Ki67 antibody | AbD02531_h/m_IgG1

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Anti Ki67 Antibody, clone AbD02531_h/m_IgG1 gallery image 1 — Paraffin embedded Human Tonsil stained with Human anti Ki67 antibody, clone AbD02531 (HCA053)

Human anti Ki67

Product Type: Monoclonal Antibody
Clone: AbD02531_h/m_IgG1
Isotype: HuCAL/mouse IgG1 Fc

Product Code	Applications	Pack Size	Your Price	List Price	Quantity
HCA053 Datasheet	C E IF P* WB SDS	0.1 mg	Log in		Get Quote

Human anti Ki67 antibody, clone AbD02531 recognizes the Ki67 cell-cycle associated protein. Ki67 is expressed in proliferating cells but not in quiescent cells. Expression of this antigen occurs preferentially during late G1, S, G2, and M phases of the cell cycle, while in cells in G0 phase the antigen cannot be detected. Consequently, Ki-67 antigen expression is used in tumor pathology to detect proliferating cells in neoplastic diseases. In cultured cells, Ki-67 is expressed in the nucleolus of interphase cells. The Ki67 gene contains 15 exons. The Ki67 repeat region, within which there is a 22-amino acid Ki67 motif, is encoded by exon 13. The shorter isoform lacks exon 7. Northern blot analysis reveals multiple transcripts ranging from approximately 8.9 to 12.5 kb in proliferating but not quiescent cells. Immunoblot analysis shows expression of 320 and 359 kDa proteins. Antisense oligonucleotides inhibit cellular proliferation in a dose-dependent manner, suggesting that Ki67 protein expression may be an absolute requirement for cell proliferation.

Within cells Ki67 is predominantly localized in the G1 phase in the perinucleolar region, in the later phases it is also detected throughout the nuclear interior, being predominantly localized in the nuclear matrix. In mitosis, it is present on all chromosomes.

Product Details

Target Species

Human

Species Cross-Reactivity

Target Species	Cross Reactivity
Bovine	Expected from Sequence
Macaque	Expected from Sequence
Dog	Expected from Sequence
Chimpanzee	Expected from Sequence
Rhesus Monkey	Expected from Sequence

N.B. Antibody reactivity and working conditions may vary between species.

Product Form

Chimeric human-mouse IgG1 antibody selected from the HuCAL® phage display library and expressed in a human cell line. The antibody has variable regions of human origin and an Fc portion (including CH1 and CL domains) from mouse IgG1. It can be detected by anti mouse Fc specific secondary antibodies. This antibody is supplied liquid.

Preparation

Purified IgG prepared by affinity chromatography on Protein A

Buffer Solution

Phosphate buffered saline

Preservative Stabilisers

0.01% Thiomersal

Immunogen

Peptide derived from the human Ki67 protein, sequence GFKELFQTPG, coupled via a C-terminal cysteine to carrier proteins

Approx. Protein Concentrations

IgG concentration 0.5 mg/ml

Storage Information

Storage: Store at +4^oC or at -20^oC if preferred.
Storage in frost-free freezers is not recommended.
This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.
Guarantee: 12 months from date of despatch

More Information

UniProt: P46013
Entrez Gene: MKI67
GO Terms: GO:0005524 ATP binding; GO:0005730 nucleolus; GO:0008022 protein C-terminus binding; GO:0008283 cell proliferation
Acknowledgements: Sold under license of U.S. Patents 6753136, 7785859 and 8273688 and corresponding patents. This antibody was developed by Bio-Rad, Zeppelinstr. 4, 82178 Puchheim, Germany.
Licensed Use: For in vitro research purposes only, unless otherwise specified in writing by Bio-Rad.
Regulatory: For research purposes only

Applications of Ki67 antibody

This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.

Application Name	Min Dilution	Max Dilution
ELISA		2.0 ug/ml
Immunofluorescence
Immunohistology - Frozen
Immunohistology - Paraffin ¹	0.2 ug/ml	1.0 ug/ml
Western Blotting

¹This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose.

Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.

Technical Advice: Recommended protocols and further information about HuCAL recombinant antibody technology can be found in the HuCAL Antibodies Technical Manual
Histology Positive Control Tissue: Human tonsil

Secondary Antibodies Available

Description	Product Code	Applications	Pack Size	Your Price	List Price	Quantity
Goat anti Human IgG F(ab')2:HRP	0500-0099	E WB	0.5 ml	Log in		Get Quote
Histar Detection System	STAR3000A	C P	50 Tests	Log in		Get Quote

Product Specific References

References for Ki67 antibody

Jarutat, T. et al. (2006) Isolation and comparative characterization of Ki-67 equivalent antibodies from the HuCAL phage display library.
Biol. Chem. 387: 995-1003.
Liu, S.K. et al. (2011) Delta-like ligand 4-notch blockade and tumor radiation response.
J Natl Cancer Inst. 103 (23): 1778-98.

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