Osteopontin antibody | F03-4E8

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Mouse anti Human Osteopontin:Biotin

Product Type
Monoclonal Antibody
Clone
F03-4E8
Isotype
IgG1
Product Code Applications Pack Size List Price Quantity
MCA6263B
Datasheet
E
SDS
0.1 mg loader

Mouse anti Human osteopontin antibody, clone F03-4E8, recognizes osteopontin (OPN) also known as bone sialoprotein 1, nephropontin secreted phosphoprotein 1 and uropontin. It is an acidic glycoprotein and is a member of the intrinsically disordered class of proteins (Kurzbach et al. 2013). OPN plays a role in a multitude of physiological and pathophysiological processes. In inflammation it induces cell adhesion and migration, regulates differentiation of proinflammatory lymphocytes and inhibits apoptosis of inflammatory cells (Clemente et al. 2016). OPN is expressed by various cell types including bone, neural, epithelial and endothelial cells. It is also expressed by the immune cells T cells, B cells, natural killer cells and macrophages. In its secreted form OPN can be found in biological fluids such as blood, urine, bile and milk (Castello et al. 2017). OPN has been found to play a role in cancer progression by enhancing proliferation, survival, motility and invasion of tumor cells in a range of cancers. OPN was upregulated at tumor sites and in blood of the patients, with the level correlating with tumor stage and aggressiveness (Castello et al. 2017). High levels of OPN have also been detected in several autoimmune diseases including multiple sclerosis and rheumatoid arthritis (Clemente et al. 2016).

The biotinylated Mouse anti Human osteopontin antibody, clone F03-4E8 (MCA6263B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human osteopontin antibody, clone F11-4C11 (MCA6262GA) as the capture antibody.

Product Details

Target Species
Human
Product Form
Purified IgG conjugated to Biotin - liquid
Preparation
Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
Buffer Solution
Phosphate buffered saline
Preservative Stabilisers
0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
Immunogen
HEK293 derived recombinant human full length osteopontin (Ile17-Asn314)
Approx. Protein Concentrations
IgG concentration 0.1 mg/ml
Fusion Partners
Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0

Storage Information

Storage
This product is shipped at ambient temperature. It is recommended to aliquot and store at -20°C on receipt. When thawed, aliquot the sample as needed. Keep aliquots at 2-8°C for short term use (up to 4 weeks) and store the remaining aliquots at -20°C.

Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
Guarantee
12 months from date of despatch

More Information

UniProt
P10451
Entrez Gene
SPP1
GO Terms
GO:0007155 cell adhesion
GO:0005125 cytokine activity
GO:0005615 extracellular space
GO:0007566 embryo implantation
GO:0031214 biomineral tissue development
GO:0033280 response to vitamin D
GO:0046697 decidualization
Regulatory
For research purposes only

Applications of Osteopontin antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
Application Name Verified Min Dilution Max Dilution
ELISA 1.0 ug/ml
Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Useful Reagents Available

Description Product Code Applications Pack Size List Price Quantity
Mouse anti Human Osteopontin MCA6262GA E 0.1 mg loader

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