Osteopontin antibody | F03-4E8
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|Mouse anti Human osteopontin antibody, clone F03-4E8, recognizes osteopontin (OPN) also known as bone sialoprotein 1, nephropontin secreted phosphoprotein 1 and uropontin. It is an acidic glycoprotein and is a member of the intrinsically disordered class of proteins (Kurzbach et al. 2013). OPN plays a role in a multitude of physiological and pathophysiological processes. In inflammation it induces cell adhesion and migration, regulates differentiation of proinflammatory lymphocytes and inhibits apoptosis of inflammatory cells (Clemente et al. 2016).
OPN is expressed by various cell types including bone, neural, epithelial and endothelial cells. It is also expressed by the immune cells T cells, B cells, natural killer cells and macrophages. In its secreted form OPN can be found in biological fluids such as blood, urine, bile and milk (Castello et al. 2017).
OPN has been found to play a role in cancer progression by enhancing proliferation, survival, motility and invasion of tumor cells in a range of cancers. OPN was upregulated at tumor sites and in blood of the patients, with the level correlating with tumor stage and aggressiveness (Castello et al. 2017). High levels of OPN have also been detected in several autoimmune diseases including multiple sclerosis and rheumatoid arthritis (Clemente et al. 2016).
The biotinylated Mouse anti Human osteopontin antibody, clone F03-4E8 (MCA6263B) can be used as a detection antibody in a sandwich ELISA with the purified Mouse anti Human osteopontin antibody, clone F11-4C11 (MCA6262GA) as the capture antibody.
- Target Species
- Product Form
- Purified IgG conjugated to Biotin - liquid
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- Buffer Solution
- Phosphate buffered saline
- Preservative Stabilisers
- 0.09% Sodium Azide (NaN3)
1% Bovine Serum Albumin
- HEK293 derived recombinant human full length osteopontin (Ile17-Asn314)
- Approx. Protein Concentrations
- IgG concentration 0.1 mg/ml
- Fusion Partners
- Cell fusion between immunized BALB/c mouse spleen cells and mouse myeloma SP2/0
- For research purposes only
- 12 months from date of despatch
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
|Application Name||Verified||Min Dilution||Max Dilution|
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