CD11R3 antibody | 2F4/11

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Mouse anti Pig CD11R3:Alexa Fluor® 647

Product Type: Monoclonal Antibody
Clone: 2F4/11
Isotype: IgG1
Specificity: CD11R3

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References (21)
Applications
Storage Information
Negative Controls
Batch/Lot Specific Datasheets

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
MCA2309A647 Datasheet SDS SDS	F	100 Tests/1ml		Log in
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Anti Pig CD11R3 Antibody, clone 2F4/11 thumbnail image 1

Anti Pig CD11R3 Antibody, clone 2F4/11 thumbnail image 2

Anti Pig CD11R3 Antibody, clone 2F4/11 thumbnail image 3

Anti Pig CD11R3 Antibody, clone 2F4/11 thumbnail image 4

Anti Pig CD11R3 Antibody, clone 2F4/11 gallery image 1 — Figure A. APC conjugated Mouse anti Porcine CD31 antibody, clone LCI-4 (**MCA1746APC**) and FITC conjugated Mouse IgG1 isotype control (**MCA928F**).
Figure B. APC conjugated Mouse anti Porcine CD31 antibody, clone LCI-4 (**MCA1746APC**) and FITC conjugated Mouse anti Porcine CD11R3 antibody, clone 2F4/11(**MCA2309F**). All experiments performed on red cell lysed porcine blood gated on lymphocytes.
Data acquired on the **ZE5 Cell Analyzer**.

Anti Pig CD11R3 Antibody, clone 2F4/11 gallery image 2 — Figure A. FITC conjugated Mouse anti Porcine CD11R3 antibody, clone 2F4/11 (**MCA2309F**) and APC conjugated Mouse IgG1 isotype control (**MCA928APC**).
Figure B. FITC conjugated Mouse anti Porcine CD11R3 antibody, clone 2F4/11 (**MCA2309F**) and APC conjugated Mouse anti Porcine CD31 antibody, clone LCI-4 (**MCA1746APC**). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Data acquired on the **ZE5 Cell Analyzer**.

Anti Pig CD11R3 Antibody, clone 2F4/11 gallery image 3 — Figure A. APC conjugated Mouse anti Porcine CD31 (**MCA1746APC**) and FITC conjugated Mouse IgG1 isotype control (**MCA928F**).
Figure B. APC conjugated Mouse anti Porcine CD31 (**MCA1746APC**) and FITC conjugated Mouse anti Porcine CD11R3 (**MCA2309F**). All experiments performed on red cell lysed porcine blood gated on mononuclear cells.
Data acquired on the **ZE5 Cell Analyzer**.

Anti Pig CD11R3 Antibody, clone 2F4/11 gallery image 4 — **Published customer image:**
Mouse anti Porcine CD11R3 antibody, clone 2F4/11 (**MCA2309GA**) employed in the measurement of CD11R3 expression as a measure of neutrophil activation in a porcine model of metabolic syndrome by flow cytometry.
**Image caption:**
Systemic neutrophil activation can be evaluated by acute changes in CD11R3 expression and the percent circulating neutrophil population that is resistant to apoptosis *in vitro*. CD11R3 expression is increased acutely with neutrophil activation. With SCD exposure, CD11R3 expression decreased (a, n-6*, p=0.033). During the activated state neutrophil apoptosis is delayed. In these MetS animals, prior to SCD treatment, 34% of neutrophils are activated (resistant to apoptosis), while in healthy animals, less than 10% of circulating neutrophils are expected to be resistant to apoptosis. Analysis of apoptosis, before and after treatments, showed SCD Rx to move the neutrophil population to a less activated state, thus shifting toward naïve percentages of neutrophils resistant to apoptosis (b, n=9, p=0.023). For CD11R3 expression, only 2 of the 3 SCD Rx courses with three 6-hour treatments each were included in the analysis (n=6) due to an unanticipated analytic procedural complication.

From: Angela J. Westover, Kimberly A. Johnston, Deborah A. Buffington, and H. David Humes,
An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome.
**Journal of Diabetes Research, vol. 2016, Article ID 3486727, 10 pages, 2016**.
doi: 10.1155/2016/3486727.
This image is from an open access article distributed under the terms of a Creative Commons Attribution License.

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Mouse anti Pig CD11R3, clone 2F4/11 recognizes porcine CD11R3, a ~155 kDa cell surface glycoprotein, member of the alpha integrin family.

Mouse anti Pig CD11R3, clone 2F4/11 was clustered as CD11R3 at the Third International Workshop on Swine Leukocyte Differentiation Antigens (Haverson et al. 2001). CD11R3 has a similar expression pattern to the human CD11b marker, being expressed on granulocytes, monocytes and alveolar macrophages, but not on lymphocytes, eythrocytes or platelets (Dominguez et al. 2001).

Mouse anti Pig CD11R3, clone 2F4/11 is reported to block phagocytosis of complement-opsonized zymosan particles by polymorphonuclear granulocytes and alveolar macrophages (Bullido et al. 1996).

Target Species: Pig
Product Form: Purified IgG conjugated to Alexa Fluor® 647 - liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% sodium azide (NaN₃)
1% bovine serum albumin
Immunogen: Porcine alveolar macrophages
Approx. Protein Concentrations: IgG concentration 0.05 mg/ml
Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the X63-Ag.8.653 myeloma cell line
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

Alexa Fluor®647 650 665
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product is provided under an intellectual property licence from Life Technologies Corporation. The transfer of this product is contingent on the buyer using the purchased product solely in research, excluding contract research or any fee for service research, and the buyer must not sell or otherwise transfer this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; (c) manufacturing or quality assurance or quality control, or (d) resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad CA 92008 USA or outlicensing@thermofisher.com

Fluorophore	Excitation Max (nm)	Emission Max (nm)
Alexa Fluor®647	650	665

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry		Neat	1/10

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 10μl of the suggested working dilution to 1x10⁶ cells in 100μl

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Description	Product Code	Applications	Pack Size	Your Price
Mouse IgG1 Negative Control:Alexa Fluor® 647	MCA928A647	F	100 Tests/1ml	Log in
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Description	Mouse IgG1 Negative Control:Alexa Fluor® 647

Source Reference

Bullido R et al. (1996) Monoclonal antibody 2F4/11 recognizes the alpha chain of a porcine beta 2 integrin involved in adhesion and complement mediated phagocytosis.
J Immunol Methods. 195 (1-2): 125-34.

References for CD11R3 antibody

Sbrana, S. et al. (2014) Phenotype Changes of Circulating Monocytes in a Hypercholesterolemic Swine Model of Coronary Artery Disease
J Cytol Histol 5:270
Domínguez, J. et al. (2001) Workshop studies on monoclonal antibodies in the myeloid panel with CD11 specificity.
Vet Immunol Immunopathol. 80 (1-2): 111-9.
Sánchez-Torres C et al. (2003) Expression of porcine CD163 on monocytes/macrophages correlates with permissiveness to African swine fever infection.
Arch Virol. 148 (12): 2307-23.
Van de Walle, G.R. et al. (2003) Transmission of pseudorabies virus from immune-masked blood monocytes to endothelial cells.
J Gen Virol. 84 (Pt 3): 629-37.
Alvarez, B. et al. (2000) Molecular and functional characterization of porcine LFA-1 using monoclonal antibodies to CD11a and CD18.
Xenotransplantation 7: 258-266
Sánchez, C. et al. (1999) The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation.
J Immunol. 162: 5230-7
Thorgersen, E.B. et al. (2010) Anti-inflammatory effects of C1-Inhibitor in porcine and human whole blood are independent of its protease inhibition activity.
Innate Immun. 16: 254-64
Thorgersen, E.B. et al. (2010) CD14 inhibition efficiently attenuates early inflammatory and hemostatic responses in Escherichia coli sepsis in pigs.
FASEB J. 24: 712-22

View The Latest Product References

Baert K et al. (2015) Cell type-specific differences in β-glucan recognition and signalling in porcine innate immune cells.
Dev Comp Immunol. 48 (1): 192-203.
Barratt-Due, A. et al. (2011) Ornithodoros moubata Complement Inhibitor Is an Equally Effective C5 Inhibitor in Pigs and Humans.
J Immunol. 187: 4913-9
Jacobsen, M.J. et al. (2016) Altered Methylation Profile of Lymphocytes Is Concordant with Perturbation of Lipids Metabolism and Inflammatory Response in Obesity.
J Diabetes Res. 2016: 8539057.
Crisci, E. et al. (2012) Chimeric calicivirus-like particles elicit specific immune responses in pigs.
Vaccine. 30 (14): 2427-39.
Debeer, S. et al. (2013) Comparative histology and immunohistochemistry of porcine versus human skin.
Eur J Dermatol. 23 (4): 456-66.
Westover, A.J. et al. (2016) An Immunomodulatory Device Improves Insulin Resistance in Obese Porcine Model of Metabolic Syndrome.
J Diabetes Res. 2016: 3486727.
LeLuduec, J.B. et al. (2016) Intradermal vaccination with un-adjuvanted sub-unit vaccines triggers skin innate immunity and confers protective respiratory immunity in domestic swine.
Vaccine. 34 (7): 914-22.
Grodeland, G. et al. (2020) Targeting of HA to chemokine receptors induces strong and cross-reactive T cell responses after DNA vaccination in pigs.
Vaccine. 38 (6): 1280-1285.
Teuben, M.P.J. et al. (2021) Standardized porcine unilateral femoral nailing is associated with changes in PMN activation status, rather than aberrant systemic PMN prevalence.
Eur J Trauma Emerg Surg. Jun 10 [Epub ahead of print].
Teuben, M. et al. (2021) Instant intra-operative neutropenia despite the emergence of banded (CD16^dim/CD62L^bright) neutrophils in peripheral blood - An observational study during extensive trauma-surgery in pigs.
Injury. 52 (3): 426-33.
Zhou, L. et al. (2022) Clinical improvement of sepsis by extracorporeal centrifugal leukocyte apheresis in a porcine model.
J Transl Med. 20 (1): 538.