TIM-4 antibody | rAB04-3G2

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Mouse anti Human TIM-4:StarBright Violet 670

Product Type: Monoclonal Antibody
Clone: rAB04-3G2
Isotype: IgG1
Specificity: TIM-4

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Product Code Applications Pack Size List Price Your Price Qty

List Price	Your Price
TZA096SBV670 Datasheet SDS SDS	F *	100 Tests/0.5ml		Log in
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Anti Human TIM-4 Antibody, clone rAB04-3G2 thumbnail image 1

Anti Human TIM-4 Antibody, clone rAB04-3G2 thumbnail image 2

Anti Human TIM-4 Antibody, clone rAB04-3G2 thumbnail image 3

Anti Human TIM-4 Antibody, clone rAB04-3G2 thumbnail image 4

Anti Human TIM-4 Antibody, clone rAB04-3G2 thumbnail image 5

Anti Human TIM-4 Antibody, clone rAB04-3G2 thumbnail image 6

Anti Human TIM-4 Antibody, clone rAB04-3G2 thumbnail image 7

Anti Human TIM-4 Antibody, clone rAB04-3G2 gallery image 1 — HEK293 cells expressing human TIM-4 were stained with Anti-Human TIM-4 SBUV795 (**TZA096SBUV795**) (green line) or unstained (black line). Experiments were performed on TIM-4 transfected HEK293 cells gated on live, single cells. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human TIM-4 Antibody, clone rAB04-3G2 gallery image 2 — HEK293 cells expressing human TIM-4 were stained with Anti-Human TIM-4 SBV515 (**TZA096SBV515**) (green line) or unstained (black line). Experiments were performed on TIM-4 transfected HEK293 cells gated on live, single cells. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human TIM-4 Antibody, clone rAB04-3G2 gallery image 3 — HEK293 cells expressing human TIM-4 were stained with Anti-Human TIM-4 SBV610 (**TZA096SBV610**) (green line) or unstained (black line). Experiments were performed on TIM-4 transfected HEK293 cells gated on live, single cells. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human TIM-4 Antibody, clone rAB04-3G2 gallery image 4 — HEK293 cells expressing human TIM-4 were stained with Anti-Human TIM-4 SBB580 (**TZA096SBB580**) (green line) or unstained (black line). Experiments were performed on TIM-4 transfected HEK293 cells gated on live, single cells. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human TIM-4 Antibody, clone rAB04-3G2 gallery image 5 — HEK293 cells expressing human TIM-4 were stained with Anti-Human TIM-4 SBB615 (**TZA096SBB615**) (green line) or unstained (black line). Experiments were performed on TIM-4 transfected HEK293 cells gated on live, single cells. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human TIM-4 Antibody, clone rAB04-3G2 gallery image 6 — HEK293 cells expressing human TIM-4 were stained with Anti-Human TIM-4 SBY720 (**TZA096SBY720**) (green line) or unstained (black line). Experiments were performed on TIM-4 transfected HEK293 cells gated on live, single cells. Data were acquired on the **ZE5 Cell Analyzer**.

Anti Human TIM-4 Antibody, clone rAB04-3G2 gallery image 7 — HEK293 cells expressing human TIM-4 were stained with Anti-Human TIM-4 SBUV445 (**TZA096SBUV445**) (green line) or unstained (black line). Experiments were performed on TIM-4 transfected HEK293 cells gated on live, single cells. Data were acquired on the **ZE5 Cell Analyzer**.

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Mouse anti Human TIM-4 antibody, clone rAB04-3G2, is a recombinant monoclonal antibody that recognizes TIM-4, also known as SMUCKLER, T-cell immunoglobulin and mucin domain-containing protein 4 or TIMD-4.

TIM-4 is a ~ 42 kDa type I membrane protein which is preferentially expressed on macrophages, mature dendritic cells, peritoneal B1 cells and invariant natural killer T (iNKT) cells. TIM-4 is the ligand of TIM-1, and binding leads to T-cell proliferation and cytokine production.

Polymorphisms in the TIM-4 gene may be associated with a predisposition to asthma. TIM-4 is expressed in various tumor cells, including lung cancer, colorectal cancer, and certain sarcomas. It can contribute to tumor growth and immune evasion by modulating the tumor microenvironment and promoting angiogenesis.

Target Species: Human
Product Form: Purified recombinant IgG containing a SpyTag at the C-terminus of the antibody heavy chain and conjugated to StarBright Violet 670 – liquid
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Source: CHO cells
Buffer Solution: Phosphate buffered saline
Preservative Stabilisers: 0.09% Sodium Azide (NaN₃)
1% Bovine Serum Albumin
0.1% Pluronic F68
0.1% PEG 3350
0.05% Tween 20
Immunogen: Recombinant TIM-4 Glu25-Gln314 expressed by mammalian cells
Approx. Protein Concentrations: For information on the concentration of our StarBright Dye conjugated reagents please visit our FAQ page.
Max Ex/Em: Fluorophore Excitation Max (nm) Emission Max (nm)

StarBright Violet 670 401 667
Regulatory: For research purposes only
Guarantee: 12 months from date of despatch
Acknowledgements: This product and/or its use is covered by claims of U.S. patents, and/or pending U.S. and non-U.S. patent applications owned by or under license to Bio-Rad Laboratories, Inc. See bio-rad.com/en-us/trademarks for details.
This product is covered by U.S Patent No. 10,150,841 and related U.S and foreign counterparts.

Store at +4°C. DO NOT FREEZE. This product should be stored undiluted.

This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Verified	Min Dilution	Max Dilution
Flow Cytometry ¹		Neat

¹Staining at lower temperatures generally requires longer incubation to reach antibody binding equilibrium, and this effect is more pronounced for StarBright Dyes due to their larger size. For optimum staining, Bio-Rad recommends incubating samples either overnight at 4°C or 1 hour at room temperature.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Flow Cytometry: Use 5μl of the suggested working dilution to label 0.5x10⁶ cells in 100μl. Best practices suggest a 5 min centrifugation at 6,000g prior to sample application.

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Synonyms: Smuckler
UniProt: Q96H15
Entrez Gene: TIMD4
GO Terms: GO:0016021 integral to membrane