CD11R3 antibody | 2F4/11

Mouse anti Pig CD11R3:FITC

Product Type
Monoclonal Antibody

Product Code Applications Pack Size List Price Your Price Qty
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
F 0.1 mg loader
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Mouse anti Pig CD11R3, clone 2F4/11 recognizes porcine CD11R3, a ~155 kDa cell surface glycoprotein, member of the alpha integrin family.

Mouse anti Pig CD11R3, clone 2F4/11 was clustered as CD11R3 at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haverson et al. 2001). CD11R3 has a similar expression pattern to the human CD11b marker, being expressed on granulocytes, monocytes and alveolar macrophages, but not on lymphocytes, eythrocytes or platelets (Dominguez et al. 2001).

Mouse anti Pig CD11R3, clone 2F4/11 is reported to block phagocytosis of complement-opsonized zymosan particles by polymorphonuclear granulocytes and alveolar macrophages (Bullido et al. 1996).

Target Species
Product Form
Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% sodium azide (NaN3)
1% bovine serum albumin
Porcine alveolar macrophages
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Spleen cells from immunized BALB/c mice were fused with cells of the X63-Ag.8.653 myeloma cell line
Max Ex/Em
Fluorophore Excitation Max (nm) Emission Max (nm)
FITC 490 525
For research purposes only
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended. This product is photosensitive and should be protected from light.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
Flow Cytometry Neat 1/10
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.
Flow Cytometry
Use 10μl of the suggested working dilution to 1x106 cells in 100μl

Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse IgG1 Negative Control:FITC MCA928F F 100 Tests loader
List Price Your Price
Description Mouse IgG1 Negative Control:FITC

Source Reference

  1. Bullido R et al. (1996) Monoclonal antibody 2F4/11 recognizes the alpha chain of a porcine beta 2 integrin involved in adhesion and complement mediated phagocytosis.
    J Immunol Methods. 195 (1-2): 125-34.

References for CD11R3 antibody

  1. Sbrana, S. et al. (2014) Phenotype Changes of Circulating Monocytes in a Hypercholesterolemic Swine Model of Coronary Artery Disease
    J Cytol Histol 5:270
  2. Domínguez, J. et al. (2001) Workshop studies on monoclonal antibodies in the myeloid panel with CD11 specificity.
    Vet Immunol Immunopathol. 80 (1-2): 111-9.
  3. Sánchez-Torres C et al. (2003) Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection.
    Arch Virol. 148 (12): 2307-23.
  4. Van de Walle, G.R. et al. (2003) Transmission of pseudorabies virus from immune-masked blood monocytes to endothelial cells.
    J Gen Virol. 84 (Pt 3): 629-37.
  5. Alvarez, B. et al. (2000) Molecular and functional characterization of porcine LFA-1 using monoclonal antibodies to CD11a and CD18.
    Xenotransplantation 7: 258-266
  6. Sánchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.
    J Immunol. 162: 5230-7
  7. Thorgersen, E.B. et al. (2010) Anti-inflammatory effects of C1-Inhibitor in porcine and human whole blood are independent of its protease inhibition activity.
    Innate Immun. 16: 254-64
  8. Thorgersen, E.B. et al. (2010) CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs.
    FASEB J. 24: 712-22
  9. View The Latest Product References
  10. Baert K et al. (2015) Cell type-specific differences in β-glucan recognition and signalling in porcine innate immune cells.
    Dev Comp Immunol. 48 (1): 192-203.
  11. Barratt-Due, A. et al. (2011) Ornithodoros moubata Complement Inhibitor Is an Equally Effective C5 Inhibitor in Pigs and Humans.
    J Immunol. 187: 4913-9
  12. Jacobsen, M.J. et al. (2016) Altered Methylation Profile of Lymphocytes Is Concordant with Perturbation of Lipids Metabolism and Inflammatory Response in Obesity.
    J Diabetes Res. 2016: 8539057.
  13. Crisci, E. et al. (2012) Chimeric calicivirus-like particles elicit specific immune responses in pigs.
    Vaccine. 30 (14): 2427-39.
  14. Debeer, S. et al. (2013) Comparative histology and immunohistochemistry of porcine versus human skin.
    Eur J Dermatol. 23 (4): 456-66.
  15. Westover, A.J. et al. (2016) An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome.
    J Diabetes Res. 2016: 3486727.
  16. LeLuduec, J.B. et al. (2016) Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.
    Vaccine. 34 (7): 914-22.
  17. Grodeland, G. et al. (2020) Targeting of HA to chemokine receptors induces strong and cross-reactive T cell responses after DNA vaccination in pigs.
    Vaccine. 38 (6): 1280-1285.
  18. Teuben, M.P.J. et al. (2021) Standardized porcine unilateral femoral nailing is associated with changes in PMN activation status, rather than aberrant systemic PMN prevalence.
    Eur J Trauma Emerg Surg. Jun 10 [Epub ahead of print].
  19. Teuben, M. et al. (2021) Instant intra-operative neutropenia despite the emergence of banded (CD16dim/CD62Lbright) neutrophils in peripheral blood - An observational study during extensive trauma-surgery in pigs.
    Injury. 52 (3): 426-33.
  20. Zhou, L. et al. (2022) Clinical improvement of sepsis by extracorporeal centrifugal leukocyte apheresis in a porcine model.
    J Transl Med. 20 (1): 538.

Further Reading

  1. Piriou-Guzylack, L. (2008) Membrane markers of the immune cells in swine: an update.
    Vet Res. 39: 54

Flow Cytometry



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