-I porcine reproductive and respiratory syndrome virus isolates.
    Vet Res. 42: 9.
  12. Mussá, T. et al. (2011) Interaction of porcine conventional dendritic cells with swine influenza virus.
    Virology 420: 125-34.
  13. Lecours, M.P. et al. (2011) Characterization of porcine dendritic cell response to Streptococcus suis.
    Vet Res. 42: 72.
  14. Mair, K.H. et al. (2012) NKp46 expression discriminates porcine NK cells with different functional properties.
    Eur J Immunol. 42: 1261-71.
  15. Hester, S.N. et al. (2012) Intestinal and systemic immune development and response to vaccination are unaffected by dietary (1,3/1,6)-β-D-glucan supplementation in neonatal piglets.
    Clin Vaccine Immunol. 19 (9): 1499-508.
  16. Thierry, A. et al. (2012) Identification of invariant natural killer T cells in porcine peripheral blood.
    Vet Immunol Immunopathol. 149 (3-4): 272-9.
  17. Inman, C.F. et al. (2012) Neonatal colonisation expands a specific intestinal antigen-presenting cell subset prior to CD4 T-cell expansion, without altering T-cell repertoire.
    PLoS One 7: e33707.
  18. Masure, D. et al. (2013) A Role for Eosinophils in the Intestinal Immunity against Infective Ascaris suum Larvae.
    PLoS Negl Trop Dis. 7: e2138.
  19. Mair, K.H. et al. (2013) Porcine CD8αdim/-NKp46high NK cells are in a highly activated state.
    Vet Res. 44: 13.
  20. Kyrova, K. et al. (2014) The response of porcine monocyte derived macrophages and dendritic cells to Salmonella typhimurium and lipopolysaccharide.
    BMC Vet Res. 10: 244.
  21. Waide, E.H. et al. (2015) Not All SCID Pigs Are Created Equally: Two Independent Mutations in the Artemis Gene Cause SCID in Pigs.
    J Immunol. 195 (7): 3171-9.
  22. Auray, G. et al. (2016) Characterization and Transcriptomic Analysis of Porcine Blood Conventional and Plasmacytoid Dendritic Cells Reveals Striking Species-Specific Differences.
    J Immunol. 197 (12): 4791-806.
  23. Suzuki, S. et al. (2016) Generation and characterization of RAG2 knockout pigs as animal model for severe combined immunodeficiency.
    Vet Immunol Immunopathol. 178: 37-49.
  24. LeLuduec, J.B. et al. (2016) Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.
    Vaccine. 34 (7): 914-22.
  25. Loss, H. et al. (2018) Effects of a pathogenic ETEC strain and a probiotic Enterococcus faecium strain on the inflammasome response in porcine dendritic cells.
    Vet Immunol Immunopathol. 203: 78-87.
  26. Fernández-Caballero, T. et al. (2018) Phenotypic and functional characterization of porcine bone marrow monocyte subsets.
    Dev Comp Immunol. 81: 95-104.
  27. Skovdal, S.M. et al. (2019) Inhaled nebulized glatiramer acetate against Gram-negative bacteria is not associated with adverse pulmonary reactions in healthy, young adult female pigs.
    PLoS One. 14 (10): e0223647.
  28. Ferret-Bernard, S. et al. (2020) Maternal Supplementation of Food Ingredient (Prebiotic) or Food Contaminant (Mycotoxin) Influences Mucosal Immune System in Piglets.
    Nutrients. 12 (7): 2115.
  29. Boettcher, A.N. et al. (2020) CD3ε(+) Cells in Pigs With Severe Combined Immunodeficiency Due to Defects in ARTEMIS.
    Front Immunol. 11: 510.
  30. Teuben, M. et al. (2021) Instant intra-operative neutropenia despite the emergence of banded (CD16dim/CD62Lbright) neutrophils in peripheral blood - An observational study during extensive trauma-surgery in pigs.
    Injury. 52 (3): 426-33.
  31. Van der Weken, H. et al. (2021) Antibody-Mediated Targeting of Antigens to Intestinal Aminopeptidase N Elicits Gut IgA Responses in Pigs.
    Front Immunol. 12: 753371.
  32. Zhao, H. et al. (2022) Development of RAG2 -/- IL2Rγ -/Y immune deficient FAH-knockout miniature pig.
    Front Immunol. 13: 950194.
  33. Štěpánová, H. et al. (2022) Characterization of Porcine Monocyte-Derived Macrophages Cultured in Serum-Reduced Medium.
    Biology (Basel). 11(10):1457.
  34. Monguió-Tortajada, M. et al. (2022) Acellular cardiac scaffolds enriched with MSC-derived extracellular vesicles limit ventricular remodelling and exert local and systemic immunomodulation in a myocardial infarction porcine model.
    Theranostics. 12 (10): 4656-70.
  35. Teuben, M.P.J. et al. (2022) Standardized porcine unilateral femoral nailing is associated with changes in PMN activation status, rather than aberrant systemic PMN prevalence.
    Eur J Trauma Emerg Surg. 48 (3): 1601-11.
  36. Haach, V. et al. (2023) A polyvalent virosomal influenza vaccine induces broad cellular and humoral immunity in pigs.
    Virol J. 20 (1): 181.
  37. Álvarez, B. et al. (2023) Porcine Macrophage Markers and Populations: An Update.
    Cells. 12 (16): 2103.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54.
  2. Gerner W et al. (2015) Phenotypic and functional differentiation of porcine αβ T cells: current knowledge and available tools.
    Mol Immunol. 66 (1): 3-13.

Flow Cytometry

Immunofluorescence

Immunomagnetic assay

Synonyms
FcRIII
RRID
AB_2104030
UniProt
Q28942
Entrez Gene
FCGR3B
GO Terms
GO:0005886 plasma membrane
GO:0016021 integral to membrane
GO:0004872 receptor activity
GO:0019864 IgG binding

MCA1971F

150160 159305

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