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Vwf antibody | E01-1C8

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Anti Human Vwf Antibody, clone E01-1C8 thumbnail image 1
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Sandwich ELISA analysis of human vWF using Mouse anti Human vWF (MCA6288GA) as a capture reagent and biotinylated Mouse anti Human vWF (MCA6289B) as a detection reagent with purified recombinant vWF as the antigen. Detection was performed using HRP conjugated streptavidin and substrate.

Mouse anti Human Vwf:Biotin

Product Type
Monoclonal Antibody
Clone
E01-1C8
Isotype
IgG2b
Specificity
Vwf

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Product Code Applications Pack Size List Price Your Price Qty
MCA6289B
Datasheet Datasheet Datasheet
SDS Safety Datasheet SDS
E 0.1 mg loader
List Price Your Price
loader

Mouse anti Human vWF antibody, clone E01-1C8, recognizes von Willebrand factor (vWF) a multifunctional glycoprotein that plays essential roles in primary and secondary homeostasis, mediates platelet adhesion and is a carrier of coagulation factor VIII (Yasar et al. 2018).

vWF is present in blood plasma, the subendothelial matrix, platelet granules (α granules) and endothelial cells (Kawecki et al. 2017). In endothelial cells, the vWF is stored in mature Weibel-Palade bodies before it is secreted into the plasma (Yasar et al. 2018). vWF plasma levels are influenced by genetic, pathological, hormonal and environmental interactions (Swystun & Lillicrap 2018). Upon vascular injury, vWF mediates the anchoring of platelets to the subendothelium leading to the formation of a platelet plug and the arrest of bleeding. Due to its acute release by the activated epithelium, vWF has been considered a marker of inflammation in various pathologies. However there is evidence that vWF is able to play a direct role in inflammation by recruiting leukokcytes to sites of inflammation (Kawecki et al. 2017).

The biotinylated Mouse anti Human vWF antibody, clone E01-1C8 (MCA6289B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human vWF antibody, clone C01-1F2 (MCA6288GA) as the capture antibody.

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
CHO derived recombinant full length human vWF (Met1-Lys2813)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
Regulatory
For research purposes only
Guarantee
12 months from date of despatch

This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

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Description Product Code Applications Pack Size List Price Your Price Quantity
Mouse anti Human Vwf MCA6288GA E 0.1 mg loader
List Price Your Price
loader
Description Mouse anti Human Vwf

UniProt
P04275
Entrez Gene
VWF
GO Terms
GO:0001948 glycoprotein binding
GO:0002020 protease binding
GO:0002576 platelet degranulation
GO:0051087 chaperone binding
GO:0005783 endoplasmic reticulum
GO:0005178 integrin binding
GO:0005518 collagen binding
GO:0005578 proteinaceous extracellular matrix
GO:0007597 blood coagulation, intrinsic pathway
GO:0019865 immunoglobulin binding
GO:0030168 platelet activation
GO:0031093 platelet alpha granule lumen
GO:0031589 cell-substrate adhesion
GO:0033093 Weibel-Palade body
GO:0042803 protein homodimerization activity
GO:0051260 protein homooligomerization
GO:0047485 protein N-terminus binding

MCA6289B

100005274

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